... Technology ix SPONSORS Chinese Academy of Engineering, Mechanical and Vehicle Engineering Division Chinese Society of Naval Architects and Marine Engineers China Institution of Navigation Ship Mechanics ... a professional discipline and science by exchanging knowledge and promoting discussion of relevant topics in the fields of naval architecture and marine and offshore engineering Inline with the ... University Prof Chang-Jian Weng, Wuhan University of Technology Prof Fei Xia, WuhanShip Design Institute Prof Zuo-Shui Xie, Hua Dong ShipbuildingInstitute Prof Zao-Jian Zou, Wuhan Universityof Technology...
... immunoprecipitated with an anti-FGFR3 serum then immunoblotted with anti-ubiquitin and anti-FGFR3 sera (D) Disabling the c-Cbl ubiquitylating activity does not affect the ubiquitylation of the wild-type, ... membrane The E3-ubiquitin ligase c-Cbl is directly involved in the ubiquitylation of several RTKs [24–26,32] and may participate in the downregulation of FGFR1 via an indirect interaction with the phosphorylated ... with chloroquine as indicated Lysates were immunoprecipitated and processed for immunoblotting with anti-ubiquitin and anti-FGFR3 sera (C) Ubiquitylation of the wild-type receptor is increased by...
... additional amino acid residues are also found in the DNases Iof amphibians [19] As in amphibian DNases I, the insertion of an additional amino acid residue into the shark enzymes may be essential ... (snake) and Osteichthyes (carp) enzymes were tested for crossinhibition of activity, all five antibodies were ineffective against the shark DNase I, indicating that, from an immunological standpoint, ... Hosomi, O., Nakashima, Y & Kishi, K (1997) Rabbit DNase I purification from urine, immunological and proteochemical characterization, nucleotide sequence, expression in tissues, relationships with...
... N-domain and the regulatory TnC-binding site [16] This interaction induces the dissociation of the inhibitory region, which is adjacent to the regulatory TnC-binding site, from actin, resulting in ... 33 Nishimura Y, Ojima T & Nishita K (1997) Bivalve tropomyosins exhibit strong inhibition of actomyosin Mg-ATPase and high viscosity Fish Sci 63, 802–806 34 Ojima T & Nishita K (1988) Separation ... the C-terminal region plays a role in the inhibition ⁄ removal of inhibition by alternative binding, while the N-terminal region is responsible for the Ca2+-dependent activation This view replaces...
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... cut with either PstI and EcoRI in case of sipV or EcoRI-SacI in case of sipW and ligated into corresponding sites of pUC18 DNA The ligation mixes were used for PCR with oligo nucleotides Uni1 or...
... surprising insofar as structurally defined Cu (I) -containing species were identified with such unexpectedly low stoichiometries of Cu (I) to polypeptide Several different Cu (I) binding stoichiometries ... and distinct from their Zn(II)-containing forms The spectra of both the only Zn(II)-containing b-domain and its Cu (I) 4 derivative seem to be of lower quality with large areas of broad overlapping ... Enhanced neurotrophic activity in Alzheimer’s disease cortex is not associated with downregulation of metallothionein-III (GIF) Brain Res 649, 297–304 Uchida Y, Takio K, Titani K, Ihara Y & Tomonaga...
... chips and with 0.3 M NaCl in the case of immobilized type VI collagen To form a complex consisting of decorin, type Iand type VI collagen, type VI collagen was first immobilized After binding of ... glycosaminoglycan chain; type I collagen, black circle; type VI collagen, white cylinder (A) Interaction involving dimerization of decorin and binding of both collagens to the inner surface of decorin ... was higher than that of the dimeric complex of decorin with type I or type VI collagen The existence of an interaction of decorin with type Iand type VI collagen has been shown in vivo in skin...
... (1993) Characterization of cathepsin B specificity by site-directed mutagenesis Importance of Glu245 in the S2–P2 specificity for arginine and its role in transition state stabilization J Biol Chem 268, ... and by affinity purification, followed by elution of high proteolytic activity [18] This indicates that N-terminal and C-terminal truncation of rPhl p is a prerequisite for its proteolytic activity ... molecular size is indicated Fig Three-dimensional and Prosite motifs of CBDs, proteinases and expansins (A) The consensus pattern of bacterial CBD (I, Prosite PS00561) and CBDs of fungi (II, Prosite...
... R Vile Middle Tennessee State University Diane E Wall Mississippi State University Mark David Welton United States Military Academy John Winkle University of Mississippi ix This page intentionally ... and culture Our goal in writing this textbook is to illustrate this premise in the context of the most salient and important provisions of the Constitution Volumes Iand II of American Constitutional ... is Alumni Distinguished Service Professor of Political Science and Resident Scholar of Constitutional Law in the College of Law at the University of Tennessee He holds a Ph.D in political science...
... metabolism The reduction in d-amino acid oxidase expression in the kidney proteome is interesting, in view of the contribution of this enzyme to glyoxylate production from glycine Thus, it could ... (2001) Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption ⁄ ionization-time of flight analysis Proteomics 1, 1359–1363 13 Sambrook ... fractions because of peroxisomal fragility With this limitation, our fractionation method was mainly useful as a simple way to reduce the complexity of the proteome, facilitating the differential...
... similarly, with no effect by inhibiting PLC-b (Fig 5A), strong down-regulation of collagen I levels after inhibition of PC-PLC (Fig 5B), and similar collagen I down-regulation after PLD inhibition ... regulating the induction of collagen I synthesis by CTGF Further examination of the intracellular signalling mechanisms revealed an essential involvement of Gai, PLD and PC-PLC Their inhibition ... regulates proliferation by proand antimitogenic actions [24,61] In addition, the ETA-receptor is a regulator of collagen I homeostasis by inducing both, its synthesis and its degradation [7,16] in contrast...
... order: iron(II)HbIV > met-HbIV > met-HbI > iron(II)-HbI The larger peroxidative activity of iron(II)-HbIV with respect to metHbIV is in line with previous data obtained with human HbA derivatives [7] ... nonperpendicular iron–ligand bond above the xy plane of the heme group Fig Circular dichroism spectra in the visible region of iron(II)-HbIV (A), iron(II)-HbI (B), met-HbIV (C) and met-HbI (D) in 50 ... [6] Figure shows the peroxidase activity of iron(II)- and met-forms of HbI and HbIV It is evident that the enzymatic activity decreases according to the order iron(II)-HbIV > met-HbIV > met-HbI...
... activity In this case, biological activity is expressed in terms of the ability of the modified TnI to modulate Ca2+ regulation of myofibrillar ATPase activity Following extraction of TnC and TnI from ... cTnC (wild type) and mutants (Site II inactive, Site I active, and Site I active but Site II inactive), Putkey et al [35] found that substitution with all of these cTnCs can only partially restore ... sites (I and II) bind Ca2+ exclusively but with lower affinity (K % 105 M)1) Binding of Ca2+ to the N-terminal sites is responsible for activation of actomyosin ATPase activity Due to some amino...
... characterize the composition of this minimal PSII identified in viridis zb63, thylakoids isolated from the mutant were analysed by SDS ⁄ PAGE and immunoblotting with antibodies specific for PSI and PSII ... PSII RC was already at a minimal level and did not change significantly even at high light intensity The inability of the viridis zb63 PSII mutant to adjust its antenna size to different light intensities ... that the minimal LHCII–PSII supercomplex binds about 100 Chls per PSII RC The binding of the additional LHCII trimers to this minimal PSII unit provides an outer peripheral system that increases...
... co-ordination of Chl might be in part different in PSI and in PSII; PSI binds Chls both within individual pigment binding proteins and at the interface between subunits In PSII, Chls are tightly ... pigments; (ii) monomeric Lhc; (iii) trimeric LHCII; (iv) Dimeric PSII core and (iv) PSII supercomplexes still intact Clearly, the fraction of chlorophylls liberated during the separation is far lower ... SDS/PAGE analysis (not shown) and identified as: (i) free pigments; (ii) dimeric LHCI; (iii) PSI-core and (iv) undissociated PSI–LHCI complex It is interesting to note that Lhca polypeptides were not...
... CFO and I/ Q mismatch estimation algorithms to be presented, only the periodicity of the baseband signal is required and exploited, and the detailed information of xI (n) and xQ (n) is not required ... which is free of I/ Q mismatch interference, estimating I/ Q mismatch using the estimated cosine value, and forming a sine estimator for CFO by removing I/ Q mismatch in the received signal using ... mismatch is only contained in xI (n) and xQ (n) If the channel is static during CFO estimation, periodically transmitted training symbols lead to periodical xI (n) and xQ (n) at the receiver In...
... considerably lower than what might have been expected considering the qualities (IRRs) of the individual radios alone This is indeed due to the interaction of the individual radio impairments and ... system-level tool in the system design and dimensioning Similar illustration is given in Figure 11 in which also the effects of using the pilot-based channel estimates in the diversity combining stage ... processing, the role of the RF impairments is likely to be more critical than in many traditional existing wireless systems This is indicated by the preliminary studies of the field [12–18] One important...
... Advances in Pediatrics 43 (1996): 127–170 indigestion See GASTROINTESTINAL DISORDERS individuality See BIOCHEMICAL INDIVIDUALITY induction The increased production of an in response to external stimuli ... function Vitamin B6 plays an important role in maintaining optimal immunity, including antibody production and phagocytic activity Vitamin B6 deficiency impairs the immune system in a number of ... the site of infection and inflammation Interstitial fluid, together with blood and lymph, help maintain the internal environment within normal limits When interstitial fluid flows through lymphatic...
... Brownian motions Part II: Combinatorial differential calculus In preparation, 2006 ´ Artur Je˙ and Piotr Sniady Generalized cauchy identities, trees and multiz dimensional Brownian motions Part III: ... central role in this series of articles 1.6.2 ´ Part II: Combinatorial differential calculus [JS06a] The bijections considered in Part Iof this series (Section and Section of this article) are far ... The main bijection The main result of this section is the algorithm MainBijection(T ) (with the auxiliary algorithm SmallBijection(T )) which provides the bijection announced in Theorem In the...
... amount of information in an X-ray density profile is simple, but seems to give far better results than trying to relate the classical within-ring parameters with the MOE (tables VI and VII): it is ... (table II) The remaining variability for E is low (as stated by Zobel and Van Buijtenen [51] and Cornelius [11], the variability for wood quality traits is often lower than the variability for ... of density values were calculated: 1) called here classical within-ring density parameters (ring width and ring density, minimum and maximum ring density, early and latewood width and early and...