... study of exotic and native herbaceous species In that case a process of information exchange and the compiled database was provided to a team of experts within the project team and international forage ... number of point records for specimen collection for each species within the study region and within each rainfall and soil band was totalled This provided an estimate of the frequency of occurrence ... material 3 In Vitro Methods for Direct Activity Against Ruminant Gastrointestinal Nematodes 33 In Vitro Screening A number of different bioassays using either the pre-parasitic stages of nematodes...
... (20 02) and Tunnicliffe et al (20 03), and by Kojima (20 02) for western Pacific seeps Seep microbiology is reviewed in Valentine & Reeburgh (20 00), Hinrichs & Boetius (20 02) and Valentine (20 02) ... quantitative sampling of associated fauna In uence of seep clams on associated infauna is discussed later in the section on macrofauna Seep infauna Foraminifera Interest in Foraminifera at methane ... (Phalascosoma) Densities of many taxa increase with habitat complexity, measured as tubeworm density, but decline with age of the tubeworm aggregation Increasing patch age leads to a decline in...
... Polymers and other chemicals 24 3 .2 Methods 24 3 .2. 1 Preparation of paper and composite samples 24 3 .2. 2 Measurement of paper strength development during drying 25 3 .2. 3 ... excessive shrinkage and unevenness of the films 3 .2. 2 Measurement of paper strength development during drying The development of paper strength during drying (Papers I and III) was measured with an ... adsorption methodof CMC has been pointed out in regard to fibre swelling and paper strength (Laine et al 20 02) In addition, the increase of specific surface of wet fibres by CMC adsorption has been...
... SERR and SEIRA spectroscopy of cytochrome c 20 21 22 23 24 25 26 27 28 29 30 31 nate creates an IR probe for electric fields in proteins J Am Chem Soc 128 , 13356–13357 Suydam IT & Boxer SG (20 03) ... Lys86, and Lys87, is associated with a distinctly weaker average electronic coupling of the haem with the electrode than the medium-affinity binding domain involving Lys25 and Lys27 As long as the ... case in the low-field regime, as demonstrated by the comparison of the electron transfer and reorientation rate constants [28 ] With increasing field strength, protein reorientation is increasingly...
... motif In EMSA assay, a single protein–DR1 1579 Interaction of ultraspiracle and ecdysone receptor in hormone signaling A B C F Fang et al D Fig Binding of ultraspiracle (USP) to DR1 motif and to ... dependence of JH III action to increase the transcriptional induction of lm 20 OHE As shown in Fig 4A, the region of the dose-dependent JH III action to increase FEBS Journal 27 2 (20 05) 1577–1589 ª 20 05 ... Transfection of increasing concentrations of dUSP-expressing plasmid, while increasing the transcriptional activation arising from treatment with JH III alone (d), does not result in enhancement of 20 OHE-activation...
... understanding of the biological basis of the genetic associations of this region with autoimmune rheumatic diseases Competing interests The authors declare that they have no competing interests ... therefore be required as initial steps in determining its potential role in modulating PTPN11 expression An alternative explanation is that a polymorphism within an enhancer sequence in the intergenic ... et al and inflammatory bowel disease [2] Approximately 20 % of pseudogenes are transcribed, andsome generate siRNAs that target homologous genes [8] Sequence variability in the sequence of the...
... 2. 1.4 .2 Electron Mediation 2. 1.4.3 Other Miscellaneous Applications 2.2 Conducting Polymers 2. 2.1 Structure and Synthesis 2. 2.1.1 Polyaniline ii Table of Contents 2. 2.1 .2 Polypyrrole 2.2 .2 Doping of ... Conducting Polymers 2. 2.3 Degradation and Stability 2. 2.4 Applications of Conducting Polymers 2. 2.4.1 Antistatic Coatings 2. 2.4 .2 Electromagnetic Shielding 2. 2.4.3 Organic Conducting Patterns 2. 2.4.4 ... electrochemical methods, and potential applications 23 Chapter 2. 2.1.1 Polyaniline The base form of polyanilines has the generalized formula as shown in Figure 2. 7, where someof the C6H4 rings are in the...
... Cu2+ 0.5 mM H2O2 WT 1× WT with stress 107 Δ coq7 Δ coq7 with stress Δ coq3 Δ coq3 with stress × 106 Δ cyc1 Δ cyc1 with stress × 105 12 16 20 24 28 32 (h ) 12 16 20 24 28 32 Fig Sensitivity of ... occurred in all ubiquinone-deficient strains, induction of gpx1+ was not observed in any of the tested strains (Fig 6) However, in the wild-type strain treated with mm H2O2 for 15 min, a high level of ... cysteine, and their growth was compared (B) Ubiquinone (UQ) was extracted from the same strains wild-type strain, in LN9 02 harboring pREP1–coq7Sp, andin LN9 02 harboring pREP1–TPCOQ7, whereas demethoxyubiquinone-10...
... helices of CA (C1 in teal, C2 in orange, and C3 in blue) are also indicated structs localized sites of interaction in Gag and LysRS to residues 308–3 62 at the C-terminal end of CA and 20 8 25 9 of the ... LysRS binding Residues T210, M214 and M215, along with a nearby H 226 , were implicated as critical by peptide binding studies and alanine scanning mutagenesis [23 ] Computational docking and biochemical ... synthetase to FEBS Journal 27 6 (20 09) 6118–6 127 ª 20 09 The Authors Journal compilation ª 20 09 FEBS A P Mascarenhas and K Musier-Forsyth 13 14 15 16 17 18 19 20 21 22 23 24 facilitate tRNA(Lys) incorporation...
... (1997) Predominant expression of monoamine oxidase B isoform in rabbit renal proximal tubule: 1574 19 20 21 22 23 24 25 26 27 28 29 30 31 regulation by I -2 imidazoline ligands in intact cells ... cavity and the active site [15] On the other hand, inhibition of MAO with a mechanismbased irreversible inhibitor did not prevent binding of the I2 ligand 2- (2- benzofuranyl) -2- imidazoline (2- BFI), ... Parini A & Lanier SM (1995) Imidazoline ⁄ guanidinium binding domains on monoamine oxidases ) relationship to subtypes of imidazoline-binding proteins and tissue-specific interaction of imidazoline...
... be involved in HD The results for similar motifs identified in the caspase-1 (for reference) caspase-3, caspase-7, caspase-8 and caspase-10 genes are shown in the Table In Table 3, the start and ... decrease in the intrinsic fluorescence of GST–HIPPI protein, indicating binding of the protein with this mutated motif (Fig 1B, panel II) The apparent dissociation constant (Kd) of this binding was ... shown) Increase in caspase-1 gene expression and induction of apoptosis by C-terminal pDED of HIPPI The role played by pDED of HIPPI in alteration of caspase-1 gene expression in HeLa cells was monitored...
... cylinder (A) Interaction involving dimerization of decorin and binding of both collagens to the inner surface of decorin (B) Interaction of type I collagen with the inner surface of the decorin ... Concentrations of wild-type decorin core protein used were as indicated (B) Interaction with wild-type decorin in HBS buffer (solid lines) andin HBS buffer containing 15 lM ZnCl2 (dotted lines) Concentrations ... J.S & Termine, J.D (1991) Decorin interacts with fibrillar 20 21 22 23 collagen of embryonic and adult human skin J Struct Biol 106, 82 90 Takahashi, T., Cho, H.-I., Kublin, C.I & Cintron, C (1993)...
... activity of trehalase in slab gels was expressed as enzyme units per mg protein Protein determination was performed by absorbance measurement at 28 0 and 20 5 nm according to the methoddescribedin ... protease inhibitor cocktail (Sigma Chemical Co.) A flow rate of 0.4 mLÆmin)1 was used and the elution was tracked by absorbance at 28 0, 23 5 and 22 0 nm Blue Dextran (20 00 kDa; Sigma) and vitamin B 12 ... that was cleaved with XhoI and NotI and cloned into plasmids pIH-ura and pIH-LEU These are integrative plasmids derived from plasmid pDS472a [20 ], without nmt promoter and ars1 sequences, and with...
... fixed-point formula as applied to the de Rham complex of a variety equipped with the action of a finite group This yields a formula forsome linear combinations of logarithms of periods of the ... variety in terms of derivatives of (partial) Lerch ζ-functions Using the Hurwitz formula andsome combinatorics, we can translate this into Theorems andIn general the fixed-point formula of [13], ... properties of the periods of X are in uenced by the algebraic subvarieties of X; a subvariety of X has a cycle class in the dual of a rational homology space of X and the duals of these cycle classes...
... Ôarms domainÕ of CLR, which contains the binding site of the tetramer C1r2–C1s2 It has been reported that basic residues lysine and arginine in the hinge region are involved in the assembly of C1 ... protein [21 ] andof DNA [22 ] to C1q leads to an activation of Complement On the other hand, the binding of sulfated glycosaminoglycans [23 25 ], of proteoglycan dermatan Reagents and proteins The ... compete with purified C1q* for the binding to DNA When incubation of T4 DNA and C1q* was performed with various amounts of CLR, the analysis of the resulting combed DNA showed that the binding of C1q*...
... to, and inactivating caspase-3, caspase-9 and cytochrome c [29 ,59] Prior to the induction of hsp25, we have shown that MG1 32 promotes a transient increase in the level of eIF2a phosphorylation and ... types, inhibition of the 26 S proteasome induces activation of stress kinases, promotes apoptosis [16,17 ,20 ,25 ,29 31, 38] or potentiates the cleavage of eIF4GI [1, 42, 52] In addition, treatment with ... expression of hsp25 and its increased association with eIF4F Cell stress, including MG1 32 treatment, has been reported to increase the expression of the stress-related proteins, hsp25, aB-crystallin and...
... GST–nephrincyt vector as a template in PCR, the nephrin cDNAs containing amino acids 11 02 1 122 (GST-1 122 ), 11 02 1173 (GST-1173), 11 02 124 5 ( 124 5), and 1167– 125 6 (GST–Cterminus) were created, and subsequently ... in Fig 1B, both podocin and Fyn kinase readily associated with GST–C terminus and GST–nephrincyt CD2AP did not interact with the fusion proteins in the initial experiments, and we therefore investigated ... 27 2 (20 05) 22 8 24 3 ª 20 04 FEBS X L Liu et al Intracellular domain of nephrin phosphatidylinositol 3-kinase that has been reported recently to associate with nephrin [37] We found that PI 3-kinase...
... Schlessinger, J (19 92) The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling Cell 70, 431 4 42 12 Batzer, A.G., Blaikie, P., Nelson, K., Schlessinger, ... FEBS 20 02 Ligand specificities of ErbB-1/ErbB-4 chimeras (Eur J Biochem 26 9) 23 25 contain N-terminal portions of the extracellular region of ErbB-1 ( )24 to 479, )24 to 311 , and )24 to 163) and ... contrast, the replacement of domain III andof domains III and II, resulting in 1144-4 and 1444-4, respectively, greatly decreased the 125 I-labeled EGF binding, indicating that domain III of ErbB-1...