generation and characterization of oligodendrocytes from lineage selectable embryonic stem cells in vitro pdf
Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... doses of SEB both in vitro and in vivo Our lead antibody HuMAb154 was selected for further characterization because of its high affinity and in vitro high potency and was tested in mouse models of ... assess noise, and to normalize injection data All samples were randomly injected for two minutes using the KINJECT command and the dissociation phase of binding was observed for 10 minutes The data ... 2-mercaptoethanol (Invitrogen) in the presence of irradiated CHO feeder cells Cultured B cells were then electrofused with CB-F7 heteromyeloma cells [21] (kind gift from Dr Roland Grunow, Robert Koch-Institute)...
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Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)" docx
... factors, including squamosa promoter binding proteins, MYB, NAC1, homeodomain-leucine zipper protein, auxin response factor, CCAAT-binding protein, scarecrow-like protein, APETELA2 protein and MADS ... across all major lineages of plants, including mosses, gymnosperms, monocots and dicots; for example, AthmiR166, miR159 and miR390 are conserved in all lineages of land plants, including bryophytes, ... protein, binding protein, ELIP, and expansin like protein A, suggesting that wheat miRNAs are involved in a broad range of physiological functions Further analysis indicated that target genes of...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... (VH domain) and three constant domains (CH1, CH2 and CH3 domains) L chains contain a variable domain (VL domain) and a single constant domain (CL domain) The complementarity-determining regions ... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101 Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and ... Genotype from Malaysia and Thailand, Asian Genotype from Vietnam, China, Taiwan, Sri Lanka and the Philippines Australia, East and West Africa, the Pacific and Indian ocean islands, Indian subcontinent...
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Isolation and characterization of allergens from curvularia lunata 1
... of great importance locally, and a better understanding of allergens from this genus would assist in better understanding the allergic reactions and would also aid towards developing SIT against ... generating recombinant proteins In view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and ... obtained data will help in understanding the allergens and allergenicity of Curvularia lunata as well as fungi in general and will assist in development of improved fungal allergy diagnosis and...
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Isolation and characterization of allergens from curvularia lunata 2
... giving rise to the class of Pan Allergens An attempt to identify these IgE binding components was also made by excising the bands corresponding to the IgE binding bands from 1D western blot and ... in order to get the identities and corresponding cDNA sequences (from ESTs) of the identified protein components Band B2 was found to be contaminated with keratin and hence was not included in ... tested The 15kDa band showed the least binding frequency and was only seen in four of the tested species IgE binding patterns of the protein components suggest that the allergenic protein component...
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Isolation and characterization of allergens from curvularia lunata 3
... Transformant colonies having correct allergen insert were inoculated into 5ml of LB broth containing 100µg/ml of ampicillin and incubated at 37°C at 230 rpm overnight From the grown starter culture, ... reading of the working membrane’s buffer in which the test sample was diluted The final reading was taken as the difference of the reading of the test sample and the sum of both; the allergen and ... System (Roche Diagnostics) possessing proofreading activity A 50µl reaction consisted of 0.5µl of each primer (100µM), 5µl of Expand Long Template Buffer 1, 2µl of dNTP (10µM), 3µl of cDNA and...
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Isolation and characterization of allergens from curvularia lunata 4
... useful in standardization of allergen extracts by aiding in proper quantitation of allergens by comparing the strength of reactivity to the source, in order to administer proper amount of allergen ... by skin prick testing To test the in vivo IgE binding of the allergens, skin prick testing was carried out Equimolar mixtures of various allergens in the allergen groups were used A drop of 0.05mg/ml ... protein band (double the expected) size was identified as thioredoxin from the 1D western blots in Chapter Levels of Thioredoxin in the environment (indoor and outdoor) could not be quantified using...
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Isolation and characterization of allergens from curvularia lunata 5
... various fungi (including C.lunata) were developed The IgE binding bands present in C.lunata were excised and subjected to tandem mass spectrometric identification different IgE binding components ... superoxide dismutase, Cyclophilin, heat shock protein 70, Calcium binding protein and Enolase) were identified suggesting these proteins to be expressed in the proteome All of these allergens (except) ... heterogeneous mix of proteins and has varying amounts of antigens The level of allergens may vary from batch to batch Also, the extracts might also be contaminated by allergens from other sources and may...
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EXTRACTION AND CHARACTERIZATION OF CHITIN FROM CRUSTACEANS
... differed from the rest in the intensity and width of the band at 1420 cm -‘ Beran et al.,13 purified chitin from fungi, showing that, in this case, the relative intensity of the bands at 1630 and ... 15min at room temperature Washing Scheme and drying Isolation of chitin Extraction and characterization (a) Demineralization Shell particles were demineralized with N HCl at room temperature and ... stoichiometry, chitin from the former group of samples (the first three in Table 2) and that from prawn in particular, contains the most deacetylated units in its structure Chitin from Fusarium oxysporum...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... in lane 1, runs only marginally above the hA2aR fusion protein and is the main band in lane In this puri®cation, 7% of speci®c ligand binding sites loaded onto the XAC-agarose were detected in ... obtained, namely LysIle-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the mature maltose-binding protein Binding of the fusion protein to the IMAC gel in buffer containing ... Radioligand binding-analyses on E coli and P pastoris membrane preparations, containing the S2 or the S4 somatostatin receptor fusion protein did not reveal speci®c binding of the agonist somatostatin...
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Báo cáo hóa học: "Synthesis and characterization of VO2-based thermochromic thin films for energy-efficient windows" pdf
... the conduction band Since intrinsic VO2 thin film is of n-type, introduction of ion donors cause an inevitable degradation of the transmittance (and resistivity) of the semiconducting low-temperature ... in the VO2 solid solution The decrease in the IR modulation efficiency resulting from doping is mainly due to decrease in the transmittance in the semiconducting state This decrease is explained ... wavelength, in this case at l = 2500 nm The transition temperatures were then estimated by determining the first derivative of both curves of the hysteresis loops (heating and cooling) and considering...
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Báo cáo vật lý: "Synthesis and Characterization of Bismuth Tantalate Binary Materials for Potential Application in Multilayer Ceramic Capacitors (MLCC)" doc
... Pellets of single phase sample were prepared using a stainless steel die measuring mm in diameter Sufficient amount of powder was added, cold pressed uniaxially, and sintered at 1100oC in order to increase ... frequencies in spectroscopic plots of the imaginary components of the impedance (Z'') and electric modulus (M''), as shown in Figure The frequency maxima of Z'' and M'' should be coincident, and the ... frequency region with the increase of measuring temperature; this indicates the presence of polarization process in the dielectric material.15 The broadening of the peaks with increasing temperatures...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Study of Ethical Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington...
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Báo cáo hóa học: " CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation" pptx
... culture and development of multidrug resistance Human hepatoma HepG2 cells were purchased from the Institute of Hematology of Tianjin, Chinese Academy of Medical Sciences (Tianjin, China) To ... MDR1 levels of HepG2 and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 ... the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups...
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Báo cáo khoa học: "Establishment of explants from 200-year-old Quercus petraea in culture" pdf
... cut surface of the segment and at the points from which side branches had arisen Some segments from the young branch also produced shoots from points at which branching or sectioning had not ... established in culture, regardless of age However, the difficulty of characterizing the origin of shoots produced from Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, ... segments of the young and old branches were determined acwere to their tree-ring patterns and found to be 23 and 41 years old, respectively There was cording no replication While still on the tree, in...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... resemble aggregates consisting of preosteoblasts Our finding of a mineralization in microspheres of DAG-treated ESCs seems to share similarity to the formation of bone and dentin in vivo Thus, it appears...
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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf
... triple-reassortant swine influenza virus acquiring a neuraminidase and matrix (M) gene segment from viruses of the Eurasian-avian-like swine virus lineage [5,6] Since these swine viruses have in turn originated ... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells ... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain,...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
... determining a wide array of biological effects from infection of susceptible cells to induction of signal transduction intracellular pathways [63] In particular the interaction between gp120 and ... several cell lineages such as endothelial cells, osteoblasts, adipocytes and smooth muscle cells [37,38] Hence, these cells may be the targets of HIV and/ or viral proteins inducing direct or indirect ... determined using Student’s t test with *p < 0.05 smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... fibrin glue, mesenchymal stem cells and platelet-rich plasma Clin Oral Implants Res (5):579-586 51 Awad HA, Butler DL, Harris MT, et al 2000 ;In vitro characterization of mesenchymal stem cellseeded...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... differentiation of stem cells into well-defined lineages in vitro, by introducing ECM molecules into the culture milieu In addition, cell-ECM interactions may promote the proliferation of these adult stem cells ... Likewise, M-band proteins such as myomesin, M protein and the C-terminus of titin play a significant role in incorporating the myosin thick filaments into regular A bands19-25 Importantly, the coincident ... bodies composed of -actinin, the N-terminus of titin, nebulin, and telethonin contribute to the polarized organization of the thin actin filaments to form I-Z-I bands in developing sarcomeres11,...
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