... their positions. The arrowheads depict the residues important for
the structural core ofthe IL-10 gene. The underlined amino acid residues are the signal sequences ofthe respective genes. The ... analysis investi-
gated. This work will pave the way for further investigation
of the biological function of this gene, and the probability of
the presence of IL-10-related genes in fish, as seen in
mammals.
Acknowledgements
This ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the
carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...
... 1).
Subsite mapping
On the basis ofthe assumptions of Hiromi [30] that
the intrinsic rate of hydrolysis (k
int
) in the productive
complex is independent ofthe length ofthe substrate,
K
m
and ... Laboratory of Microbiology, Wageningen University, the Netherlands
2 Laboratory of Food Chemistry, Wageningen University, the Netherlands
Pectin is a complex polysaccharide present in the cell
wall of ... (1973) Subsite
affinities of glucoamylase: examination ofthe validity of
the subsite theory. Biochim Biophys Acta 302, 362–375.
L. Kluskens et al. An exopolygalacturonase from Thermotoga maritima
FEBS...
... (e.g., the height of a person), while
for others it is only the center of a distribution
(e.g., the weight of a coffee cup). Questions
with no trusted and exact answers were eliminated.
From the ... information for the
requested object, the requested object will receive
the average ofthe extracted values ofthe whole set
of the retrieved comparable objects and the com-
parison step will be essentially ... value for the given
object. During the first stage it is possible that
we directly extract fromthe text a set of values
for the requested object. The bounds processing
step rejects some of these...
... be
collected from each seed word, and then checked
whether each ofthe target terms was included in
the system output. We counted the number of tar-
get terms in the following five cases. The right ... total;
the average number of output terms per input is 12.2
terms. We checked whether each ofthe 610 terms
is a correct related term ofthe original seed term by
hand. The result is shown in the ... term.
3. There is a definition or explanation ofthe term.
4. There are several technical terms that are re-
lated to the term.
We have implemented the checking program of the
first two conditions in the...
... quality
model to estimate the dispersion function, the
accuracy ofthe estimated emissions will depend
on the ability ofthe model to reproduce the
dispersion ofthe pollutants.
Until now, ...
observed at this period ofthe day. From 2 pm to
6 pm, the wind direction is oblique to the street
axis and the wind speed is higher than in the
morning. At these hours ofthe day, tracer
... which, C is the concentration of a particular
pollutant in the street (g/m
3
or mg/m
3
); E is the
emission ofthe pollutant from road traffic in
the street; F is a function describing the
dispersion,...
... the finding that at pH 10 and 75 °C,
the inactivation of b-glucosidase also does not involve
any loss of secondary structure [1].
Conclusions
The stability ofthe b-glucosidase fromthe hyperther-
mophile ... 113
ofthe large volume change associated with the forma-
tion of a free charge [30]. Hence, a reduction in the
number of electrostatic interactions would increase the
pressure stability ofthe ... addi-
tion, the band at 1.0 GPa in Fig. 5C does not
resemble the broad, featureless band typical of an
unfolded protein. Taken together, these observations
suggest that the unfolding at the level of the...
... arrows represent the direction of transcription; the
site ofthe transposon insertion identified in the O35E is denoted
as EN:TN, and the location ofthe deletion replaced by the kanamy-
cin-resistant ... moieties. The ions with m ⁄ z of 1519.6
and 1541.6 are mono- and di-sodiated forms of the
de-O-acylated LOS and the ion with m ⁄ z of 1479.6 is
the anhydrous form ofthe de-O-acylated LOS. The
ion ... de-O-acylated LOSs fromthe wild-type O35E
and the mutant O35Elgt3 were further analyzed by
MALDI-TOF MS (Fig. 4). The mass spectral data of
de-O-acylated LOS from O35E was consistent with the
reported...
... construct expected pay from civilian airlines. Furthermore,
because much ofthe variation ofthe civilian pay profile lies in the probability of
being hired, the number of civilian major airline ... characteristics of military
employment, such as the DOD occupation code). Appended to the accession data
is information about the officer at the time of separation if the officer is no longer
in the service, ... Specification ofthe Pilot Attrition Model
In this section, we discuss the details ofthe development ofthe specific
mathematical form of our pilot attrition model.
Dependent Variable
The dependent...
... positively affected the activity and
stability ofthe enzyme [40]. The effect of copper on
enzyme stability may be related to the inhibitory effect of
the metal on the activity of an extracellular ... to analyse the expression ofthe gene.
Right panel: PCR amplification ofthe cloned
lac1 cDNA using the cycle number obtained
from the left panel. Each value represents the
mean ± SD of three PCR ... as above. The full-
length cDNA of lac1 was then generated by 3Â-RACE
using the primer 5Â-TCTCAACCGTCGACAGCAG
TGTTCGTG-3Â designed fromthe sequence ofthe extreme
5Â end of lac1. The full-length...
... SGTI
is the differing conformation of their binding loops. The
corresponding NOE pattern of SGTI is different from that
of SGCI as well as from that recorded f or SGCI[L30R,
K31M]. The number of observed ... inhibitors.
In the light ofthe solution structures reported here, we
attempt to address the question of taxon-speciđc inhibitory
action fromthe inhibitor side. The binding loops of each of
the molecules ... degree of
stabilization by interactions with the core ofthe molecule.
Although w e only have i ndirect evidence concerning the
¯exibility ofthe binding loop ofthe inhibitors, we suggest
that the...
... and indeed, the site-specific mutagenesis of
K253 to Met led to an inactive protein [23]. In addition,
the alkylation ofthe adjacent M258 inactivated the IDH
from A. vinelandii [26]. These Lys ... that leads to inactivation of the
enzyme and consequent switch ofthe carbon flux from TCA
cycle to glyoxylate cycle [13] [14].
There are two k inds of IDH with different cofactor
dependency, NAD- ... ATP-citrate lyase
[24]. The results demonstrated the heteromeric structure o f
this enzyme and its role in regulating the d irection and flux
of the RTCA cycle. For further understanding ofthe R TCA
cycle,...
... dismutase. Furthermore, we investigated the
binding of hydroxide and imidazole to the ferric enzyme and
of NO to the ferrous enzyme to establish the nature of the
proximal ligand bound to the heme ... the enzyme from GR-1 [4]. However, the existence
of a second high-spin species, which we have designated the
ÔbroadÕ signal, has not been reported previously. The nature
of the multiplicity of ... for the formation of a
complex with hydrogen peroxide (Fig. 1, trace E). The EPR
spectrum ofthe hydrogen peroxide complex, however,
shows a decrease in the high-spin signal ofthe enzyme and
the...
... pK
a2
. The pK
a
value is deter-
mined from a fit ofthe phosphorus chemical shifts of
the without ionic, monoionic and diionic forms of the
phosphate group as a function of pH [33,34]. The
phosphorus ... affects the electrostatic potential and
quite often the conformation ofthe modified protein.
Even in the absence of rearrangement, the change
in the electric field and steric hindrance from a
phosphate ... contribution ofthe R4 repeat to PHF
formation remains to be elucidated, even though the
roles ofthe R2 and R3 repeats in the aggregation of tau
have been reported [22]. Moreover, there is no...
... name”)
The value ofthe :cs feature is the constituent struc-
ture ofthe subcategorisation frame, which lists the
syntactic CF-PSG constituents in sequence. The
value ofthe :gs feature is the ... lexicon extracted from Sec-
tion 23 ofthe WSJ. It was found that the reference
CCG lexicon contained 95.09% ofthe entries in the
test lexicon, while 94.03% ofthe entries in the test
TAG lexicon ... fea-
ture to the generated f-structures, the value of which
is the syntactic category ofthe pred at each level
of embedding. Exploiting this information, the ex-
tracted semantic form for the verb...
... in the amino-acid sequence
of P. hilaris cellulase. Alternatively, the mobility of the
P. hilaris cellulase protein on SDS/PAGE may be different
from those ofthe standard proteins used in the ... to belong to GHF 5. The
activity staining indicated that there were no other CMCase
activities except the GHF 5 cellulase in the larval gut of
P. hilaris. The sensitivity ofthe activity staining ... found in the sequence
(Fig. 3). Genomic PCR experiments with a variety of
different primers designed fromthe cDNA sequence
resulted in the amplification of bands in each case.
Sequencing of these...