distribution characterization of mycobacterial glycolipids and host responses
CHARACTERIZATION OF MYCOBACTERIAL ESTRASES LIPASES USING COMBINED BIOCHEMICAL AND COMPUTATIONAL ENZYMOLOGY
... have been undertaken and the outcome of these studies has opened up new ways and avenues to analyse and characterize a host of diseases as well as providing newer insights and approaches into ... number of mechanisms and pathways to cause and allow subsequent persistence of diseases in human hosts The molecular strategies used by the bacteria to interact with the host can be unique and characteristic ... (Rv1076), lipY (Rv3097c) and are of high functional importance 1.5 Issues and Problems with functional Mycobacterial putative Esterases/Lipases characterization of A literature survey of the studies conducted...
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Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... vant Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, ... cut-off of 3500 Da Standard suppliers were used for all other chemicals Water was deionized and puried using a Millipore system Urea and Gdm Cl stock solutions were prepared gravimetrically and ... 14ị which are similar to Eqns 5, and 7, respectively Here, mmiDHi and mmiDSi are the values of mDHi and mDSi at the ể FEBS 2004 Conformational stability of HPr of S coelicolor (Eur J Biochem 271)...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... kinetic parameters and transport characteristics of Bip-[3H]Pro Results and Discussion Synthesis, chemical characterization and stability of Bip-Pro Figure shows the structure (Fig 1A) and the synthesis ... cells and 10 and 100 lm in SKPT cells) in the presence of increasing concentrations of Bip-Pro (0– 100 lm and 0–50 lm, respectively) The results are Fig Interaction of Bip-Pro with PEPT1 and PEPT2 ... concentrations and at increasing Bip-Pro concentrations The diffusional component of [14C]Gly-Sar uptake, of 8% in Caco-2 cells and of 4% in SKPT cells, measured in the presence of excess of Gly-Sar...
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Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot
... Da and a theoretical pI of 7.65; bcp3 encodes a putative protein of 149 amino acids with a predicted molecular mass of 16 976.42 Da and a theoretical pI of 8.84 Comparison of the sequences of ... measurement of the purple-colored ferrithiocyanate complex developed after the addition of 0.2 mL of 10 mm Fe(NH4)2(SO4)2 and 0.1 mL of 2.5 m KSCN, using H2O2 as a standard The amount of the ferrithiocyanate ... protein of 153 amino acids with a predicted molecular mass of 17 460.12 Da and a theoretical pI of 7.73; bcp4 encodes a putative protein of 156 amino acids with a predicted molecular mass of 17...
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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... samples and Endogenous activity of SF (lanes 6, 14) in the presence of 10 mM viomycin (lane 7) and 0.5 mM tetracycline (lane 15) Ribosomes were incubated with antibiotics before the addition of SF and ... concomitant release of tRNA [5] The activity of SF was found to increase threefold in the presence of TC ribosomes (Fig 4, lane 2; 450 pmol pppGppÆpmol SF)1Æmin)1) and twofold in the presence of reassociated ... formed with twofold excess (A, C, D, E) or equimolar amounts of tRNAMet (B) before addition of threefold excess f of tRNAPhe (D, E) DMS (A, B, E) and kethoxal (C, D) modifications and primer extensions...
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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
... to Erlandsen and Stevens [22] The details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, ... family and a shorter fragment of 253 bp present only in the proband, as well as in his mother who bears the same mutation (Fig 3) Direct sequencing of both cDNA bands conrmed the skipping of the ... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression...
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thermal oxide synthesis and characterization of fe3o4 nanorods and fe2o3 nanowires
... composition of the sample, and the results can be seen in Figure 4(d) It can be clearly identified that the nanorods were composed of Fe and O elements, and the ratio of the number of atoms Fe and O ... of single nanorod with the length of 0.8 μm and width of 0.6 μm under the high multiple When the reaction temperature was increased to 400℃, the samples were a small amount of nanolines 2.3 Characterization ... (c)－(f) and the side SEM image was shown in Figure 1(b) As seen in Figure 1(c) and (e), the shape of nanorods samples were the appearance of rectangular cross-section and with the length range of...
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Báo cáo khoa học: Characterization of inhibitory mechanism and antifungal activity between group-1 and group-2 phytocystatins from taro (Colocasia esculenta) pdf
... two bands corresponding to GST-FL (upper band) and GST (lower band); Nt lane, GST-Nt peptide (upper band) and GST (lower band); Ct lane, only one band (GST-Ct peptide) (B) SDS ⁄ PAGE analysis of ... and Ct peptides is important for inhibitory capacity of the FL peptide, equal amounts of Nt and Ct peptides were mixed and the inhibitory capacity of the mixture was then compared with that of ... development and defense responses to biotic and abiotic stresses [13–15] Although at least two functions have been assigned to phytocystatins, such as regulation of protein turnover and protection of...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... relative elevation of pKa of PLP phosphate in unliganded CcStP and the apparent lack of perturbation of pKa in the CcStP complex with arsenate The pH dependence of the 31P chemical shift of PLP phosphate ... annealing and elongation time of and an annealing temperature of 55 °C We used the following pairs of flanking and mutagenic primers, where Eco91I and XagI restriction sites are underlined and the ... 5108 Fig Characterization of PLP phosphate in wild-type CcStP and the H334G mutant using 31P-NMR (A) Spectra of wild-type CcStP and the H334G mutant acquired at pH 7.25, and with the number of recorded...
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Báo cáo khoa học: Characterization of the lipopolysaccharide and b-glucan of the fish pathogen Francisella victoria ppt
... gain an understanding of the molecular basis of their biological properties, the host specificity and diversity of members of this important bacterial genus, as well as the nature of its pathogenicity ... configuration of the monosaccharides, which turned out to be l for Rha and Fuc, and d for QuiN, Qui3N, Glc, Man, and GlcN Configurations of Fuc4N and Qui4N have not been determined because of unclear ... H-3 of the Kdo C and H-6 of the Kdo D residues indicated the attachment of Kdo D in a-configuration to O-4 of Kdo C All glycosidic linkages were identified on the basis of transglycosidic NOE and...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... start of the oraS gene and a BamHI site into the end of the oraE gene Genomic DNA was purified from C sticklandii by phenol/chloroform extraction methods [4] The coding regions for the S and E ... measuring the apparent Km value of D-ornithine at 25, 50, 100, 200 and 400 lM of the inhibitor For the measurement of the activity of the substrate analogues, an HPLC and NMR-based method was developed ... in various concentrations of PLP, and the binding of coenzyme was measured PLP was bound with an apparent Kd of 227 ± 41 nM (Fig 3) Fig UV–visible spectrum of wild-type and mutant D-ornithine aminomutase...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... Phenotype of MDSC shows CD33+ and CD11b+ subsets to be both HLA-DRlow and Lineage- Further characterization of CD33+ and CD11b+ MDSC subsets was performed using a wide range of proposed MDSC and mature ... Page 12 of 20 Figure Morphology, phenotype, and function of CD11b+ and CD33+ MDSC subsets A, Morphology of human CD33+ and CD11b+ MDSC subsets isolated after tumor cell line co-culture and normal ... GraphPad Prism software (La Jolla, CA) with a significance level of 0.05 Graphs and figures were produced using GraphPad Prism, Microsoft Excel, and Adobe Illustrator and Photoshop software (San...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... &EAB555/R: PCR conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni...
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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc
... against ICP27, and a strong response to pool of ICP0, pools 3/4 and 5/6 of ICP4, VP22 and gD The responses to pool of ICP0 and pool 1/2 of ICP4 were not considered positives as these responses were ... small numbers of antigens, whereas the CD4 responses recognize a broad range of antigens CD8 responses Definition of CD8 responses Eight of the 17 infected subjects had positive CD8 responses with ... http://www.virologyj.com/content/3/1/54 200 100 Samples Figure T-cells Comparison of responses by bound and free CD4 and CD8 Comparison of responses by bound and free CD4 and CD8 T-cells PBMC samples were treated with magnetic...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C ... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... &EAB555/R: PCR conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni...
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báo cáo hóa học:" Fabrication and characterization of well-aligned and ultra-sharp silicon nanotip array" ppt
... Fabrication and characterization of well-aligned and ultra-sharp silicon nanotip array Chi-Chang Wu*1,2, Keng-Liang Ou1,2, and Ching-Li Tseng1 Graduate Institute of Biomedical Materials and Tissue ... 1,000 V and measured the emission current Results and discussion The progress of transformation of the tips and photoresist at different etching time is displayed in Table From the eagle-view and ... microscopy, and scanning tunneling microscopy are intensively investigated [4] The efficiency of these techniques strongly depends on the characterization of the tip For instance, a high-brightness and...
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Báo cáo hóa học: " Synthesis and Characterization of ZnO Nanorods and Nanodisks from Zinc Chloride Aqueous Solution" pptx
... morphologies of the samples prepared in aqueous solutions of equimolar of ZnCl2–HMT mixture with and without 50 vol.% EG additive The initial concentrations of Zn2? were 0.01 M, 0.05 M, and 0.1 M, ... respectively The morphology of the product changed greatly depending on EG additive and concentration of Zn2? In the absence of EG, at such low initial ZnCl2– HMT concentration of 0.01 M, the product ... dispersibility and glutinosity It was also found that in the absence of HMT in 0.1 M ZnCl2 solution, only small amount of products with nonhomogeneous morphologies consisted of spherical and rod-like...
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Báo cáo sinh học: "Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists." pps
... ‘chemical genetic’ approach of identifying and studying the mechanism of action of small-molecule agonists (and antagonists), we hoped to uncover some of the complexities of the Hh-signaling system ... activation with a Hh-protein ligand has therapeutic value [49,50] On the basis of our current understanding of these models and the specific mechanism of action of the Hh agonists, we predict ... concentrations of Hh-Ag 1.3 (n = explants) Error bars represent standard deviations (c-n) Confocal images of representative explants cultured in the presence of different concentrations of the agonist and...
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Báo cáo khoa học: "Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex" docx
... virulence of the virus is much less than that of them [6, 13] Along with severe disease of leukocyte and lymphocyte counts, each number of MHC class II, CD1+, CD2+, CD4+, CD8+, CD4+CD8+ and surface ... humoral immune responses of T-1 and T-2 were greater than those of T-3 or control group A report supports the result of our study, suggesting that in pigs the Ig-containing cells isotypes of the various ... Jeong, and B K Park Enhancement of host immune responses of sows using a non-specific immunomodulator p.22 Proceed 15th International Pig Veterinary Society Congress, Birmingham, England, 5-9...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25