... units in proteins, retaining their structure and usually their function even when being part of proteins with different domain architectures [24] Information about recognized protein domains is ... for the ‘supergroup’ Rhizaria [32]), for the presence ofprotein domains, as defined by Pfam [25] (Figure 1; Additional file 1) The number of distinct protein domains varies from roughly 2,000 in ... The evolution of most eukaryotic groups is dominated byprotein domain losses and not by domain gains While the number of distinct domains found in extant species shows a weakly growing trend (with...
... responsible for the ubiquitination of proteins destined for degradation bythe proteasome and the enhanced CD4 turnover in presence of Vpu Indeed, further lines of evidence for an involvement ofthe Ub-proteasome ... mediated by Vpu involves multiple steps that are initiated bythe direct physical binding of Vpu to the cytoplasmic tail of CD4 inthe ER [21] Although the binding of Vpu to CD4 is necessary to induce ... the ER in presence of Vpu On the basis of these novel findings, a systematic analysis ofthe role of cytosolic serine, threonine, cysteine and lysine residues in Vpu-mediated CD4 ubiquitination...
... the expression of an oncogene carried bythe virus, by insertional activation of host cell oncogenes, or by inactivation of host cell tumor suppressor proteins The Env proteinof JSRV can transform ... other than the lung in JSRVinfected sheep, but we wanted to see if JSRV Env could induce tumors in other tissues in mice Tail vein injection ofthe AAV6 vector encoding JSRV Env resulted inthe ... mouse tumors induced by administration of an AAV6 vector that expresses only the ENTV Env protein [12] Mab staining of tumors from sheep We next tested the Mab for staining of lung tumors in sheep...
... Standard Methods for the Examintion of Water would be to perform an inter-laboratory validation study ofthe proposed methodDeterminationof EDTA in river water samples For the application of this ... upstream ofthe sampling site of Kamo River, EDTA was not detected in Kamo River sample On the other hand, in Shikoku region, EDTA was not observed inthe river water samples The concentration of EDTA ... analytical method to the Japanese Standard Methods for the Examination of Water Occurrence of EDTA in river water samples from three regions of Japan was examined EDTA was detected in ten of thirteen...
... analysis of purified fusion proteins consisting of maltose-binding protein (MBP) linked at its C-terminus to one ofthe NBDs of SUR2 (MBP-NBD fusion proteins) Figure 1B,C shows SDS/PAGE analysis of ... nucleotide-binding sites (site and site 2) that comprise the Walker A and Walker B motifs of one NBD and the linker domain ofthe other Inthe absence of Mg2+, there is little difference in ATP block of ... Wet et al domains (TMDs) and two intracellular nucleotide-binding domains (NBDs) It is thought that, as in other ATP-binding cassette (ABC) proteins [4], the NBDs of SUR associate in a head-to-tail...
... affinity by carbamylcholine The experiment was performed inthe presence of mM carbamylcholine Dotted line, inthe presence of 0.2 mM atropine; solid line, inthe absence of atropine atropine (Fig ... carbamylcholine) [25] These results indicate that M2 inthe GOA-1 fusion protein is functional, and the ligand-binding properties agree with that ofthe Gai1 fusion protein Activation of GOA-1 by muscarinic ... expressing the M2::Gai1 fusion protein [25] Here, similar binding properties of GDP and GTPcS were observed inthe membrane preparations expressing myc-M2(ND)I3del::GOA-1 The binding of GTPcS was increased...
... rates of hydrolysis in lmolÆmin)1Æmg protein) 1 (B) Dependence ofthe uncoupler-induced ATPase activity (j) on the time separating dilution of SMPIF1 in succinate-containing medium and addition of ... (compare terminal parts of curves b and c) Inthe experiment shown in Fig curve d, inhibition by IF1 was studied using SMP inthe presence of malonate, which fully inhibited the respiratory chain (control ... on the pH ofthe medium containing no SMP or SMP treated with excess of oligomycin First-order kinetics of inhibition by IF1 [39] was fitted to the equation: Fig Time-course of ATP hydrolysis in...
... decrease ofthe Km for threonine from 70 mm inthe absence to mm inthe presence of this cofactor [20] At the same time, binding of AMP to E coli threonine dehydratase results inthe association ofthe ... Characterization of SR activation bythe PDZ4-PDZ5-PDZ6GAP2-PDZ7-end module of GRIP D-Serine synthesis by recombinant SR inthe presence of increasing molar ratios of (A) the recombinant PDZ6 domain or ... Next, we decided to analyse the binding ofthe PDZ6 domain of GRIP to brain SR using recombinant proteins We expressed recombinant brain SR in Escherichia coli using the pCWori vector as previously...
... not-forprofit organizations statement of changes in net position A financial statement required of federal agencies which reconciles the beginning and ending net position ofthe agency using the accrual ... business income of otherwise tax-exempt not-for-profit entities Determined by relationship to exempt purpose and other criteria accounting, the shifting of resources from one category to another ... (GASB), 17 Other Financing Sources, 61 accounts, elimination, 229 uses-transfers, 99 Other financing uses, classification, 61 Other Financing Uses, Transfers Out /In, 61 Other not-for-profit organizations,...
... sustained bythe increased survival rate in 4-wks feeding group than 2-wks Similar results was reported by other researchers with the direct intraperitoneal injection of L casei 9018 against the ... 3) These strains were selected for further study in vivo Increased CD8+ T cells in two color analysis of flow cytometry The change of T cell subsets was observed after oral administration of ... LABs in adjusting feeding condition of experimental groups To evaluate the antitumor effects, F9 teratocarcinoma cells (1 × 106 cells/ mouse) were inoculated i.p at the day of 14th The change of...
... shorten the duration of administration The bolus administration of liquid nutrition induces gastroesophageal reflux Hence, in order to prevent the reflux, continuous administration is required [9] The ... ofthe nutrition we used for this patient recommends that the administration ofthe semi-solid nutrition be done in 15 minutes Administration should be carried out in a sitting position to minimize ... Page of accompanying images A copy ofthe written consent is available for review bythe Editor -in- Chief of this journal Acknowledgements This study was supported by a grant -in- aid from the Osaka...
... evaluate the effects of EM703 on the inflammatory phase, we investigated bleomycin-induced changes inthe cell populations in BAL fluid on day after bleomycin injection The increase inthe number of ... down-regulation of Smad3 inthe early stage of inflammation and during the reparative phase was in contrast to the expression of collagen [44] In this study, since the decrease inthe expression ofSmad3 ... anti-fibrotic effects of EM703 inthe attenuation of bleomycin-induced pulmonary fibrosis Although there is a room for further investigation ofthe mechanism of EM703 inhibition of bleomycin-induced lung...
... the main errors inthe use of to-infinitives made bythe students in grade 11 at Bac Kien Xuong High School To find out the causes ofthe errors inthe use of to-infinitives bythe students in grade ... Errors inthe use of to-infinitives % Errors inthe use of to infinitive as a subject complement 68.52 Errors inthe use of to-infinitive as a subject 62.36 Errors inthe use of to infinitive ... study of common errors inthe use of to-infinitives I.2 To-infinitive I.2.1 Definition of to-infinitive To-infinitive is an infinitive with to Kam Chuan Aik and Kam Kai Hui (1992) define an infinitive...
... work - by accident; by air; by bus; by bicycle; by car; by chance; by design; by mistake; by plane; by train; by surprise - in bed; in class; in danger; in difficulty; in hospital; in love; in need; ... errors inthe use of zero article made bythe students in grade 11 at Vu Tien High School inthe academic year of 2013 2014 To find out the causes ofthe errors inthe use of zero article made bythe ... better to distinguish the definite article the and the indefinite article “a, an” and the zero article .The teacher should explain the cases where the definite article the or the indefinite article...
... stirring speed because ofthe instability of organic solvent inthe hollow fiber Our purpose is to improve the sensitivity of LPME and address the issue ofthe instability ofthe solvent drop in ... first introduced in segmented-flow determination[ 1] It is based on the injection of a liquid sample into a moving, nonsegmented continuous carrier stream of a suitable liquid Then the injected ... extraction and at the same time as a syringe for direct injection ofthe extract into a GC or GC-MS for analysis The main feature of this method was the use of smaller amounts ofthe organic solvent...
... category Then a survey was conducted with the aim of testing the students’ understandings ofthe wh-questions and finding their common errors inthe use of whquestions The outcome ofthe survey ... 4) Errors inthe use of how much and how many 5) Errors inthe use of main verb in wh-questions 6) Errors inthe use of what and how 18 7) Errors inthe use of how as a greeting rather than a ... 28.09% Errors inthe use of main verb in wh-questions 35.14% Errors inthe use of what and how 22% 20 Errors inthe use of how as a greeting rather than a question 11.33% Errors in answering questions...
... all? The difficulty ofthe quest for identity, notably of naming what you consist of inside, is contrasted with the easiness of naming objects in our surrounding at the very beginning ofthe novel ... Lesser inThe Book of Illusions insinuating failure The influence ofthe environment on the protagonists‟ actions should not be missed in studying their identity, for the construction of identity ... which the present thesis attempts to examine His protagonists often find themselves on the brink of existential precipice, uncertain about the status of their own identity, balancing They start the...
... by ascorbic acid methodIn addition to above regular sampling for thedeterminationofthe seasonal fluctuation of river water and treated water, we determined the daily fluctuation of them by ... that ofthe other parameters, which was probably due to excess aerobic condition inthe MSL systems shown in ORP values near 400 mV Open top ofthe MSL systems allowed air enter the inside ofthe ... on the surface of zeolite With regards to the removal rate ofthe pollutants in Table 2, the differences among the MSL systems were small considering with the difference of hydraulic loading...