derivation maintenance and characterization of rat embryonic stem cells in vitro pdf
Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain ... counterpart of murine EC cells for studying human development and differentiation in vitro (Andrews 2002) hEC cell lines are capable of multi-lineage differentiation in vitro but being of tumor origin ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points All ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We observed...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration ... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... importance of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review,...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Foundation, and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is ... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... regenerative medicine, I investigated the ability of two cell lines– hES2 and hES3– to generate derivatives of mesoderm and endoderm in vitro Differentiation into these particular lineages was of interest ... studies detailing the derivation and maintenance of induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult ... manufacturing practice) guidelines Even with derivation of qualified lines and defined culture methods, the recurring challenges of directing the differentiation of hESCs to generate cell types in numbers...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells ... differentiation (Wu and Belasco, 2005) In C elegans, Lin4 binds imperfectly to complementary sites in the 3'-UTR and inhibits translation of target mRNAs, including Lin28, Hbl-1, and Lin14 (Lin et al.,...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse ... GFAP and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells ... Blasticidin and (B) Zeocin that E14 and AB2.2 could not survive in µg/mL Blasticidin concentration, while E14 did not survive in 250 µg/mL Zeocin selection and AB2.2 in any concentration of Zeocin...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... alterations 128 trained in procedures and protocols designed to minimize low temperature exposure This would include quick handling outside the incubator, as well as the use of pre-warmed and ... pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction ... layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington,...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... binding and nuclear localization), which is shared by all FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... owing to its high thermostability and insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig 1), and...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... expression of the 14-kDa phosphohistidine phosphatase and an endosomal protein may be of interest in the light of the recently described histidine phosphorylation of annexin I from a membrane preparation ... worth noting that annexin I shows endosomal binding in live HeLa cells [35] In conclusion, the native substrates of the 14-kDa phosphohistidine phosphatase described in the present work remain to ... glycoprotein from developing rat intestinal epithelial cells J Biol Chem 270, 1583–1588 33 Wilson, J.M & Colton, T (1997) Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized...
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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx
... optimal imidazole concentrations needed to remove contaminating proteins in the washing step and to elute the recombinant enzyme in the elution step were determined in preliminary experiments Fractions ... 7.0 and were run at Expression in yeast and purification of recombinant BNSEH1 The BNSEH1 was expressed intracellularly in yeast cells at high levels ( £ 10% of the total protein and routinely ... laurate [20] and up-regulated palmitate, stearate and c-linolenate (x-6) B napus lines [21] Several genes encoding sEH exist in potato, soybean and Arabidopsis [11,13,22] and apparently also in...
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báo cáo hóa học:" Identification and characterization of a spontaneous ovarian carcinoma in Lewis rats" docx
... Breeding Laboratories, Inc., Wilmington, MA) were kept in sterile micro-isolator cages and fed food and water ad libitum The institutional guidelines of Johns Hopkins University concerning the ... flasks (Corning Flask 3056, Corning Inc., Corning NY) Cells used for experiments were low passage and maintained in culture for one to three months The doubling time of the cell line was measured ... was extracted and purified from cell lysates of 1-5 × 10 in vitro FNAR tumor cells and the REH cell line of normal rat endothelial cells, as a control, with 500 μl Trizol reagent (Invitrogen, Carlsbad,...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... stained positively (arrow) (e) Intense staining of individual cells in the lining layer of a typical OA synovium (f) An area of an OA synovium demonstrates a lining layer completely devoid of ... c19orf10 staining (g) Intense staining of a hyperplastic RA synovial lining cell layer This staining was typical of most areas of RA synovium where the lining was hyperplastic (h) An area of an RA ... time -of- flight mass spectrometer [10], and proteins were identified by single MS (peptide mass fingerprinting) using ProFound [11] and by tandem mass spectrometry (MS/ MS) using Tandem search engine...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types ... prominent in the centre of the sphere, as demonstrated in histological sections stained with Figure Micromasses consisting of embryonic stem cells were cultured with or without DAG and stained ... effect of fibronectin and laminin on cell retention and rate of osteogenic expression Cell Transplant 1992, 1:23-32 24 Meyer U, Meyer T, Jones DB: Attachment kinetics, proliferation rates and vinculin...
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design, synthesis, and characterization of polymeric materials for uses in energy storage applications
... of the growing polymer chain Upon transfer of the active site to another polymer chain, branching sites can arise causing a change in the morphology of the final polymer On the other hand, if the ... having to with the relative stabilities of the cyclic monomer (ring-strain) and linear polymer structure Typical initiation in ROP occurs via cationic or anionic means by the use of ionic initiators ... numbers of other synthetic polymers were developed and commercialized in response to the growing need for new materials in the automotive and aerospace industries Some of these new materials include...
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