derivation and culture of mouse trophoblast stem cells in vitro pdf

Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...

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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells

Ngày tải lên : 27/11/2015, 11:25

... humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells (mESC) ... GFAP and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells ... silencing of these binding targets, as a result of the combinatorial interactions of Oct-Sox-Nanog binding, has yet to be definitively shown (Remenyi et al., 2004) This thesis explores the roles of...

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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf

Ngày tải lên : 12/08/2014, 11:22

... functions of individual candidates in influenza pathogenesis will require further investigation Lack of the control of transcriptional suppression by zinc finger proteins in pdmH1N1 infected cells ... nine of the genes down-regulated in response to seasonal H1N1 influenza virus (but not pdmH1N1) encode zinc finger proteins, including ZNF175 ZNF175 contains 13 zinc fingers and a KRAB domain, ... differences in regulation of ten zinc finger proteins and seven small nucleolar RNAs in host responses to pdmH1N1 and seasonal H1N1 influenza virus The role of these proteins in influenza pathogenesis...

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Tài liệu Chronic Disease, Functional Status and Quality Of Life Among The Elderly In Singapore pdf

Ngày tải lên : 14/02/2014, 06:20

... quality of life In the face of impaired physical and mental functioning, perceived social handicap stands in the way of realizing quality of life Success in improving physical and social functioning ... quality of geriatric care, assisted living and long-term care, designs for housing, built environment and transportation, and promotion of ‘active ageing’ Population ageing in Singapore is rapid and ... quality of life (healthy ageing) Multi-disciplinary collaboration of research aimed at understanding the ageing and health transition of Singaporean older adults Goal to help formulate strategies of...

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Evaluation and Preparation of the Infertile Couple for In Vitro Fertilization pptx

Ngày tải lên : 05/08/2014, 16:20

... As insulin is one of the main factors that stimulate vascular endothelial growth factor production by luteinized granulosa cells, and metformin decreases ovarian response and circulating insulin ... containing oocytes, and aspirating needles and pipettes should be changed Two randomized studies have shown that a 3–6-mo course of GnRH agonist leading directly into IVF is associated with an increased ... Determination of the efﬁciency of controlled ovarian hyperstimulation in the gonadotropin-releasing hormone agonist-suppression cycle using the initial follicle count during gonadotropin stimulation...

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Báo cáo khoa học: " The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro" pdf

Ngày tải lên : 09/08/2014, 10:20

... than HePC in preclinical investigations [29,51] Intriguingly, in our in vitro study ErPC3 was a more effective inducer of apoptosis in T98G cells than ErPC when given alone and in combination with ... contributes to increased efficacy of the combination Increased induction of apoptosis contributes to increased efficacy of the combination (A, B) T98G cells were treated with a single dose of 10 Gy and ... feasibility and tolerability of oral administration of the drug alone and in combination with radiotherapy in patients with advanced solid tumors Oral administration of Perifosine is safe and mainly...

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Báo cáo khoa học: " Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by Nacylhomoserine lactone quorum sensing signals" ppt

Ngày tải lên : 12/08/2014, 18:22

... epithelial cells and the extracellular matrix proteins fibronectin and laminin [9], and Rck has recently also been reported to mediate a zipper-like internalization of S Enteritidis into cells in vitro ... number of cells per well Cell invasion studies Over night cultures were diluted 1:100 in mL LB and subcultured for hours in 37°C Then the cultures were ten fold diluted twice, first in MEM and subsequently ... used in experiments Cell lines, culture conditions and buffers The cell line HEp-2 ATCC CCL-23 (LGC Standards, Middlesex, UK) was used in the invasion experiments The cells were grown in Minimum...

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Báo cáo hóa học: " Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro" pdf

Ngày tải lên : 21/06/2014, 08:20

... at 37°C in a humidified atmosphere containing 5% CO2, while μg/mL doxorubicin was contained in the culture to maintain K562/AO2 cells drug resistance in its daily culture The inhibition of cell ... K562/AO2 cells than that of K562 cells, as shown in Figure 4a and 4b Interestingly, Page of the presence of PLA/Au nanocomposites could efficiently facilitate the drug uptake of DNR into the ... leukemia cells and lead to the much higher intracellular drug concentration in the target cells, resulting in the remarkable enhancement of the intracellular fluorescence of drug-resistant leukemia cells...

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Identification of oct4 and sox2 targets in mouse embryonic stem cells

Ngày tải lên : 11/09/2015, 16:03

... genome binding sites of Oct4 and Sox2 Thousands of novel Oct4 and Sox2 binding sites were identified, mapping to genes implicated in important cellular functions including maintenance of pluripotency ... Oct4 and Sox2, which are two key transcription factors that are essential in maintaining pluripotency of ESC In order to gain a deeper understanding of the functions of Oct4 and Sox2 in governing ... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Ngày tải lên : 16/09/2015, 15:55

... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain ... counterpart of murine EC cells for studying human development and differentiation in vitro (Andrews 2002) hEC cell lines are capable of multi-lineage differentiation in vitro but being of tumor origin ... L-glutamine, 1X penicillin–streptomycin, 1X nonessential amino acids (Invitrogen), 1X insulin-transferrin-selenium G supplement (Invitrogen) and mM β-mercaptoethanol (Invitrogen) For the derivation and...

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

Ngày tải lên : 18/02/2014, 04:20

... femurs of 80 g rats, plated in IMDM supplemented with 15% heat-inactivated fetal bovine serum and 100 UÆmL)1 penicillin ⁄ streptomycin and incubated at 37 °C in a humidiﬁed tissue culture incubator ... washes and incubation with the nuclear stain 4¢,6-diamidino-2-phenylindone for 20 min, the MSCs were washed in NaCl ⁄ Pi for 10 and mounted in gelvatol for microscopic imaging Protein extraction and ... stimulatory signal in order to secrete IL-1b and TNF-a Hypoxia ⁄ SD induces the transcription and secretion of IL-10 Because of the lack of secretion of the in ammatory cytokines IL-1b and TNF-a from...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... nature and extent of covalent histone modiﬁcations and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... can be detected in HS3, HS2, and the bmajor-globin gene, but not in HS4, or in between HS2 and HS3 The transcripts originating in between HS4 and HS3 are strand-speciﬁc proceeding unidirectional...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...

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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

Ngày tải lên : 18/06/2014, 16:20

... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...

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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Ngày tải lên : 07/08/2014, 20:23

... was loaded into a 96-well plate and examined using a luminometer (Victor3; PerkinElmer, Finland) and a fluorescent plate reader with excitation and emission wavelengths of 485 nm and 535 nm, ... We determined the H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed ... testis and epididymis In vivo and in vitro approaches Reprod Biol 2006, (Suppl 1), 43-58 Bonvin C, Guillon A, van Bemmelen MX, Gerwins P, Johnson GL, Widmann C Role of the amino-terminal domains of...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... B and C: H&E stain, D-G: Luxol fast blue and cresyl violet stain, H, H1, H2 and H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence ... 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury site, proximal and distal margins) For group ASC, × 10 of prepared cells...

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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx

Ngày tải lên : 08/08/2014, 17:20

... studies involving BrdU labeling for studying neurogenesis BrdU is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle and is used for birthdating and monitoring ... in degenerating regions of the CNS [65] Some of the data observed by mean of immunohistochemistry for cell cycle proteins and BrdU labeling in the brains of AD patients and in animal models of ... progenitor and stem cells have been isolated and characterized in vitro, from various regions of the adult mammalian CNS, including the spinal cord [16] This suggests that neural progenitor and stem cells...

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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Ngày tải lên : 09/08/2014, 10:20

... cells, increased in vivo reconstitution of hematopoietic lineages, and Wnt3a-specific maintenance of skin and intestinal stem cell populations [18] Because stem cells may share signaling mechanisms ... neighboring cells [21] FGF2 maintains the stem state of MSCs from a variety of species by prolonging their viability in culture [16], sometimes in a cell-autonomous fashion [17] This is reminiscent of ... promoting MSC adipogenesis while repressing osteogenesis [52] The binding of PPARγ to various ligands, including longchain fatty acids and thiazolidinedione compounds, induces the transactivation and...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant adenoviral ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or ... × 106 cell/mL in serum-free DMEM containing mM pyruvate, 1% ITS + Premix (insulin, transferrin and selenous acid containing culture supplement), 37.5 mg/mL ascorbate2-phosphate and 10-7 M dexamethasone...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... differentiation with increases in insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response ... effects of adding ECM such as FN and LAM on the expression of Insulin and Glucose transporter (Glut2) genes and proinsulin and insulin protein levels We further clarified the underlying mechanism...

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