... induces genetic and epigenetic instability and phenotype changes in prostatecancer cells BMC Biol 2010, 8:6 24 Diaw L, Woodson K, Gillespie JW: Prostatecancer epigenetics: a review on gene regulation ... (GSTP1) gene Epimethylation of the GSTP1 gene is absent in normal prostate tissue and present in 6.4% of proliferative inflammatory atrophy, which is the precursor lesion ofprostatecancer GSTP1 ... tumor invasion and metastasis, is decreased or absent in many cancers and is predictive of tumor progression and poor patient outcome In prostate cancer, decreased expressionof E-cadherin correlates...
... other metabolic events in prostatecancer [14] Taken together, a combination ofgene amplification and androgen stimulation may cause overexpression of TPD52 in prostatecancer TPD52 undergoes post-translational ... protein signature ofprostatecancer biopsies revealed the overexpression of TPD52 in cancer patient material [22] To date, the main physiological role of TPD52 in prostatecancer progression ... cells [34,35] The expressionof murine TPD52 in NIH3T3 cells induces the expressionof several genes involved in the promotion of metastasis and the genes responsible for prevention of metastasis...
... copy number changes andgeneexpression profiles in a cohort of AA and CA PCa patients using BAC-based array comparative genomic hybridization (aCGH), oligo-based aCGH, andgeneexpression array ... reported as common areas of genomic gain in prostatecancer In one series of 18 prostatecancer cell lines and xenografts, 39% of samples had copy number gain at 5p15.33 and 39% had gains at 16p12.2-p11.2[29] ... finding of copy number gains at 16p11.2 and overexpression of RABEP2 in this previous study of PCa cell lines and in our current study of human PCa tissues is reassuring of the validity of the...
... localizes in the nucleus and binds to and regulates basal expression via HSEs of target genes [26] Deletion of the HSF gene is lethal to cells, even at physiological temperatures The genes targeted by ... levels of HSF1 and MTA1 (a component of the NuRD complex containing HDAC1 and HDAC2) proteins and to binding of HSF1 to MTA1 [78] The complex is recruited to the promoters of estrogen-responsive genes ... Sakurai and Y Enoki SWI ⁄ SNF complexes are recruited to both the promoter and coding regions, and histones are acetylated and displaced [56–58] However, the presence of SAGA and SWI ⁄ SNF, and a...
... 36.6 We next determined geneexpression in different adipose depots in response to PM2.5 exposure, in terms of the expressionof BAT-specific and WAT-specific gene profiles by real-time PCR analysis ... differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the geneexpressionof Dpt in BAT or WAT Xu et al Particle and Fibre Toxicology 2011, 8:20 http://www.particleandfibretoxicology.com/content/8/1/20 ... targeted disruption of the Dio2 gene have cold-induced overexpression of the uncoupling protein gene but fail to increase brown adipose tissue lipogenesis and adaptive thermogenesis Diabetes 2004,...
... MEF2 Second, overexpression of a-actinin-4 decreases the interaction of MEF2A and HDAC7 Third, knockdown of a-actinin-4 decreases expressionof TAF55 Fourth, MEF2C, a-actinin-4 and HDAC7 associate ... inhibition of ARA54-enhanced androgen receptor transactivation andprostatecancer cell growth Mol Endocrinol 21, 343–358 103 Lee YH & Stallcup MR (2006) Interplay of Fli-I and FLAP1 for regulation of ... activities of myocardin and MRTF-A and -B, can completely block the ability of STARS to induce SRF-dependent transcription in NIH 3T3, COS1 and 293T cells However, STARS does not alter the level of expression...
... differences in expressionof these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, the expression levels of let-7b and let-7i ... miRNA expressionGeneexpression profiling We assessed global hES geneexpression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and ... gene pairs Correlation coefficients of miRNA-target gene pairs The expressionof miR-302c and miR-502b and their predicted target genes was analyzed by correlation analysis The distribution of...
... Previously, analysis of NS3/4A-mediated inhibition of IFN geneexpression has been restricted to IFN-β gene To further analyze whether the expressionof other type I IFN Page of 13 (page number ... n ei pr ot co re F pc DN A3 Figure HCV NS2 and NS3/4A inhibit IFN-β geneexpression HCV NS2 and NS3/4A inhibit IFN-β geneexpression (A) The effect of expressed 11 HCV polypeptides on IFN-β promoter ... similar mechanism to inhibit IFN geneexpression Cells were transfected with Flag-Cardif and increasing amounts of HCV NS2, NS3/4A and NS3/4A-S139A expression con- Page of 13 (page number not for citation...
... development of manifestations of lupus General aspects of Ig variable region (IgV) gene usage Autoantibodies are features of most systemic autoimmune diseases, including pSS The production and persistence ... distribution of B cells and their productive IgV gene rearrangements can be influenced by a variety of selective events during development and subsequent antigenic stimulation because of the nature of ... than other regions of the VH molecule Evidence of clonal expansions The major salivary glands are known to be the site of preferential B cell expansions and in some cases of lymphoproliferation in...
... et al.: Expression profiles of non-small cell lung cancers on cDNA microarrays: Identification of genes for prediction of lymphnode metastasis and snsitivity to anti -cancer drugs Oncogene 2003, ... induce these genes in group II The results of the study suggest that a combination of histopathology and gene- expression profiling is a useful tool for diagnostic and prognostic studies of early ... histopathogensis of synovitis in early RA Different expression profiles of the several candidate genes may provide useful information for future studies of the diagnosis and prognosis of early RA...
... http://www.ro-journal.com/content/5/1/108 Page of 12 Figure Anti-neoplastic effects of ErPC3 and ionizing radiation on prostatecancer cells The prostatecancer cell lines PC3, DU145, and LNCaP, were irradiated ... down-regulation of p-Akt may be required for the anti-neoplastic action of small molecule inhibitors of the PI3K/Akt pathway in prostatecancer cells Combined effects of ErPC3 and ionizing radiation in prostate ... to the design of the study VJ performed conception and design of the study and substantially contributed to interpretation of data, drafting and critical revision of the manuscript and final approval...
... standard deviation >0.7) In comparing the geneexpressionof the congenic strains with that of the control strains, the standard analysis elicited gene lists of 2,435 differentially expressed genes ... PCR validation ofgeneexpression data Genes were selected from the gene lists of the standard and alternative analyses in order to verify the microarray results using QPCR Only genes with FDR ... data are robust and provide clear differences between the strains Geneexpression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the geneexpression profiling has been...
... of P63 + cancer cells in the metastatic loci and generation of prostate- like glandular structures Our data support the prostatecancer stem cell characteristics observed in previous studies and, ... loss -of- function mutations has been reported in some androgen-refractory prostate cancers [13,41] Therefore, both AR + and AR - cells were capable of forming androgen-independent prostatecancer ... Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development ofprostatecancer Eur Urol 1996, 30:201-205 Collins AT, Maitland NJ: Prostate...
... List of primers used for real-time qRT-PCR of shoot and berry geneexpression Primers were generated for realtime qRT-PCR of genes for comparison with microarray and EST frequency results Gene ... been generated and analyzed to identify geneexpression related to a wide range of processes including berry development in wine grape [30,45] and in table grape [46], tissue-specific geneexpression ... number of times the transcript ofgene xi is observed in relation to the total number of random observations of all genes, (xi / ∑x), any ESTs from a single clone sequenced from both the 5’ and...
... identification and characterisation of genes is difficult EST generation supplements the lack of genome data by providing transcript specific information and excluding non-coding regions of the genome ... Quantitative geneexpression analysis Expression analyses of nine genes were conducted at 0, 3, 6, 12, 24 and 48 hours post infection (hpi) to validate if the pyrosequencing data reflected their geneexpression ... pyrosequencing data andgeneexpression profiles of defence-related genes Sequence ID GenBank accession number cDNA Library Max qRT-PCR expression Similarities between 454 and qRTPCR Thaumatin...
... see Ref [25]) The genetic selection and breeding activities of a few commercial conifer species are being expanded to include genetic mapping and marker development Candidate gene approaches are ... clues to the role of PgMYB11 in conifers The expression profiles of a few of the spruce MYB genes are consistent with previous reports describing the putative function of homologous genes For example, ... Phylogenetic relationships andgene family structure of conifer R2R3-MYBs We used the Mega 2.0 method to construct a phylogenetic tree using full length cDNA sequences (Fig 2) The result of our...
... in gene expression: meaning and relevance to the cell cycle It is of interest to look at the basic data in Table of Liu, Gaido and Wolfinger [1] In Table they present results on the geneexpression ... recombined together and analyzed These combined cells should not give a variable pattern ofgeneexpression This controls for perturbation of the culture by the selection method 15) Results using different ... different experiments in terms of variation in the phase angles of peaking ofgeneexpression in different individual cells Thus, given a particular variation in geneexpression in different cells,...
... of production relative to its rate of degradation The quantity of a protein produced in the cell is affected by the expression level of its mRNA transcript The levels ofgeneexpression are controlled ... between transcript expression levels and the amount of disorder are not dictated by function class bias and represent genuine and robust features of the data Absolute geneexpression profiles for highly ... the down-regulation ofgeneexpression The absolute geneexpression levels and the predicted miRNA regulation are anti-correlated The overall decrease in the geneexpressionof the transcripts...
... Effect of hypoxia and over -expression of hChK on invasion potential of PC-3 and LNCaP prostatecancer cells 87 Figure 31 Effect of hypoxia and over -expression of hChK on expressionof promigratory ... Effect of over -expression of hChK on cell morphology ofprostatecancer cells 84 Figure 29 Effect of over -expression of hChK on anchorage independent growth of LNCaP prostatecancer ... over -expression of choline kinase and hypoxia on invasion potential ofprostatecancer cells 86 4.6 Effect of over -expression of choline kinase and hypoxia on expressionof pro-invasion...