coimmobilization of cmcase and b glucosidase on yeast cell surface

báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx

... The ubiquitination of crude recombinant UPL7 and UPL5 was detected by luminescent analysis described in B Bio-Ub means biotinylated ubiquitin D, polyubiquitination of crude recombinant UPL7 and ... loaded, and detected by immunoblot analysis using antiFLAG antibody B, Schematic diagram of detection of ubiquitin-conjugation of UPLs by luminescent analysis Protein Aconjugated acceptor beads and ... Detection of E3-independent polyubiquitination of AtUBC22 by luminescent analysis A, Polyubiquitin chain on AtUBC22 but not on AtUBC35 was detected by immunoblot analysis In this assay, polyubiquitination...

Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx

Danh mục: Báo cáo khoa học

... expression and function is highly variable Roles have been described in the secretion, stability and oligomerization of proteins [38,39], the bioactivities of enzymes [40] and the binding afﬁnities of ... is capable of b2 M interaction and cell- surface expression but is unable to regulate cellular iron uptake like the wild-type HFE protein [4,15] Although much insight into HFE function has been gained ... transduction J Biol Chem 264, 2409–2414 Makanji Y, Harrison CA, Stanton PG, Krishna R & Robertson DM (2007) Inhibin A and B in vitro bioactivities are modiﬁed by their degree of glycosylation and their...

Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen afﬁnities of the a and b subunits potx

Danh mục: Báo cáo khoa học

... association rate constant of O2 rebinding and the 6110 quantum yield of BR for the a and b subunits within the liganded dimer and tetramer of hemoglobin were determined Second, pH and NaCl effects on ... Correlations between the values of the association rate constant of BR (k¢) and the quantum yield of BR (c) The data for the a and b subunits within liganded tetrameric HbA are shown in (A) and (B) , ... 153–311 47 Bucci E & Fronticelli C (1965) A new method for the preparation of a and b subunits of human hemoglobin J Biol Chem 240, 551–552 pH and NaCl effects on HbA subunits oxygenation 48 Haire...

Báo cáo y học: "Could the expression of CD86 and FcγRIIB on B cells be functionally related and involved in driving rheumatoid arthritis" pps

Danh mục: Báo cáo khoa học

... inhibitory FcγRIIB and the modulation of B cell activity and humoral tolerance has also been translated into humans Expression of FcγRIIB was found to be considerably decreased in memory B cells from ... pathogenesis of RA has not been demonstrated FcγRIIB is diﬀerentially expressed on healthy B cells, depending on the stage of B cell development and, since FcγRIIB helps to regulate B cell receptor-mediated ... expression and levels of FcγRIIB, indicating that the relationship between FcγRIIB expression and B cell activation, especially in the context of inﬂammatory disease, requires further exploration...

Báo cáo y học: "Comparison of Classical and Clozapine Treatment on Schizophrenia Using Positive and Negative Syndrome Scale of Schizophrenia (PANSS) and SPECT Imaging"

Danh mục: Y học thưởng thức

... include birth in cities, birth in winter and early spring, and viral infections in the mother during the second and third trimesters of gestation A strong association between hypofrontality and negative ... rest: normal distribution and effect of age and gender Eur J Nucl Med 2002; 29: 67-75 Erbas B, Kumbasar H, Erbengi G, Bekdik C Tc-99m HMPAO/SPECT determination of regional cerebral blood flow changes ... correlation between negative symptoms and hypofrontality in both groups, especially in the left superior frontal (Table 5) This relationship between negative symptoms and frontal hypo-perfusion has been...

EFFECTS OF SPILLED AND STRANDED OILS ON SEAWATER INFILTRATION AND MACROBENTHIC COMMUNITY IN TIDAL FLATS

Danh mục: Môi trường

... water content of the sediments between high and low tides according to the method described by Cheong (2000) - 24 - Journal of Water and Environment Technology, Vol.2, No.1, 2004 Concentration of ... non-degradable fraction in oil and usually being used as a biomarker Fig.7 shows vertical profiles of the oil concentrations in the sediments in the O simulator The oil concentration in the surface ... Infiltration rate of seawater by tide at day 23 and 58 in the C and O simulators Fig.8 shows infiltration rate of seawater by tidal action both in the C and O simulators The infiltration rates of sweater...

Effect of Calcium and Magnesium Addition on Arsenic Leaching from Paddy Field Soil of Bangladesh

Danh mục: Môi trường

... from highly contaminated paddy field soil under different concentrations of Ca and Mg addition, pH conditions and anaerobic incubation The influence of phosphorus (P) on As release with and without ... decrease of Fe leaching Poor correlation was obtained between the concentrations of Fe and As leached upon addition of Ca (R2 = 0.05) and Mg (R2 = 0.15) Fig shows the adsorbed amount of Ca and Mg ... corresponding decrease in the soluble As concentration with the increase of Ca and Mg addition Strong correlation was obtained between leachate Fe and As for Ca addition (R2 = 0.96; p < 0.01) and...

Optimization of design and operating parameters on the year round performance of a multi-stage evacuated solar desalination system using transient mathematical analysis

Danh mục: Vật lý

... also because of the absence of non-condensable gases heat transfer by radiation and natural convection are limited Thus, heat transfer between the hot saline water bed and the condensation surface ... variation of global solar radiation and the ambient temperature for the months of July to December The maximum radiation occurs in the month of March of 955.56 W/m2 at 13:00 and minimum in December ... has been performed only for the month of March 5.6 Effect of series and parallel combination of flat plate collectors The combination of flat plate collectors in both series and parallel are considered...

Tài liệu The Transformation of Banking and Its Impact on Consumers and Small Businesses docx

Danh mục: Ngân hàng - Tín dụng

... Source: Summary of Deposits, National Information Center Database II IMPACT OF THE CHANGES ON CONSUMERS Consumers have traditionally relied on nearby banks and branches for many of their banking services ... Reports of Condition and Income, National Information Center Database deposit share has come at the expense of regional and super-regional banking organizations, those in the $10–100 billion range ... Chart DEPOSIT DISTRIBUTION BY SIZE OF BANKING ORGANIZATION Percent of total deposits 50 1989 40 1994 1999 30 20 10 $10 0b Size of organization (1999 dollars)...

Tài liệu Freedom of Expression on the Internet - A study of legal provisions and practices related to freedom of expression, the free flow of information and media pluralism on the Internet in OSCE participating States ppt

Danh mục: Quản trị mạng

... characteristics, and terms and conditions of their broadband services; ii No blocking Fixed broadband providers may not block lawful content, applications, services, or non-harmful devices; mobile broadband ... Meeting of the Conference on the Human Dimension of the CSCE and in breach of Article 19 of the International Covenant on Civil and Political Rights and Article 10 of the European Convention on Human ... of appreciation afforded to them by international norms and standards Blocking of online content can only be justified if in accordance with these standards and done pursuant to court order and...

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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

Danh mục: Báo cáo khoa học

... LlCBP33A has a preference for A B C Binding preferences for LlCBP33A Some family 33 CBPs bind to a broad selection of insoluble carbohydrates (e.g ChbB, which binds both a- and b- chitin [21], and ... A B Fig Structural comparison of CBP21 and LlCBP33A Illustrations of the CBP21 structure (A) and a structural model of LlCBP33A (B) shown in a surface representation The surface thought to be ... concentrations Sequence analysis and modelling of LlChi18A and LlCBP33A Cloning and purication of LlChi18A and LlCBP33A The gene fragments coding for the mature proteins of LlChi18A and LlCBP33A...

Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc

Danh mục: Báo cáo khoa học

... be used to derive kinetic and equilibrium constants and also allows Ó FEBS 2002 Assembly of RuvA and RuvC on Holliday junctions (Eur J Biochem 269) 5495 the calculation of the stoichiometry of ... pin of E coli RuvA modulates Holliday junction resolution by preventing binding to duplex DNA and constraining the role of branch migration in the RuvAB complex [21] One of the enduring questions ... concentration, equilibrium is soon reached with only small amounts of additional binding to the junction at concentrations above this value Therefore over only a 10-fold increase in protein concentration,...

Business water footprint accounting: A tool to assess how production of goods and services impacts on freshwater resources worldwide pdf

Danh mục: Kế toán - Kiểm toán

... terms of shareholder value but also in terms of the long-term continuity value of business to communities In the ongoing debate on globalization, concerns have been expressed about the sustainability ... questions The efficient use of freshwater and control of pollution is often part of sustainability issues addressed by business In the last ten years, initiatives were the foundation of the World Business ... impacts of business on society, especially of multinational corporations (OECD, 2001) The sustainability concept is determined by three components: a social, economic, and environmental one (WCED,...

Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

Danh mục: Báo cáo khoa học

... hydrogen bondages (B) Blue balls represent nitrogen, red balls oxygen, and yellow balls carbon of amino acids Amino acid residues in one subunit are primed and those in the other subunit are ... dissociation/ association of CDase I-5 Based on the information obtained about the 3D structure of CDase I-5, the quaternary state of CDase I-5 was likely to be maintained by the intrinsic capability of ... Dodecamerization would expand the opportunities for the regulation of an enzyme by providing a number of functional advantages, such as high stability and control over the accessibility and specicity of...

EFFECTS OF TARIFFS AND SANITARY BARRIERS ON HIGH- AND LOW-VALUE POULTRY TRADE potx

Danh mục: Nông nghiệp

... elasticity value of 0.5 for all regions Based on the assumed values of the elasticities of substitution and the initial consumption budget shares, all poultry products are gross substitutes for one another ... Globalization and the Smallholders: A Review of Issues, Approaches, and Implications, November 2002 by Sudha Narayanan and Ashok Gulati 51 Rice Trade Liberalization and Poverty, November 2002 by ... Availability and Demand in 2020, October 1997 by Christopher Delgado, Pierre Crosson, and Claude Courbois 20 Rural Economy and Farm Income Diversification in Developing Countries, October 1997 by...

Effect of soaking and gaseous treatment on GABA content ingerminatedbrownrice

Danh mục: Sinh học

... Comparison of GABA contents in GBR between soaking and SGT methods Table Effect of GBR by steaming and ethanol treatment on GABA content and APC Treatment GABA (mg/100 g FW) APC (cfu gÀ1) Control (brown ... measured the effect of steaming and ethanol treatment on the GABA content of GBR Table shows that the GABA content in GBR–SGT was 11.3 mg, and that GABA content was not affected by steaming and ethanol ... reduced the viable bacterial count in brown rice, but number of bacterial spores subsequently increased (Kinefuchi et al., 1999a, 199 9b) Consequently, only the combination of steaming and ethanol...

Effects of compost and phosphate amendments on arsenic mobility in soils and arsenic uptake by the hyper

Danh mục: Môi trường

... 0) Control 5.76bb c MSW 7.40a BS 6.33ab PR 6.22ab 16.2ab 18.2a 18.8a 19.1a 34.1a 33.7a 31. 0b 31. 0b 80.2a 71.4bc 75. 0b 78.1ab 5.28a 6.20a 5.28a 5.32a 31.2a 27.0bc 31.2a 28. 6b 56. 4b 59.4a 56. 8b 57.8ab ... carbon in the biosolids Arsenic concentrations in both composts and PR were negligible, compared with that in the CCA and ASC soils (Table 1) 3.2 Eﬀects of soil amendments on soil pH, DOC, and ... up to 2.3% As of dry plant weight from contaminated soils Phytoremediation is feasible since 90% of the arsenic absobed was in the above-ground biomass, and could be removed by frond harvest (Tu...

Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot

Danh mục: Báo cáo khoa học

... Analysis of a b- glucosidase aglycone-binding site A B C D Fig Quaternary structure of wild-type and mutant Zm-p60.1 b- glucosidases (A) Elution profiles of wild-type and mutant Zm-p60.1 b- glucosidases ... orientation of the aglycone moiety remains the same for both proteins because of the strong stacking interaction with W373 Discussion We identified a group of b- glucosidases in members of the Brassicaceae ... Expression of soluble and catalytically active plant (monocot) beta-glucosidases in E coli Biotechnol Bioeng 63, 392–400 12 Babcock GD & Esen A (1994) Substrate specificity of maize beta-glucosidase...

Báo cáo khoa học: Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni doc

Danh mục: Báo cáo khoa học

... acquisition of MS ⁄ MS data In order to obtain extensive MS ⁄ MS data of both sodium and proton adducts, each peak fraction was analysed by nano-LC-MS both with and without addition of sodium ... Normal-phase HPLC separation of 2-aminobenzamide-labelled N-glycans from adult S mansoni Peaks are labelled with fraction numbers and glycan compositions of the major 2-aminobenzamide-labelled species detected ... identiﬁed based on retention times and electron-impact fragmentation patterns The elution position of terminal GlcNAc as determined with an authentic standard is indicated by an arrow The 2AB-labelled,...

Báo cáo khoa học: "The effect of domain and text type on text prediction quality" pptx

Danh mục: Báo cáo khoa học

... training on 358 questions and evaluating on the remaining questions) There is some variation among methods in the size and type of buffer used Most methods use character n-grams as buffer, because ... Section we present our approach to text prediction Sections and describe the experiments that we carried out and the results we obtained We phrase our conclusions in Section Possible evaluation ... In both the buffer and the prediction label, any capitalization is kept At each point in the typing process, our algorithm gives one suggestion: the word that is the most likely continuation of...

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