carotovora elicitors and b cinerea trigger cell death in physcomitrella

báo cáo khoa học: " Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patens" pdf

Ngày tải lên : 12/08/2014, 05:20

... suggesting a cell death process E.c carotovora elicitors and B cinerea trigger cell death in Physcomitrella To assess whether CF and B cinerea caused cell death of Physcomitrella tissues, we stained ... ET: Erwinia carotovora subsp carotovora and Erwinia -derived elicitors HrpN and PehA trigger distinct but interacting defense responses and cell death in Arabidopsis Mol Plant-Microbe Interact ... Analysis cell death in Physcomitrella Analysis of cell death in Physcomitrella Evans blue staining of protonemal tissues after treatments with LB (A), CF(SCC3193) (B) , CF(SCC1) (C), PDB (D) and B cinerea...

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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Ngày tải lên : 18/02/2014, 04:20

... of GRASP55 and MT1-MMP in bbHT1080 Fixed and permeabilized bbHT1080 cells were incubated with antibodies directed against MT1-MMP (A, D, J), GRASP55 (G, K), TGN46 (E) and giantin (B, H) The co-localization ... subsequent to its fusion to the GAL4 DNA binding domain We therefore cannot rule out an interaction between MT1-MMP ICD and the GRASP55 PDZ1 domain GRASP55 binds to furin, PC5/ 6B and PC7 intracellular ... Margolis B & Birnbaum D (2000) ERBIN: a basolateral PDZ protein that interacts with the mammalian ERBB2/HER2 receptor Nat Cell Biol 2, 407–414 47 Cohen NA, Brenman JE, Snyder SH & Bredt DS (1996) Binding...

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Báo cáo khoa học: Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b doc

Ngày tải lên : 29/03/2014, 08:20

... vector (C) were incubated with p15–proSN wild type probe (all lanes in A and C, lanes 1–4 in B and lane 1–2 in D) or p15–proSN* mutant probe (lanes 5–8 in B and lanes 3–4 in D) followed by EMSA For ... higher Interestingly, simultaneous binding of EGR-1 and SP-1 to adjacent DNA-binding sites was also shown to be required for maximal activity of the interleukin-2 receptor b- chain promoter suggesting ... untreated HepG2 Both bands further increased by 6.0- to 8.0-fold at h and decreased at h Another band, SNIII, had a rather abundant basal level, increased by 2.5- and 5.0-fold at and h, respectively,...

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Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx

Ngày tải lên : 31/03/2014, 01:20

... calcium-activation of gelsolin prior to binding actin Gelsolin G2 and G4–6 domains were labeled by FITC as described elsewhere [29] Biotinylation of G2 domain by BACNHS was performed as reported ... V170-V657, with G6 In summary, G2 binds to G4–6 through two distinct interfaces Binding site involves G2 203–225 and G6 744–755 and binding site involves G2 region around R168-R69 and G6 D669-D670 ... critical for actin binding EMBO J 9, 4103–4109 24 Way, M., Pope, B & Weeds, A.G (1992) Evidence for functional homology in the F-actin binding domains of gelsolin and alphaactinin: implications...

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báo cáo khoa học: "Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity" pps

Ngày tải lên : 11/08/2014, 00:23

... was eliminated at lower temperature (4°C) in THP-1 and A549 cells [60] Sodium azide is widely used both in vivo and in vitro as an inhibitor of cellular respiration It acts by inhibiting cytochrome ... staining intensity changes for all tested doses in the THP-1 cell line, (Figure 4C, 4E) The lysosomal mass/ pH staining intensity was increased in A549 cells following 24 h exposure to 20 and ... cell viability, cell membrane permeability and lysosomal pH which are toxicity-attributed phenomena Variations in cell membrane permeability, measured as changes in luminescence intensity, indicated...

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báo cáo khoa học: " WRKY Transcription Factors Involved in Activation of SA Biosynthesis Genes" doc

Ngày tải lên : 11/08/2014, 11:20

... core, in one of the binding sites an A is present at +1, which in the W-box is usually either a C or a T To disable binding of WRKY28 to the 30-bp EMSA probe harboring the binding sites at -460 and ... containing the two binding sites (Results not shown) In conclusion, the ChIP assays indicated that WRKY28 specifically binds to the ICS1 promoter in vivo, most probably to one or both binding ... Minus signs above the lanes indicate binding mixtures without recombinant protein The position of the protein-DNA complexes is indicated by an arrow Plus and minus signs in panel B indicate the...

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báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot

Ngày tải lên : 11/08/2014, 11:21

... metallothionein-like gene, the universal stress protein, profilin &mlo Metallothioneins inhibit programmed cell death (PCD) and Fumonisin B1 -induced root death in tomato infected with Agrobacterium ... an increase in ROS and NO levels and deduced that the increase in ROS observed may assist in weakening host tissue early in infection with the sharp increase in NO possibly resulting in salicylic ... three libraries for pyrosequencing and designated as uninfected, library and library The hr time point and was regarded as the uninfected library with library containing the & 12 hr infection...

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báo cáo khoa học: " Constitutive Expressor of Pathogenesis-Related Genes5 affects cell wall biogenesis and trichome development" potx

Ngày tải lên : 12/08/2014, 05:20

... Myb DNA-binding domain, but lacks a transcription activation domain, and can bind to GL3 in yeast, suggesting that it directly inhibits function of the GL1/GL3/TTG complex in cells neighboring ... development, and is thought to prevent neighboring cells from developing as trichomes by diffusing to neighboring cells via plasmodesmata and inhibiting trichome development in a classic lateral inhibition ... Kirik V, Bouyer D, Schobinger U, Bechtold N, Herzog M, Bonneville JM, Hulskamp M: CPR5 is involved in cell proliferation and cell death control and encodes a novel transmembrane protein Curr Biol...

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A study of new and advanced control charts for two categories of time related processes

Ngày tải lên : 10/09/2015, 09:11

... theory and distribution models have also been widely used in structural engineering, material strength and reliability analysis Beretta and Murakami (1998) investigated the required minimum number ... chart and variable TBE control chart Instead of monitoring the quantity or the proportion of events occurring during certain sampling interval, TBE charts devote the attention to the time between ... detecting shift in directions of (d, d) and outperforms the traditional MEWMA chart for both the raw and transformed GBE data in both states monitoring as well Chapter summarizes all the findings and...

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Transcriptome analysis and expression of immune related genes in megalobrama amblycephala after challenge with aeromonas hydrophila

Ngày tải lên : 21/05/2016, 23:07

... sequence of its transcribed DNA (Brown, 2002) Based on analyzing the entire collection of RNAs in a cell facilitates determining the beginning and ending of a gene in the cells and tissues of an organism ... and experimental inoculated bacterial strain (D4) in sensitive ability to antimicrobials, and also to determine the suitable therapeutical medicines for controlling Aeromonas infection in BSB ... I, IIa, IIb) (Luo et al., 201 4b) of BSB in China However, only few reports that involving in immune-related genes of BSB are certainly available (Ding et al., 2013; Luo et al., 201 4b; Ma et al.,...

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Tài liệu Báo cáo khoa học: Myristoylation of the dual-speciﬁcity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

Ngày tải lên : 16/02/2014, 14:20

... apoptosis In the extrinsic pathway, binding of death ligands to their respective receptors recruit adaptor proteins, such as Fas-associated death domain protein, which in turn bind and aggregate FEBS ... signs of apoptotic cell death, including condensed chromatin in the nucleus Further analysis revealed that ﬂoating could be inhibited by treating the cells with a pan-caspase inhibitor, Z-VAD-FMK, ... according to standard protocols [20] using lm labeled substrate and 0.5 or lg recombinant JSP1 Counting of ﬂoating cells Cos-1 cells were treated with 50 lm pan-caspase inhibitor Z-VAD-FMK (BIOMOL,...

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RAN D OM WALK IN RANDOM AND NO N- RAND OM ENVIRONMENTS h E C D N D E D I T I O N pdf

Ngày tải lên : 06/03/2014, 19:20

... Putting So = So(z)= and S, = S,(z) = Cy=l ri(z) ( n = , , ) we obtain (1.3) 1.4 Coin tossing Two gamblers (A and B) are tossing a coin A wins one dollar if the tossing results in a head and B ... The gambler will talk about coin tossing and his gain And a probabilist will consider independent, identically distributed random variables and the partial sums of those Mathematically speaking ... WC2H 9HE Library of Congress Cataloging -in- Publication Data Random walk in random and non-random environments / Pfll RCvCsz. 2nd ed p cm Includes bibliographical references and indexes ISBN 981-256-361-X...

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Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc

Ngày tải lên : 08/03/2014, 08:20

... labeling medium containing the inhibitors as described above and incubated for 17 h FACS analysis and sorting Cells were trypsinized and resuspended in NaCl/Pi containing 10 mM EDTA and 1–2% FBS ... treated with inhibitors was determined from radiolabeled cells Cells were grown in 24 well plates and preincubated with the c-secretase inhibitors in starvation medium After one hour incubation the ... other inhibitors were also applied in the COS-7 cell ﬂuorescence assay, including in particular proteasome and caspase inhibitors The inhibitors lactacystin (0.5 lM and lM), ALLN (10 lM) and MG132...

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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

Ngày tải lên : 30/03/2014, 10:20

... subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4] The c ⁄ e and d subunits play a major role in shaping the ligand-binding sites and also in maintaining ... only binding of antiFLAG mAb was tested CD spectra Binding of the rabbit anti -b serum to recombinant b1 –221 and human AChR When the b subunit ECD was subjected to far-UV CD analysis to examine ... features Binding of mAbs to the ECDs using ELISA Binding of human MG antibodies to recombinant ECDs ELISAs were performed using the conformationdependent mAbs 73 (binds to an epitope on the extracellular...

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Báo cáo khoa học: PKA independent and cell type speciﬁc activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx

Ngày tải lên : 30/03/2014, 16:20

... Cdx-2 allele be insufﬁcient in maintaining its own expression in the intestinal nonendocrine cells, and in preventing the formation of intestinal polyps for the nonendocrine intestinal epithelia? ... 10–25], including the genes that encode proglucagon and insulin in pancreatic islets and intestinal endocrine cells [4–6] Within the intestinal polyps in the Cdx-2+ ⁄ – mice, the remaining wild ... Cdx-2 protein expression by forskolin ⁄ IBMX is caused by increasing intracellular levels of cAMP, the cell membrane permeable cAMP analogue, 8-Br-cAMP, was utilized for the InR1-G9 cell line (Fig...

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Project Gutenberg''''s Researches on Cellulose, by C. F. Cross and E. J. Bevan pptx

Ngày tải lên : 28/06/2014, 19:20

... measure the probability of finding a working career in connection with the cellulose industries They will not find this invitation in the treatment accorded to the subject in text-books and lectures ... systematic, and, in fact, inevitable Cellulose is in the first instance a structure, and the anatomical relationships supply a certain basis of classification Next, it is known to us and is defined by ... subject-matter Thus 'the carbohydrates' may be said to have been in the inchoate condition, qualified by a certain classification, prior to the pioneering investigations of Fischer In attacking...

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Báo cáo toán học: " Hadamard matrices and strongly regular graphs with the 3-e.c. adjacency property" pdf

Ngày tải lên : 07/08/2014, 06:22

... J Combin Theory 11 (1971), 38–44 [8] L Caccetta, L., P Erd˝s, and K Vijayan, A property of random graphs, Ars Combin o 19 (1985), 287–294 [9] R Fagin, Probabilities on ﬁnite models, J Symbolic ... following condition: for each distinct rows r1 , r2 , r3 from A, representing vertices x, y, z in G, there are columns in the submatrix formed by r1 , r2 , r3 , distinct from the columns representing ... the linear system a +b+ c+d+e+f +g+h a +b+ c+d a +b+ e+f a+c+e+g a +b c−d−e−f +g+h a b+ c−d−e+f −g+h a b c+d+e−f −g+h = = = = = = = 4n 2n 2n 2n 0 It can be seen that the solution for this system is b =...

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Báo cáo y học: "α Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism" pps

Ngày tải lên : 09/08/2014, 01:23

... DΙDonato JA, Williams κ BRG: NF- B activation by double-stranded-RNA-activated κ protein kinase (PKR) is mediated through NF- B- inducing κ kinase and I B kinase Mol Cell Biol 2000, 20:1278-1290 ... buffer (0.12 M Tris/HCl (pH 6.8), containing 4% (w/v) SDS, 20% (v/v) glycerol, and 0.01% (w/v) bromophenol blue), a recombinant MMP-9 standard (1 µl) (Oncogene, Nottingham, UK), bovine-fibroblast-conditioned ... the interferon-inducible kinase PKR J Biol Chem 1995, 270:3100-3106 Ito T, Jagus R, May WS: Interleukin stimulates protein synthesis by regulating double-stranded RNA-dependant protein kinase...

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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc

Ngày tải lên : 11/08/2014, 08:20

... anti-Neuropilin (sc-5541) antibodies (2 µg/ml, Santa Cruz Biotechnology) in blocking buffer at 4°C o/n Unbound primary antibodies were removed by washing, and the cells were incubated in 20 mM ammonium ... Results in BioMedicine 2006, 5:8 http://www.jnrbm.com/content/5/1/8 lines, PC12 and CPAE cells were incubated with AP fusion proteins The AP-mVEGF164 affinity probe strongly stained proliferating and ... endostatin affinity probes derived from collagens XVIII and XV not bind to PC12 cells indicating that these cells not express endostatin cell membrane receptors This observation is consistent with absent...

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Activation of c h and c f bonds by cyclopentadienyl iridium complexes

Ngày tải lên : 13/09/2015, 19:48

... mentorship, inspiration, invaluable advice and help; my co-supervisors, A/P Marc Garland and Dr Zhu Yinghuai (Institute of Chemical and Engineering Sciences, A*STAR) for their support and my boss, Andy ... Gao Lu, Mui Ling, Meien and Jialin for maintaining a comfortable working environment in the lab I also wish to thank Karl I Krummel (Department of Chemical and Biomolecular Engineering, NUS) for ... different types of C-H bonds by various metal fragments on per hydrogen basis 11 2.1 Changes in 3a:2a absorbance ratio with length of photolysis 35 2.2 Changes in 3b: 2b and 4b: 2b absorbance ratio with...

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