carotovora cfs and b cinerea cause disease symptoms in physcomitrella

báo cáo khoa học: " Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patens" pdf

... cells E.c carotovora, CFs and B cinerea cause disease symptoms in Physcomitrella Development of disease symptoms by E.c carotovora was initiated by inoculating the harpin HrpN-producing E.c carotovoraSCC1 ... treatment and B cinerea inoculation cause disease symptoms and induce defense responses in Physcomitrella CF(SCC1), CF(SCC3193) and B cinerea induced the expression of defense-related genes, including ... CF(SCC3193) and B cinerea are capable of causing disease symptoms in Physcomitrella CF(SCC1) and B cinerea trigger cytoplasmic shrinkage, accumulation of autofluorescent compounds and chloroplast browning...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

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... AMPA binding protein and glutamate receptor interacting protein (GRIP) It would therefore be interesting to determine whether this motif is also involved in the interaction between MT5-MMP and ... PDZ1 subsequent to its fusion to the GAL4 DNA binding domain We therefore cannot rule out an interaction between MT1-MMP ICD and the GRASP55 PDZ1 domain GRASP55 binds to furin, PC5/ 6B and PC7 intracellular ... of GRASP55 and MT1-MMP in bbHT1080 Fixed and permeabilized bbHT1080 cells were incubated with antibodies directed against MT1-MMP (A, D, J), GRASP55 (G, K), TGN46 (E) and giantin (B, H) The co-localization...

Báo cáo khoa học: Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b doc

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... vector (C) were incubated with p15–proSN wild type probe (all lanes in A and C, lanes 1–4 in B and lane 1–2 in D) or p15–proSN* mutant probe (lanes 5–8 in B and lanes 3–4 in D) followed by EMSA For ... higher Interestingly, simultaneous binding of EGR-1 and SP-1 to adjacent DNA-binding sites was also shown to be required for maximal activity of the interleukin-2 receptor b- chain promoter suggesting ... could be contained within the band shifts As shown in Fig 9C, SNII and SNIII were suppressed by 95 and 65% at h if the EMSA reaction mix was preincubated with Snail antibody, but not Raf antibody...

Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx

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... calcium-activation of gelsolin prior to binding actin Gelsolin G2 and G4–6 domains were labeled by FITC as described elsewhere [29] Biotinylation of G2 domain by BACNHS was performed as reported ... V170-V657, with G6 In summary, G2 binds to G4–6 through two distinct interfaces Binding site involves G2 203–225 and G6 744–755 and binding site involves G2 region around R168-R69 and G6 D669-D670 ... critical for actin binding EMBO J 9, 4103–4109 24 Way, M., Pope, B & Weeds, A.G (1992) Evidence for functional homology in the F-actin binding domains of gelsolin and alphaactinin: implications...

báo cáo khoa học: "Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity" pps

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... technology is becoming an indispensable approach to research and industry, assisting in understanding complex cellular processes in disease pathogenesis [36], drug target validation and drug lead ... Scientific Inc., USA) Briefly, cells seeded in 96-wells plates as described above were incubated with the above mentioned SiO2NP, for different intervals as previously indicated in the text, and in addition ... blotting for h The PVDF membranes were blocked in 5% non-fat dry milk in PBS Tween20 (PBST) [0.1% (v/v) Tween20 in phosphate buffered saline (PBS)] for h at room temperature After washing, the blots...

báo cáo khoa học: " WRKY Transcription Factors Involved in Activation of SA Biosynthesis Genes" doc

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... core, in one of the binding sites an A is present at +1, which in the W-box is usually either a C or a T To disable binding of WRKY28 to the 30-bp EMSA probe harboring the binding sites at -460 and ... containing the two binding sites (Results not shown) In conclusion, the ChIP assays indicated that WRKY28 specifically binds to the ICS1 promoter in vivo, most probably to one or both binding ... Minus signs above the lanes indicate binding mixtures without recombinant protein The position of the protein-DNA complexes is indicated by an arrow Plus and minus signs in panel B indicate the...

báo cáo khoa học: "EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi" pot

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... an increase in ROS and NO levels and deduced that the increase in ROS observed may assist in weakening host tissue early in infection with the sharp increase in NO possibly resulting in salicylic ... three libraries for pyrosequencing and designated as uninfected, library and library The hr time point and was regarded as the uninfected library with library containing the & 12 hr infection ... metallothionein-like gene, the universal stress protein, profilin &mlo Metallothioneins inhibit programmed cell death (PCD) and Fumonisin B1 -induced root death in tomato infected with Agrobacterium...

báo cáo khoa học: " Constitutive Expressor of Pathogenesis-Related Genes5 affects cell wall biogenesis and trichome development" potx

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... Myb DNA-binding domain, but lacks a transcription activation domain, and can bind to GL3 in yeast, suggesting that it directly inhibits function of the GL1/GL3/TTG complex in cells neighboring ... development, and is thought to prevent neighboring cells from developing as trichomes by diffusing to neighboring cells via plasmodesmata and inhibiting trichome development in a classic lateral inhibition ... Weinl C, Bouyer D, Schobinger U, Hulskamp M: Misexpression of the cyclin-dependent kinase inhibitor ICK1/KRP1 in single-celled Arabidopsis trichomes reduces endoreduplication and cell size and...

A study of new and advanced control charts for two categories of time related processes

Danh mục: Cao đẳng - Đại học

... theory and distribution models have also been widely used in structural engineering, material strength and reliability analysis Beretta and Murakami (1998) investigated the required minimum number ... chart and variable TBE control chart Instead of monitoring the quantity or the proportion of events occurring during certain sampling interval, TBE charts devote the attention to the time between ... detecting shift in directions of (d, d) and outperforms the traditional MEWMA chart for both the raw and transformed GBE data in both states monitoring as well Chapter summarizes all the findings and...

Transcriptome analysis and expression of immune related genes in megalobrama amblycephala after challenge with aeromonas hydrophila

Danh mục: Tổng hợp

... and experimental inoculated bacterial strain (D4) in sensitive ability to antimicrobials, and also to determine the suitable therapeutical medicines for controlling Aeromonas infection in BSB ... I, IIa, IIb) (Luo et al., 201 4b) of BSB in China However, only few reports that involving in immune-related genes of BSB are certainly available (Ding et al., 2013; Luo et al., 201 4b; Ma et al., ... reported to be sensitive to chloramphenicol, erythromycin, kanamycin, neomycin (Boonyaratpalin, 1989) and resistant to amoxicillin and clindamycin (Adanir and Turutoglu, 2007; Belém-Costa and Cyrino,...

Tài liệu Báo cáo khoa học: Myristoylation of the dual-speciﬁcity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

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... mitogenactivated protein kinase signal transduction pathways activated by stress and in ammation Physiol Rev 81, 807–869 Pearson G, Robinson F, Beers GibsonT, Xu BE, Karandikar M, Berman K & Cobb MH (2001) ... according to standard protocols [20] using lm labeled substrate and 0.5 or lg recombinant JSP1 Counting of ﬂoating cells Cos-1 cells were treated with 50 lm pan-caspase inhibitor Z-VAD-FMK (BIOMOL, ... apoptosis initially become rounded, which is accompanied or followed by membrane blebbing, resulting in small vesicles termed apoptotic bodies Inside the cell, apoptosis is characterized by condensation...

RAN D OM WALK IN RANDOM AND NO N- RAND OM ENVIRONMENTS h E C D N D E D I T I O N pdf

Danh mục: Điện - Điện tử

... Putting So = So(z)= and S, = S,(z) = Cy=l ri(z) ( n = , , ) we obtain (1.3) 1.4 Coin tossing Two gamblers (A and B) are tossing a coin A wins one dollar if the tossing results in a head and B ... The gambler will talk about coin tossing and his gain And a probabilist will consider independent, identically distributed random variables and the partial sums of those Mathematically speaking ... WC2H 9HE Library of Congress Cataloging -in- Publication Data Random walk in random and non-random environments / Pfll RCvCsz. 2nd ed p cm Includes bibliographical references and indexes ISBN 981-256-361-X...

Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc

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... labeling medium containing the inhibitors as described above and incubated for 17 h FACS analysis and sorting Cells were trypsinized and resuspended in NaCl/Pi containing 10 mM EDTA and 1–2% FBS ... other inhibitors were also applied in the COS-7 cell ﬂuorescence assay, including in particular proteasome and caspase inhibitors The inhibitors lactacystin (0.5 lM and lM), ALLN (10 lM) and MG132 ... electrophoresis and Western blotting To analyze protein expression and processing, cells were starved for 45 in methionine- and cysteine-free medium (ICN), pulsed for 30 in medium containing mCiÆmL)1...

Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

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... subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4] The c ⁄ e and d subunits play a major role in shaping the ligand-binding sites and also in maintaining ... only binding of antiFLAG mAb was tested CD spectra Binding of the rabbit anti -b serum to recombinant b1 –221 and human AChR When the b subunit ECD was subjected to far-UV CD analysis to examine ... Lymnaea stagnalis [11] and Bullinus truncatus [29] acetylcholine-binding proteins, which provide the prototypes for the AChR ligand-binding domain, show a predominance of b- sheet, and the CD spectra...

Báo cáo khoa học: PKA independent and cell type speciﬁc activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx

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... Cdx-2 allele be insufficient in maintaining its own expression in the intestinal nonendocrine cells, and in preventing the formation of intestinal polyps for the nonendocrine intestinal epithelia? ... 10–25], including the genes that encode proglucagon and insulin in pancreatic islets and intestinal endocrine cells [4–6] Within the intestinal polyps in the Cdx-2+ ⁄ – mice, the remaining wild ... Thomasset M, Traber PG & Perret C (1996) Cis-Acting elements and transcription factors involved in the intestinal specific expression of the rat calbindin-D9K gene: binding of the intestine-specific...

Project Gutenberg''''s Researches on Cellulose, by C. F. Cross and E. J. Bevan pptx

Danh mục: Hóa học - Dầu khí

... the cellulose industries They will not find this invitation in the treatment accorded to the subject in text-books and lectures It is probable, indeed, that the impression produced by their studies ... subject-matter Thus 'the carbohydrates' may be said to have been in the inchoate condition, qualified by a certain classification, prior to the pioneering investigations of Fischer In attacking ... systematic, and, in fact, inevitable Cellulose is in the first instance a structure, and the anatomical relationships supply a certain basis of classification Next, it is known to us and is defined by...

Báo cáo toán học: " Hadamard matrices and strongly regular graphs with the 3-e.c. adjacency property" pdf

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... J Combin Theory 11 (1971), 38–44 [8] L Caccetta, L., P Erd˝s, and K Vijayan, A property of random graphs, Ars Combin o 19 (1985), 287–294 [9] R Fagin, Probabilities on ﬁnite models, J Symbolic ... following condition: for each distinct rows r1 , r2 , r3 from A, representing vertices x, y, z in G, there are columns in the submatrix formed by r1 , r2 , r3 , distinct from the columns representing ... the linear system a +b+ c+d+e+f +g+h a +b+ c+d a +b+ e+f a+c+e+g a +b c−d−e−f +g+h a b+ c−d−e+f −g+h a b c+d+e−f −g+h = = = = = = = 4n 2n 2n 2n 0 It can be seen that the solution for this system is b =...

Báo cáo y học: "α Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism" pps

Danh mục: Báo cáo khoa học

... DΙDonato JA, Williams κ BRG: NF- B activation by double-stranded-RNA-activated κ protein kinase (PKR) is mediated through NF- B- inducing κ kinase and I B kinase Mol Cell Biol 2000, 20:1278-1290 ... buffer (0.12 M Tris/HCl (pH 6.8), containing 4% (w/v) SDS, 20% (v/v) glycerol, and 0.01% (w/v) bromophenol blue), a recombinant MMP-9 standard (1 µl) (Oncogene, Nottingham, UK), bovine-fibroblast-conditioned ... the interferon-inducible kinase PKR J Biol Chem 1995, 270:3100-3106 Ito T, Jagus R, May WS: Interleukin stimulates protein synthesis by regulating double-stranded RNA-dependant protein kinase...

Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc

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... subsequently shown to express VEGF receptors 1, and neuropilin-1, only a minor neuroprotective effect was observed for VEGF when compared to NGF saline (TBS) for min, and incubated in blocking ... anti-Neuropilin (sc-5541) antibodies (2 µg/ml, Santa Cruz Biotechnology) in blocking buffer at 4°C o/n Unbound primary antibodies were removed by washing, and the cells were incubated in 20 mM ammonium chloride ... Results in BioMedicine 2006, 5:8 http://www.jnrbm.com/content/5/1/8 lines, PC12 and CPAE cells were incubated with AP fusion proteins The AP-mVEGF164 affinity probe strongly stained proliferating and...

Activation of c h and c f bonds by cyclopentadienyl iridium complexes

Danh mục: Thạc sĩ - Cao học

... mentorship, inspiration, invaluable advice and help; my co-supervisors, A/P Marc Garland and Dr Zhu Yinghuai (Institute of Chemical and Engineering Sciences, A*STAR) for their support and my boss, Andy ... Gao Lu, Mui Ling, Meien and Jialin for maintaining a comfortable working environment in the lab I also wish to thank Karl I Krummel (Department of Chemical and Biomolecular Engineering, NUS) for ... different types of C-H bonds by various metal fragments on per hydrogen basis 11 2.1 Changes in 3a:2a absorbance ratio with length of photolysis 35 2.2 Changes in 3b: 2b and 4b: 2b absorbance ratio with...

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