... atorvastatin- mà chưa đạt tớim c tiêu LDL -C hay CRP- nhiều c ch chữa trị kh c đã đư c chứng minh là giảm đư c LDL -C hay CRP riêng rẽ. Một là thu c ezetimibe, một chất ngăn chận sự hấp thụ cholesterol. ... m c độ chất béo, bằng c ch làm tăng cholesterol tốt (HDL -C) và làm giảm triglyceride c ng giúp giữ m c độ CRP thấp. M C TIÊU CRP Hiện nay c n quá sớm để x c định một phương sách c n c ... nhiều lợi ích lâm sàng khi bệnh nhân c m c độ CRP cao. Ngoài ra statins c n làm giảm m c độ CRP một c ch đ c lập ngoài t c dụng trên cholesterol xấu (LDL -C: low density lipoprotein cholesterol)....
... pháp đo độ đ c (nephelometry). Bình thường CRP huyết thanh < 10 mg/l. CRP ( proteinCreactive ) M C TIÊU: - Hiểu đư c nguồn g c và bản chất c a CRP - Biết đư c ch c năng miễn dịch và ý nghĩa ... sinh. - C y máu (+) là tiêu chuẩn vàng cho chẩn đoán NTH, nhưng trong một số điều kiện th c tế không phải l c nào c ng sẵn c và cho kết quả đáng tin c y. - CRP (C- reactive protein) là một chất ... hữu ích trong vi c theo dõi và c thể góp phần trong vi c giảm thiểu những trường hợp dùng kháng sinh không c n thiết. - Ngoài CRP, cc cytokine và PCT c ng c thể hữu ích trong vi c chẩn đoán...
... (VRT) c p là c p c u Ngoại khoa thường gặp, tỉ lệ chẩn đoán sai c n cao. C- reactiveprotein (CRP) đư c ứng dụng nhiều. Ở Việt Nam, chưa c nghiên c u nào về CRP trong VRT. M c tiêu: X c định ... Keywords: appendicitis, C- reactive protein, laparoscopy. ĐẶT VẤN ĐỀ VRT c p là c p c u Ngoại khoa thường gặp. Tỉ lệ chẩn đoán sai c n cao. CRP đư c ứng dụng nhiều trong chẩn đoán cc bệnh nhiễm ... chưa BC và c BC (p lần lượt là 1,000 và 0,675). C- reactiveprotein máu Giá trị c a CRP trong chẩn đoán VRT c p Bảng 4: Kết quả phân tích hồi quy Logistic chẩn đoán VRT c p theo nồng độ CRP...
... 23-25 C in a 12-hour light/dark cycle with access to standard powdered mice chow and normal water. The scientific project, including animal care was supervised and approved by Animals Ethics Committee ... gene. The product of this nucleotide sequence was a glycine-rich short peptide including 15 amino acids: [N-(Serine-glycine)7- Serine -C] , which was used as a linker to connect the N-terminus ... GenScript Corporation (USA). BCA Protein Assay Kit was purchased from Beyotime Institute of Biotechnology (Jiangshu, China). Construction of recombinant vector PET28a-gAd-GLP-1-A Recombinant...
... c a VTC. Trên lâm sàng nếu c điều kiện nên áp dụng kết hợp cc phương pháp trên để tiên lượng VTC chính x c hơn, hạn chế tử vong trong cc trường hợp VTC nặng c biến chứng. Đối với cc cơ ... tụy. 1.9. CRP, LDH, Hematocrit trong đánh giá m c độ nặng nhẹ c a VTC 1.9.1. CRP trong đánh giá m c độ nặng nhẹ c a VTC - Nguồn g c, bản chất và ch c năng c a CRP. - Ý nghĩa c a sự thay ... thể lan ra tới cc tạng lân c n: lách, đại tràng, khoang sau ph c m c, dạ dày, tá tràng, đi kèm với cc hoại tử c thể chảy máu; chảy máu tại tụy, trong ổ bụng ho c cơ quan kh ccc nang tuyến...
... GSTCD2-1CD2-2CD2-3CD2PD250150Input GSTCD2-1-6-1CD2-1-6-2CD2-1-6-3CD2-1PDIB: -v-KIND250150InputGSTCD2-1-5CD2-1-6CD2-1-7CD2-1-8CD2-1PD250Input GSTCD2-1-1CD2-1-2CD2-1-3CD2-1-4CD2-1PD250150MAP2MBDRIICD1CD2CD31–147148–599600–10991100–15181519–1829767934CD2-1CD2-2CD2-3600CD21099Inter-action600 ... GSTCD2-1-1CD2-1-2CD2-1-3CD2-1-4CD2-1PD250150MAP2MBDRIICD1CD2CD31–147148–599600–10991100–15181519–1829767934CD2-1CD2-2CD2-3600CD21099Inter-action600 767CD2-1Inter-actionCD2-1-1634CD2-1-2667CD2-1-3701CD2-1-4734CD2-1-5735CD2-1-6702CD2-1-7668CD2-1-8635CD2-1-6-1734702CD2-1-6-2744CD2-1-6-3755CDRIIMBDInter-actionABCDEFFig. ... Pharmaceu-tical Co., Ltd, Tokyo, Japan). Transfected cells were visual-ized by EGFP fluorescence and immunocytochemicalstaining with anti-Flag or anti-HA sera. Cell images wereacquired by confocal...
... specificity of action ofan inhibitor for a protein- folding catalyst cannot be determined in vivo.Furthermore, in vitro evidence for the specificity of bacitracin for PDI isscarce, and the mechanism ... speci c inhibitor of PDI activ-ity [6–8]. However, in vitro evidence for the action ofbacitracin as an inhibitor of PDI is scarce, and evi-dence of its specificity for PDI is nonexistent. Bacitracinis ... upper trace shows the PDI-catalyzed reaction, and the lowertrace the PDI-catalyzed reaction in the presence of 1 mM bacitracin.The lines of best fit are to first-order reactions. (B, C) Time courseanalysis...
... response (i.e. occurring when all receptor sites areoccupied) that will be approached by a speci c analyteconcentration specified by the KDof the receptor–ana-lyte interaction. This was clearly not ... theeukaryotic expression vector the pcDNA3, transfected intoHEK 293 cells (CRL-1573 ATCC), high-expressing coloniesselected, and recombinant proteinC purified, as previouslydescribed [45]. ... by chroma-tography on a HiprepÔ 16 ⁄ 10 Q FF column as describedfor proteinC purification. Enzymatically active APC con-centrations were determined by active site titration usingthe APC chromogenic...
... (1990) Mechanism of reductive activation ofcobalamin-dependent methionine synthase: an electronparamagnetic resonance spectroelectrochemical study.Biochemistry 29, 1129–1135.24 Chen Z, Chakraborty ... reducing hMS and returning it to thecatalytic cycle. The turnover of hMS increases as MSRis reduced to the one-electron, two-electron and four-electron reduced states, reflecting a higher concentra-tion ... radioactive assaydescribed above.AcknowledgementsThis study was funded by the UK Biotechnology andBiological Sciences Research Council. N.S. Scrutton isa BBSRC Professorial Research Fellow....
... spongiformencephalopathy in cattle. The infectious agent is a protease resistant iso-form (PrPSc) of a host encoded prion protein (PrP C ). PrPScproteins arecharacterized according to size and glycoform ... cellularprion protein (PrP C ) to a pathological isoform (PrPSc)accompanied by a conformational change from alargely a-helical form into a b-sheet structure [4]. Incontrast to PrP C , the infectious ... Mourton-Gilles C, Negro A, Sorgato MC, Creminon C & GrassiJ (2004) Selective and efficient immunoprecipitation ofthe disease-associated form of the prion protein can bemediated by nonspeci c interactions...
... ester content was quanti-fied by HPLC, as described above. Protein assay Protein concentrations were quantified using the bicinchoni-nic acid assay (Pierce, Rockford, IL, USA) with 60 min ofincubation ... (apoB) protein. Cholesterol and cholesteryl ester accumulationoccurs within 24 h of exposure to glycated low- density lipoprotein, andincreases in a time-dependent manner. Higher cellular cholesteryl ... modification, using iodine mono-chloride [36,60]. Speci c activity (typically 50–100 c. p.m.Æng)1apoB protein) was determined by c- counting(Cobra II; Packard, Downers Grove, IL, USA). Acetylationof...
... structureof the A state of cytochrome c. Biochemistry 39, 12632–12638.18 Sinibaldi F, Howes BD, Smulevich G, Ciaccio C, Coletta M & Santucci R (2003) Anion concentrationmodulates conformation ... Gar-cia LL (1996) Nuclear magnetic resonance studies ofclass I cytochromes c. InCytochrome C. A Multidisci-plinary Approach (Scott RA & Mauk AG, eds), pp.203–284. University Science Books, ... iso-1-cytochrome c gene and replacing itwith the new synthetic horse cytochrome c gene, by usingthe unique NcoI and BamHI sites. The sequence of theexpression construct (pHCyc) was confirmed...
... I C ,was originally isolated as a 23 kDa cytoplasmic protein A B Fig. 3. Fluorescence microscopic analysesof yeast cells producing I C –GFP. (A) Repre-sentative fluorescence images. S. cerevisiaeBY4741icD ... aci-dic conditions below pH 5 [9], support a scenarioinvolving CPY inhibition by I C in the acidic vacuolesof yeast cells.In conclusion, the present study reveals that I C bindsto anionic phospholipid ... with CPY.AB C Fig. 1. Liposome-binding assay for I C and I C –CPY. I C (A) or I C –CPY(B), the final concentration of which was 2 lM, was added to PC-based liposomes (0.5 mgÆmL)1of PE ⁄ PC,...
... Plant Sciences, College of Agricultural & Environmental Sciences, University of California, CA, USAMost proteins found in plastids are encoded in thenuclear genome, translated on cytosolic ribosomes ... this particular regionplay a crucial role in correct sorting of Toc75.AbbreviationsmtHsp70, mitochondrial heat shock protein 70; Plsp1, plastidic type I signal peptidase 1; psToc75, Toc75 from ... aspartic acid, and serine,occur relatively frequently in these regions. Togetherthey account for 17% of residues within this stretch.We replaced the critical tri-glycine within the peaToc75...