biowaivers when in vitro dissolution is independent of dissolution test conditions
Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
... U937 cell apoptosis by maintaining mitochondrial integrity and function [43], and ricin-induced apoptosis of U937 cells by maintaining an intracellular reducing condition by acting as a thiol supplier ... we studied the DISCUSSION In this report, we describe the quantification of changes in mRNA levels of 23 apoptosis-related genes in chorion laeve tissue during in vitro incubation of human fetal ... factor) binds to the Jun domain, and that the binding is dependent on the amount of glucocorticoid [13] In addition, this binding results in exhibition of anti -in ammatory effects through inactivation...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... This is the first report suggesting that this metal binding domain is functional within APP C99 causing oligomerization with the biochemical consequence of inhibiting c-secretase cleavage This ... in [6]) In vitro c-secretase assays have been essential in elucidating the mechanism of inhibitors [7–9], the structure of active c-secretase complexes [10,11], and have aided in the finding of ... Since a functioning zinc-binding domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the oligomerization state of C101-3FLAG Therefore, the molecular mass of...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
... checkpoint ATM, a member of phosphoinositide kinase-related kinase (PIKK) which is involved in DNA repair [13] ATM activation, in turn, leads to the phosphorylation of p53, thereby blocking its interactions ... Penicillin-Streptomycin (100 U/ml) and 10% heat-inactivated FBS All cells were obtained from ATCC and maintained in a humidified atmosphere of 5% CO2 and 95% air 10 mg of DCQ was dissolved in ml of ... the greatest increase in the Pre-G1 population (Figure 3B), further confirming the higher drug sensitivity of this cell line There is no dose-response toxicity by DCQ in normal intestinal cells...
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Báo cáo y học: " Detrimental effects of albuterol on airway responsiveness requires airway inflammation and is independent of b-receptor affinity in murine models of asthma" pdf
... (n = 10 in each group) Results of scoring of PAFS stained histological sections of lungs; no statistical difference was found between groups Results of scoring of CCSP staining; no statistical ... b2-agonists have been shown to induce increased production of pro-inflammatory IL-13 in Th2 cells from asthmatic patients in vitro, which was suggested to be independent of the isomer of albuterol[37] ... albuterol[37] In this context, it is interesting to note that in our study the single isomer (R)-albuterol did not significantly induce inflammatory cytokines However, when (S)-albuterol was present in...
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Báo cáo y học: "Acute mucosal pathogenesis of feline immunodeficiency virus is independent of viral dose in vaginally infected cats" pptx
... observations raise new questions about defining correlates of protection and how they could be more clearly distinguished in the context of future vaccine trials [17,18] Further, as animal model vaccine ... of initial viral dose on the presence and severity of mucosal pathogenesis is unclear, in particular when the route of infection is via the reproductive mucosa Therefore, a better understanding ... were significant This is in spite of significant differences in cytokine production when comparing viremic and non-viremic cats Further, tissue specific cytokine responses in LPL were evident...
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Báo cáo y học: "Apoptosis resistance in HIV-1 persistently-infected cells is independent of active viral replication and involves modulation of the apoptotic mitochondrial pathway" pot
... Apoptosis resistance is independent of the magnitude of viral replication Apoptosis resistance is independent of the magnitude of viral replication A) Jurkat and J1.1 cells were incubated in the ... resistance of HIV persistenly-infected cell lines involves modulation of the mitochondrial pathway In order to dissect the mechanisms involved in this protective effect, uninfected or persistently-infected ... can influence the susceptibility to apoptosis [19] These data suggest that apoptosis resistance in persistently-infected cell lines is independent of the magnitude of viral replication Apoptosis...
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Báo cáo y học: " Induction and effector phase of allergic lung inflammation is independent of CCL21/CCL19 and LT-beta
... cascade of cytokines and chemokines necessary for tissue eosinophilia without involvement of draining lymphoid tissues We studied the induction of lung allergic inflammation in mice lacking LTβ ... within nearly all tissues, presumably serving a kind of sentinel function Inflammatory stimuli such as TNF and endotoxin are extremely effective in inducing resident tissue antigen presenting ... production of the cytokines IL-4 and IL-13, which in turn induced epithelial production of the eosinophil chemokine eotaxin [28] Since local Th2 cell production of cytokines has also been implicated in...
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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf
... characterization of a JC virus pentanucleotide repeat element binding protein: cellular nucleic acid binding protein Virus Res 58, 73–82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular ... Blue stained gel Arrow on the right indicates GST-CNBP; asterisk on the right indicates GST protein Fig S5 In vitro phosphorylation assays in presence of different concentrations of H-89 In vitro ... effective tool for customizing the action of other factors and their upstream signalling systems In the case of c-myc gene, this mechanism is proposed to proceed through the binding of trans factors,...
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Báo cáo khoa học: p53-induced inhibition of protein synthesis is independent of apoptosis pdf
... growth inhibitory effects of the activated tumour suppressor protein Detailed analysis of the kinetics of the inhibition of translation has shown that this effect begins within 2–4 h of activating ... extent of phosphorylation of the inhibitor of polypeptide chain initiation factor eIF4E, 4E-BP1, is reduced following activation of p53 in the Val135 cells and that this results in sequestration of ... the inhibition does not involve other pathways that are inactivated in the presence of Epo Discussion The tumour suppressor protein p53 is activated in cells by a number of stresses, including...
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Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx
... recycling [32] Binding of visual arrestin to rhodopsin requires GRK phosphorylation of rhodopsin Several other studies have confirmed the role of GRK phosphorylation in binding of b-arrestin to ... this concentration was maintained during radioligand incubation Data were analysed using GraphPad PRISM software Results Visualization of agonist-mediated internalization of CRFR1 The C-terminally ... involvement of clathrin-coated pits in the agonist-induced internalization of the CRFR1 125 PKA and PKC inhibitors not abolish agonist-induced CRFR1 internalization As CRFR1 activation is thought...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... boundaries of independent folding domains in the a subunit of tryptophan synthase, a b ⁄ a barrel protein Protein Sci 8, 1200–1209 Jasanoff A, Davis B & Fersht AR (1994) Detection of an intermediate in ... 000 of 87 of 68 of 87 11 of 87 38 of 87 < 1.1 < 1.5 2.3 13 44 arginine of the new epitope, and removed the fragment of the PRAI (ba)8-barrel corresponding to a6(ba)7)8 The remaining fragment of ... space, as in filamentous phage display), increasing the likelihood of correct folding in a reducing environment while minimizing the risk of proteolytic degradation The association of plasmid...
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Báo cáo sinh học: " Respiratory syncytial virus-induced acute and chronic airway disease is independent of genetic background: An experimental murine model" pot
... strains of mice Therefore characterizing and establishing a comprehensive model of acute and long-term RSV disease in C57BL/6 is essential to further understanding the pathogenesis of RSV disease ... differences during the acute phase of the disease, there were no significant distinctions in the pattern of cytokine responses, lung inflammation or clinical manifestations of disease, and both strains ... dynamics of inflammatory infiltrates, airway dysfunction (AO and AHR), and inflammatory cytokines during the long-term phase of RSV-induced disease, up to 77 days after primary infection in both...
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Life in the city is full of activityLife in the city is full of activity. docx
... and it is full of noise Hundreds of sight-seers tourists and others visit many place of interest in the city while businessmen from various parts of the world arrive to transact business The ... business centers With the coming of night, another kind of activity begins The streets are now full of colorful lights The air is cooler and life becomes more leisurely People now seek entertainment ... visit the cinemas, parks, and clubs, while others remain indoors to watch television or listen to the radio Some visit friends and relatives and some spend their time reading books and magazines...
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Ultrasonography in In Vitro FertilizationRoger A. PiersonDepartment of Obstetrics, Gynecology pptx
... some promising new areas where imaging has the potential to enhance our understanding in assisted reproduction The essentials of ultrasonography in IVF are in monitoring the course of ovarian ... protocol and the number of oocytes desired and clinical assessment of the risk of ovarian hyperstimulation will dictate increasing or decreasing daily doses of gonadotrophins It is important to note ... develop during ovarian stimulation protocols This information is equally important when assessing the risks of ovarian hyperstimulation In the past, follicular growth rates during induced cycles...
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Báo cáo y học: "Induction of arthritis by high mobility group box chromosomal protein 1 is independent of tumour necrosis factor signalling" ppt
... given a single intraarticular injection of μg pHMGB1 into a knee joint This dose has been established in previous experiments to induce arthritis [4] Intraarticular injection of HMGB1 into healthy ... lower in comparison with other mouse strains tested [4] In the present study we observed mild synovial inflammation in 32% to 39% of mice upon intraarticular injection of HMGB1, which is in accordance ... irrespective of the presence or absence of the TNFα gene These results indicate that HMGB1 induces joint inflammation in vivo independently of the TNFα pathway Figure TNFα-/- induction by high Arthritismice...
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báo cáo khoa học:" Quality of life associated to chronic pelvic pain is independent of endometriosis diagnosis-a crosssectional survey" pot
... groups Discussion In this study, we demonstrated that the intensity of pelvic pain is inversely correlated with alteration in the quality of life of patients with CPP (higher scores of pelvic pain ... symptom As a distinctiveness of our paper, we investigated the effect of endometriosis itself in quality of life of patients that presented with CPP Pain is one of the major concerns of patients ... decrease in quality of life in two domains-psychological and environmental) Furthermore, we showed that the presence of endometriosis as a cause of CPP is not an independent factor for modifying quality...
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Báo cáo y học: " Resolution of LPS-induced airway inflammation and goblet cell hyperplasia is independent of IL-18 J Foster Harris1, Jay Aden1, C Rick Lyons2 and " doc
... time points following LPSinstillation may have initiated production of VEGF at d 6– post instillation Figure with IL-18 neutralizing in BALF of rats instilled and injected Inflammatory cell influxantibody ... levels found in saline instilled controls by d (Fig 2A) We determined the levels of chemokines and cytokines in the BALF that have been reported to be important in recruiting and activating inflammatory ... after injury in various systems [33] and is important in the resolution of inflammation in some tissues, because this process is characterized by enhanced angiogenesis [34] The early production of...
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Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx
... opportunity of in vivo assessment of tumor response and the possibility of determination of potential predictive factors, which may influence clinical decision making in the future However, this potential ... prognosis in canine mammary tumors [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corresponding adjacent tissues, have been similar [8] In ... chemotherapy is not routinely administered to dogs, as mammary gland conservation is of limited value An option would be to analyze tumor response to chemotherapy in vitro Increasing evidence indicates...
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Báo cáo y học: "Tumor necrosis factor alpha-dependent aggrecan cleavage and release of glycosaminoglycans in the meniscus is mediated by nitrous oxide-independent aggrecanase activity in vitro" pptx
... degradation [21] In order to investigate the influence of TNFα on the meniscus we present a bovine in vitro model that allows the isolation of meniscal tissue explants of defined geometry and ... den: Role of interleukin-1, tumor necrosis factor alpha, and interleukin-6 in cartilage proteoglycan metabolism and destruction Effect of in situ blocking in murine antigen- and zymosan-induced ... fragment in meniscal tissue by immunostaining in TNFα-treated explants, while it was almost non-detectable in control tissue This is another strong indicator for aggrecanase involvement, according...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25