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analysis of the cattle liver proteome by high sensitive liquid chromatography coupled with mass spectrometry method

Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Báo cáo khoa học

... numbers The left inset of (B) shows the spectra of the four main peaks obtained by the diode array detector The right inset of (B) reports the SDS/PAGE of phycobilisomes recorded by scanning the ... 6803, together with the determination of their molecular mass, has successfully been achieved [14] by the combined use of HPLC coupled on-line with a mass spectrometer equipped with an electrospray ... calculated by using the Prot Parameter tools in the EXPASY program The SDS/PAGE molecular masses were deduced by the marker used in the electrophoresis reported in the right inset of Fig 1B Molecular masses...
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Economic Analysis of the House Budget Resolution by the Center for Data Analysis at The Heritage Foundation pot

Economic Analysis of the House Budget Resolution by the Center for Data Analysis at The Heritage Foundation pot

Cao đẳng - Đại học

... interaction with the fiscal revenues and spending variables The feedback effects further increase or decrease the longerterm impact of the policy, providing a quantitative picture of whether the economy ... so, the extensive margin (expanding the labor force).24 The change in the labor supply variables were adjusted by the macro-labor elasticity of two, which is a middle estimate of the ranges The ... underlying the adjusted GI long-term baseline in the years 2022 to 2041 trend to the percentage change in the series applied to the value of the previous quarter beginning with 2021 Quarter There...
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Báo cáo y học:

Báo cáo y học: " Expansion of the human mitochondrial proteome by intra- and inter-compartmental protein duplication" ppsx

Báo cáo khoa học

... Ca2+, as the binding site is created upon the formation of a complex with the E2 subunit of the PDH complex and requires the lipoyl groups of E2 [44] Nevertheless, the calcium-dependence of PDP1 ... Biology 2009, of eutheria) The duplication pattern of post-translational regulators of the PDH complex differs from that of the complex itself The inactivating phosphorylation of the PDH complex ... signal coinciding with the gene duplication event could provide the rationale for the retention of the duplicated gene As the change of localization alters the role of a protein in the cell, it could...
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A text analysis of “the 2007 commencement speech by bill gates at harvard university ” and “the 2014 commencement speech by bill and melinda gates at stanford university” on the de beaugrande framework

A text analysis of “the 2007 commencement speech by bill gates at harvard university ” and “the 2014 commencement speech by bill and melinda gates at stanford university” on the de beaugrande framework

Ngữ pháp tiếng Anh

... non-verbal language, the tone of voice, the idea, the cadence of the words and the rhythm of the sentences and so forth However, this thesis provides a very modest analysis on the success of two commencement ... other six items With ―Coherence‖ the importance of the relations between the concepts, in a text words, is comprehended; with ―Intentionality‖ the necessity of the leadership of the aim and the ... ―Informativity‖ the vitality of getting the new message correctly and being capable of differentiating the order of the importance of these messages, with ―Situationality‖, maybe the primary one, the context...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

Báo cáo khoa học

... sites within the MGP coding sequence (Fig 2) Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns and are of phase I while intron is of phase ... this study, we present the molecular organization of the rst nonmammalian MGP gene and the functional analysis of its promoter We identied a region within the rst 70 bp of the xMGP promoter that ... obtained with the )70/)36TATALUC was approximately sevenfold higher than the one obtained with the )134/)36TATALUC, suggesting the presence of negative regulatory elements located in the region...
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Báo cáo y học:

Báo cáo y học: "Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry." ppsx

Báo cáo khoa học

... China) The cartridge was firstly conditioned with ml of methanol and then equilibrated with ml of water After sample was loaded, the cartridge was washed with 2.0 ml of methanol (30%) The analyte ... The comparison of the spectra of quasi-MS/MS and of quasi-MS/MS/MS may yield the quasi-third order precursor ions The quasi-MS4 spectra may be obtained through collision of the ions with argon in ... isonaringin We found that the mass spectrometry fragmentation behaviors between the two pairs of flavanone 7-glycoside isomers were very different, while the within pair mass spectrometry behaviors...
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Báo cáo y học:

Báo cáo y học: "Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry" pptx

Báo cáo khoa học

... Lin M: The study on the constituents of the roots of Polygonum multiflorum Thunb Zhongcaoyao 1993, 24:115-118 Zhang ZG, Lu TS, Yao QQ: Study on the anthraquinone components of the roots of Polygonum ... solid sample prepared according to the method Stability of the method was determined with the sample solution after 0, 2, 4, and 12 hours in a single day and for further one and two days Data processing ... Multiflori; HPLC: high performance liquid chromatography; MS: mass spectrometry; THSG: 2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucopyranoside Acknowledgements The project was supported by the Faculty...
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Simultaneous determination of sildenafil, vardenafil and tadalafil as forbidden components in natural dietary supplements for male sexual potency by high performance liquid chromatography–electrospray ionization

Simultaneous determination of sildenafil, vardenafil and tadalafil as forbidden components in natural dietary supplements for male sexual potency by high performance liquid chromatography–electrospray ionization

Hóa học - Dầu khí

... low LOD and LOQ with UV detection Hence the UV detection method can be used for conventional analysis of these compounds even without mass spectrometry However, the MS LOD of these compounds ... method to determine these components Concerning the analysis of these compounds, there are a few reports that introduced the strategy for the determination of sildenafil by the widely used HPLC ... constructed by plotting the average peak areas of the standard compounds against the corresponding concentrations The limit of detection (LOD) of UV detection and MS–SIR was evaluated as the mass giving...
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Báo cáo

Báo cáo "DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID CHROMATOGRAPHY PART 2. SEPARATION AND QUANTIFICATION SULFAGUANIDINE, SULFAMETHOXYPYRIDINE, SULFADOXINE AND SULFAMETHOXAZONE IN ANIMAL FOODSTUFFS AND FOOD PRODUCTS " pdf

Báo cáo khoa học

... improved methodology for the determination of sulfonamide residues in meat Journal of Chromatography A 1159 (2007)233-241 SUMMARY DETERMINATION SULPHONAMIDES RESIDUES IN FOOD BY HIGH PERFOPvMANCE LIQUID ... Dunnbier Gudrun Massmann, Asaf Pekdeger - Quantitative determination of three sulfonamides in environmental water samples using liquid chromatography coupled to electrospray tandem mass spectrometry ... (2004) 2329 Che-Yi Lin, Shang-Da Huang - Application of liquid- liquy microextraction and high performance liquid chromatography for the determination of sulfonamides in water Analytica Chimica Acta...
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Báo cáo y học:

Báo cáo y học: "Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery" docx

Báo cáo khoa học

... using online capillary liquid chromatography coupled with tandem mass spectrometry (MS/MS) using an LTQ-Orbitrap XL mass spectrometer (Thermo Scientific, San Jose, CA) The chromatography conditions ... compatible with MS-based shotgun proteomics? 2) What is the composition and extent of contamination by saliva of the exudate proteome? 3) Can the differential abundance of protein within exudates ... relative amounts in the whole saliva samples compared to exudate samples To further elucidate the composition of the exudate proteome, we compared it to the proteome derived from the epithelial cells...
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studying histamine in seafood products by high performance liquid chromatography

studying histamine in seafood products by high performance liquid chromatography

Tổng hợp

... peak ascribed to the excess of benzoyl reagent decreased, without the loss of the peak areas of the analytes Another advantage of using benzoyl chloride is the simple way to stop the derivatisation ... exceed the safe limits (50 mg/kg) at the end of the life of the experiment 16 Figure 7: Mean of histamine concentrations in tuna samples stored at 4oC by day of storage Figure 8: Mean of histamine ... suggested by Earola et al (1993), could not totally remove the excess of dansyl chloride As a result, low amounts of the by- products can still be detected in the chromatogram and the peak of histamine...
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Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography–electrospray tandem mass spectrometry

Highly sensitive and accurate screening of 40 dyes in soft drinks by liquid chromatography–electrospray tandem mass spectrometry

Tổng hợp

... preconditioned with 5.0 mL methanol followed by 5.0 mL acidified water The samples were loaded through the cartridges at a rate of less than 3.0 mL/min The cartridges were then rinsed with 5.0 mL of 15% ... dard solution of these dyes, and their optimum values are listed in Table 2.4 Method validation Quantitative analysis was carried out by the external standard calibration method The calibration ... prepared by appropriate dilution of intermediate mixed standard solutions in water to concentrations between 0.0015 and 10 ␮g/mL The sensitivity of the method was evaluated by estimating the limit of...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx

Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx

Báo cáo khoa học

... actin in the nucleus has been demonstrated by immunogold staining with partial digestion of the surface lamina of the nuclear matrix to allow penetration of the gold particles into the nuclear ... accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation Subcellular fractions of rat liver were ... underneath the inner nuclear membrane and function to maintain the nuclear architecture [3] The majority of vimentin may be bound to the nuclear lamina from the outside the nucleus [18] The presence of...
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An analysis of the inaugural address by g w bush in the u s president election 2004 from a perspective of discoure analysis

An analysis of the inaugural address by g w bush in the u s president election 2004 from a perspective of discoure analysis

Khoa học xã hội

... All in all, the above analysis of Themes in this speech proves the assumptions made earlier Thematization highlights the implicit ideology of the author the thematic concern to the freedom cause ... say Mr Bush is the addresser with absolute power: as the Head of the State and as the Commander -in -Chief of the Army His authority is unlimited over the nation The audience of the speech; meanwhile, ... point of departure of the message denoting what the clause is concerned with Rheme meanwhile is the part where Theme is developed and it lies in the remainder of the clause Normally, the choice of...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

Báo cáo khoa học

... delivery to the liver and induces susceptibility to atherosclerosis The regulatory effect of KLF4 on SRBI reveals a novel pathway to elucidate the mechanism of SR-BI in the development of atherosclerosis ... of oxLDL on the expression of KLF4, the potential reason may be the deficiency of a corresponding ligand–receptor interaction Here, the changes in the SR-BI response to oxLDL were consistent with ... to the binding element of the SR-BI promoter region The ChIP assay was used to detect the binding of KLF4 to the SR-BI promoter The cross-linked protein-DNA complexes were immunoprecipitated with...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Báo cáo khoa học

... outside of the nuage in the cytoplasm of the migrating PGC FCS analysis revealed that Olvas GFP diffused with a diffusion constant of 7.0 lm2ặs)1 (Fig 3A) During the measurement of the PGC, the 10 ... and to measure the mobility of the components in the PGCs The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised for observation by microscopy and ... protein in the HeLa cell [24], suggesting the existence of some interactive cellular partner FRAP analysis of the nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part of the single...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx

TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx

Tự động hóa

... avoiding the use of highly flammable or explosive gases in the laboratory The oxygen is added either in the spray chamber or using a T-connector before the torch When oxygen is added to the plasma, the ... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some ... match the detection capability of the ShieldTorch System in high purity matrices This application note deals only with the use of cool plasmas for the analysis of organics, and this technique...
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Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

Báo cáo khoa học

... coincides with the expected molecular mass of the ternary complex ( 110 kDa) The TIPRL peak fractions indicated in the bottom of the left panel were fractionated by ion exchange chromatography The ... binding of the third protein without displacing the one that was previously associated with the complex was interpreted as an evidence of simultaneous binding to PP2Aca The results of these in ... regulator of the rapamycin -sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator of the activity of type 2A...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx

Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx

Báo cáo khoa học

... interact with the antitoxin The possible effects of the mutations on the stability and structure of the protein were analysed by inmunoblotting and CD, respectively The potential of the Kid mutants ... 1A) Substitution of these residues by glycine abolished interactions with the bases without disturbing the structure of the flexible region in which they are located The fact that these substitutions ... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the different levels of dilution 4984 CD...
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