an important player in the dna damage response
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... by introducing an NsiI site at the translation start point of galA and by thencloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking ... galacto-oligosaccharides.ACKNOWLEDGEMENTS The authors thank Simon Flitter for the identification and isolationof the galA containing phage clones and Matthew Illsley for the analysis of a-L-1,5-arabinofuranosidase activity in the ... demonstrates the important role of GALA in the degradation of both linear andL-arabinose-substitutedgalactan side chains of pectin. This is in agreement with aprevious study in which the synergy...
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Báo cáo khoa học: NtKTI1, a Kunitz trypsin inhibitor with antifungal activity from Nicotiana tabacum, plays an important role in tobacco’s defense response pot
... indicated by an arrow in the right lane. (B) In vitrobovine trypsin inhibition by the recombinant NtKTI1 protein (opencircles). Soybean trypsin inhibitor (triangles) was assayed in parallelas ... 4B).Tobacco plants overexpressing NtKTI1 showenhanced resistance to R. solani infectionTo evaluate the in planta role of NtKTI1 in defense,sense and antisense lines under the control of the cauli-flower ... trypsin inhibitor with antifungal activityfrom Nicotiana tabacum, plays an important role in tobacco’s defense response Hao Huang*, Sheng-Dong Qi*, Fang Qi, Chang-Ai Wu, Guo-Dong Yang and Cheng-Chao...
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Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt
... itsNH2-terminal region, as well as an Mre11-bindingdomain and an ATM-binding domain in its COOH-terminal region (Fig. 1). FHA and BRCT domains areoften present in eukaryotic nuclear proteins involved in ... MRN foci in cells exposed to IR, led to the hypothesis that the MRN complex functions in the sensing of DNA strand breakage and in the activationof the ATM-dependent DNA damage signaling path-way ... substrates in the ATM-depend-ent DNA damage signaling pathway [10,11,39].Given that ATM is a central player in the cellular response to DNA strand breakage, it is important tounderstand the mechanisms...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... of the N-terminal region on the molecular surface. In the wild-type enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268. The oxygen of the main chain ... conformational changeson the surface. On the other hand, anionic surfactants suchas SDS and LAS bind to the surface regions of protein, and the surface regions would be unfolded and removed from the core ... from the amino acid sequence alignment of ALP I and the neutralsubtilisins. These candidate residues did not occur at the corresponding positions of the alkaline subtilisins. Fur-thermore, on the...
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Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc
... spans the entire thicknessof the inner retina. The Mu¨ller cell constitutes an ana-tomical link between the retinal neurones and supportstheir activities by exchanging molecules between the other ... RPE65c and GS staining (B3), and 4¢,6-diamidino-2-phenylindole (DAPI) staining (B4), respectively. (B5-8) High magnificationimages of the boxed area in (B4) showing RPE65c staining (B5), GS staining ... contribute toproviding chromophore to melanopsin to maintaintheir light sensitivities. The expression and function ofRPE65c in other cell types in the retina remains to beinvestigated.Earlier...
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf
... linked hairpins in the membrane[10]. Importantly, the ion-binding site is not conserved in hairpin one. If one assumes the same number oftransmembrane helices in V0and F0, the rotor ofeukaryal ... (lanes 6, 8 and 9), andindividual subunits were detected by silverstaining (lane 6) or immunoblotting using an antibody against subunit c1(lanes 7 and 8)or subunit c2 ⁄ 3(lane 9). The antibodyagainst ... handedness, and twoof the rings face in the opposite direction in the membrane to the other two, forming the same patternas in the AFM surface representation of Fig. 2A. Bycomparison with the...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... rectangles, b-strands as arrows. ÔdÕ indicates residues in the dimer interface and ÔtÕ residues in tetramer interface.Residues lining the active site cavity are underlined. Residues binding ... of the corresponding intermediate in transketolase [37] and the model derived for ScPDC [38] (Fig. 6). In the immediatevicinity of the modelled a-carbanion/enamine, there are anumber of invariant ... by amino acids with a largehydrophobic side chain. Furthermore, there are significantstructural differences between ZmPDC and IPDC on the one hand, and ScPDC on the other hand involving the C-terminal...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... patterning in earlyembryonic development and in the antimicrobial immune response in larvaeand adults. We have investigated the function of Spa¨tzle in a lepidopteraninsect, Manduca sexta, in ... the protein was transferred to apoly(vinylidene difluoride) membrane and stained with 40%methanol containing 0.025% Coomassie Brilliant BlueR-250. After destaining with 50% methanol, the bandcorresponding ... gene. In the clade includingSpa¨tzle-1, the branch lengths are noticeably longer and the bootstrap values are lower than in the other cladescontaining Spa¨tzle-2 to Spa¨tzle-6, indicating...
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Báo cáo khoa học: A single mismatch in the DNA induces enhanced aggregation of MutS Hydrodynamic analyses of the protein-DNA complexes pot
... protein incubated with increas-ing concentrations of either ATP or ATPcS was centri-fuged followed by assaying the protein concentrations in the supernatant as well as the pellet. In the set ... con-taining ATPcS, the entire protein sample was recov-ered in the supernatant (Fig. 5A) and no protein wasdetected in the pellet fractions (data not shown). In the same conditions the ATP ... have investigated the changes associ-ated with the molecular aggregates of mismatch repairproteins MutS, MutL in relation to their interactionwith mismatch containing DNA and the ongoing ATPhydrolysis....
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Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot
... cellspeci®c anticancer therapy [29], we encountered largedifferences in the responses of the various human cancercell lines to the same ODN. We have examined this effect in detail and extended the analysis ... is inactive in the in vitro RNaseH assay, or that some other enzymatic activity is measured. The detection of increased activity in the transfectantsoverexpressing the coding region of the ... as the ODN h ybridizes to the center of the input target RNA. The asterisk indicates the input targetRNA; the arrow indicates the digested product bands. The amount ofdigested product obtained...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... pUZ8002 bearing the cosmidwith oriT and the S. coelicolor DdesA mutant, single cross-over exconjugants were screened for kanamycin resistance.Kanamycin-resistant colonies were isolated and analysed ... As the DdesA mutant is apramycin resistant, the kanamycinresistance marker was cloned into an XbaI site of the newconstruct (pSKdesA). A 5723-bp XhoI+NotI fragmentcontaining the desA gene and ... procedures), and the DdesA mutation was veri-fied in one of the mutants by PCR and Southern blotanalysis. A 1462-bp PCR band corresponding to the extended resistance cassette was found only in the mutant...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... narrower line-widths than anyother residues in the protein. This indicates that the PEST sequence is an unstructured, highly mobileregion within SOCS3. The PEST sequence is an insertion in the SH2domainAnalysis ... C-terminalsuppressor of cytokine signalling (SOCS) box. SOCS3 and the related pro-tein, cytokine-inducible SH2-containing protein, are unique among the SOCS family of proteins in containing a region of mostly ... the kinase inhibitory region and the entire extended SH2subdomain form a single a-helix. The mapping of the true SH2 domain,and the location of its C terminus more than 50 residues further...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
... mRNA insta-bility and the involvement of the ARE in this process[10,11]. In addition, another destabilizing sequence wasidentified within the coding region of IFN-b mRNA [12,13].Whereas the independent ... 1A). We then analysed, by Northern blot, the induc-tion and decay of IFN-b transcript in the same conditions.AsshowninFig.1B,IFN-b mRNA was detectable 4 hafter the beginning of the infection, ... encoding the HA epitope wasinserted at the end of the IFN-b coding sequence todistinguish the products resulting from the expression of the DNA constructs and the endogenous gene (Fig. 2). Theseconstructs...
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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx
... beenproposed as a Mg2+-binding ligand in a product com-plex, in addition to its initial role in direct ligation of the sugar OH group in an analogous manner to thatfound in the DNA ⁄ RNA polymerase ... identified Lys19 in the Haemo-philus ducreyi CNS, and Lys21 and Arg12 in CNSfrom Escherichia coli (Fig. 1) as important catalyticresidues, indicating a role in binding the nucleotideinto the active ... of the purifiedF192A and F193A mutant enzymes were obtained in the same way as the wild-type enzyme (Table 1). The results support the role of these residues in bindingNeu5Ac, because the mutants...
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Báo cáo khoa học: A unique tetrameric structure of deer plasma haptoglobin – an evolutionary advantage in the Hp 2-2 phenotype with homogeneous structure pot
... dolphins and whales are the closestdivergences before the ruminants, we further examined the size of the a-chain in whales and dolphins as wellas other ruminants (cattle and goat) to determine the possible ... time of the tandem repeat evolution in deerHp. Interestingly, the inserted panel of Fig. 10 showsthat the a-chains of all the ruminants tested are the a2type, except for dolphins (n = 5) and whales ... linkages within the a-chain (Cys34 and68 and Cys93 and 127), and the one at the C-terminalregion (Cys131) cross-links with the b-chain (Cys105) toform a basic a-b unit. Under such an arrangement,Cys15...
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