... sites from proinflammatory to anti-inflammatory [55-57] In a murine pulmonary fibrosis model, MSCs inhibited bleomycin-induced inflammation and fibrosis within the lungs This was shown to be due ... used in vivo for enhancing the engraftment of other tissues (for example, hematopoietic stem cells) MSCs can support hematopoiesis through the secretion of cytokines and have the capacity to maintain ... Growth factors that have regulatory effects on MSCs include members of the transforming growth factor-beta (TGF-β) superfamily, the insulin-like growth factors, the fibroblast growth factors, the platelet-derived...
... transplantation into rats, confirming that these cells were seeded into the liver tissue by BMDC and can modify cell phenotype mimicking the one of resident cellsinthe host tissue Insults trigger the release ... Hematopoietic Cells as Hepatocyte Stem Cells: A Critical Review of the Evidence Hepatology 2006, 43:2-8 49 Jang YY, Collector MI, Baylin SB, et al: Hematopoietic stemcells convert into liver cells ... increase inthe cytoplasmic concentrations of b-catenin results in its translocation from the cytoplasm tothe nucleus Once inthe nucleus, b-catenin acts as a co-activator to stimulate the transcription...
... fresh encouragement tothe endeavors to elucidate the immunomodulatory effects of MSCs in rheumatic diseases [1] Given the apparent difficulties in recapitulating thein vitro effects in vivo, however, ... MSC, the first studies in human autoimmune disease are already appearing [10,11] Not unexpectedly, however, the clinical effects are not coherent Concluding remarks The mechanisms underlying the ... of MSCs to modulate immune responses therefore probably depends, in part, on the composition of the starting population Despite our incomplete understanding of the mechanisms underlying these divergent...
... this inflammatory and catabolic cascade and demonstrated that curcumin (6) has the capacity to block the action of pro-inflammatory cytokines inthe joint thus disrupting the inflammatory cycle inhibit ... (1) lead tothe production and accumulation of high levels of pro-inflammatory cytokines (2) inthe joint These trigger the production of additional pro-inflammatory cytokines which induce genes ... curcumin inhibits IL-1β induced apoptotic and inflammatory signalling pathways, promoting co-culture induced chondrogenesis Curcumin suppresses IL-1β-induced apoptotic and inflammatory responses in...
... performed using the corresponding inhibitors They included 1-MT (1 mM), an inhibitor of IDO enzymatic activity, INDO (5 μM), an inhibitor of PGE2 synthesis, L-NAME (1 mM), a specific inhibitor of ... proinflammatory cytokine IL-6 Interestingly, the UC-MSCs could downregulate the IL-6 production inthe transwell but not the cell -to- cell contact system (Figure 3c) both in single time point and in dynamic ... cellsin vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs inthe joint of the CIA...
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... bone marrow cells facilitates expansion of marrow-derived mesenchymal progenitor cells and prevents their differentiation into osteoblasts J Bone Miner Res (12):1943-1956 64 Akino K, Mineta T, Fukui ... expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological...
... H.O., and T.N.) independently made a diagnosis using the μm sections stained with haematoxylin and 2 Immunohistochemical Staining The neuroendocrine nature of tumors was confirmed with the three representative ... those in SCLC inthe region (y>3.84, z>3.84) on the y-z plane and those in LCC inthe region (x>3.84, y>3.84) Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... search settings used, we identified significant proteins as follows: LCNEC contained a total of 1,124 proteins including 410 unique, 168 inthe overlap only between LCNEC and SCLC, 93 inthe overlap...
... infarction in each brain slice was measured using Image J analysis software (version 1.6 NIH) The infarct volume was indirectly determined by subtracting the volume of intact tissue inthe ipsilateral ... many inflammation cytokines and chemokines were released into peripheral blood including vascular cell adhesion molecule 1, p-selectin, CXCR4 and SDF-1, which promote the adhesion of MSCs tothe ... the endothelium or induce the migration of MSCs tothe ischemic tissue inthe brain [20-22] However, in previous studies, it has been demonstrated that the transplanted cells may be detained by...
... infarction in each brain slice was measured using Image J analysis software (version 1.6 NIH) The infarct volume was indirectly determined by subtracting the volume of intact tissue inthe ipsilateral ... many inflammation cytokines and chemokines were released into peripheral blood including vascular cell adhesion molecule 1, p-selectin, CXCR4 and SDF-1, which promote the adhesion of MSCs tothe ... the endothelium or induce the migration of MSCs tothe ischemic tissue inthe brain [20-22] However, in previous studies, it has been demonstrated that the transplanted cells may be detained by...
... growth factor combination [HGF and fibroblast growth factors and (FGF1 and FGF4)] to induce mouse ES cellsto develop into functional hepatocytes These factors, named HIFC (hepatic induction factor ... compared with the expression level in human hepatocytes (reference sample) Mesenchymal- to- epithelial transition in AT-MSC-Hepa Although AT-MSCs indeed differentiate into hepatocyte-like cellsin vitro, ... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver...
... share the same embryologic origin as the uterus They have the capacity to undergo dynamic endocrine-induced changes during the menstrual cycle, including cell growth and regeneration, in order to ... cells induced for myogenic differentiation 1A) Dystrophin expression in muscle control and inthe induced hFTs cells 1B) Skeletal myosin expression in muscle control and inthe induced hFTs cells ... 200) Discussion The possibility of using stemcells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular...
... transplanted cells into the host tissue [3] Interestingly, while stem cell therapy, including bone marrow-derived mesenchymalstemcells [15-17], embryonic stemcells [14] and endothelial progenitor cells ... extensively investigated inthe treatment of strokeinexperimental studies and, to a lesser extent, in humankind, the use of adipose-derived mesenchymalstemcells (ADMSCs) for the treatment of stroke ... tested the hypothesis that ADMSC therapy is safe and effective in limiting the size of brain infarct and improving neurological function in a rat model of acute IS We further investigated whether intravenous...
... embryonic stems cells and bone marrow-derived mesenchymalstem cells, adiposederived mesenchymalstemcells (ADMSCs) have the distinct advantages of being abundant, easy to obtain with minimal invasiveness, ... preservation of heart function in DCM rats Accordingly, our finding strengthens the finding of previous study [20] Another important finding inthe current study is that combined therapy with ADMSCs and ... Stress To determine whether inflammatory cells were up-regulated in LV myocardium on day 90 following DCM induction, immunohistochemical staining for detection of CD40-positively stained cells...
... lung injury through up-regulation of Page 10 of 13 anti-inflammatory cytokine IL-10 [25] Accordingly, in addition to reinforcing the findings of previous studies [17,25,28,32], the results of the ... (Figure 1A) On the other hand, RVSBP was notably lower in groups and than in group 2, and remarkably higher in group than in group (Figure 1B) These findings indicate that IR injury intheexperimental ... staining identified substantially higher number of infiltrated CD68+ cells (inflammatory cells of macrophages) in injured lung parenchyma in IR group than in normal control Our findings, therefore,...
... antibodies were then added tothe resuspended cells for 30 at 4°C inthe dark Positive cells were then detected on flow cytometers Immunocytochemistry of MSCs and TH expression in PC12 cellsTo identify ... co-culture of PC12 cells and MSCs Inthe MSCs alone, thecells showed an elongated fibroblast shape (Fig 3A) Inthe PC12 cells alone, thecells showed modest growth and tended to grow in small clusters ... cell group, and the combined MSC + PC12 cell group The 5 cells were seeded at 1.5 × 10 cells of each cell type (3 × 10 total inthe combined group) onto 60 mm dishes and grown in DMEM supplemented...
... attributable tothe introduced canine UCB-MSCs or to an endochondral sequence, the injected canine UCB-MSCs injected might play a role in osteoconduction inthe graft site The results of the present ... progresses toward the center of the graft Remodeling of the graft takes months to years, depending on the length, and might never be complete [24] Inthe present clinical case, bone lysis and instability ... osteoprogenitor cells, bone remodeling and graft resorption that occur together with host bone ingrowth into and onto the porous coralline microstructure or voids left behind during resorption...
... during in vitro culture [4] These findings suggested the presence of mesenchymalstem cell-like cells according tothe standard criteria for MSCs from the International Society for Cellular Therapy ... cells For group V, the injured site was exposed by dorsal laminectomy and 150 μL of PBS was injected into the spinal cord at locations to depths of mm using a 30 gauge needle (middle of the injury ... effective in canine SCI model, there was little histological evidence of spinal cord tissue regeneration [22] In this study, we examined whether canine ASCs could survive and integrate into neural cells...