... pinnata, syn Bryophyllum calycinum, Bryophyllum pinnatum, loài bản địa của Madagascar C y sống đời có khả sinh sản sinh dưỡng tốt, đặc điểm chung của nhóm Bryophyllum thuộc chi Kalanchoe C y sống ... (9-decenyl) phenanthrene (I) (undecenyl) phenanthrene (II) Năm dẫn xuất Bufadienolide: Bryophyllin B, Bryophyllol, Bryophollone, Bryophollenone, Bryophynol 18 oleanane α ψ-taraxasterol Alpha β-amyrins ... c y (3 ng y thay) ứng với chai c y loại 25cm + 5ml môi trường/ chai c y (5 ng y thay) ứng với chai c y loại 25cm 1.2.8 Môi trường nuôi c y [8] 1.2.8.1 Môi trường Thành phần môi trường nuôi cấy...
... cyclins (Clb5 and Clb6), and G2 cyclins (Clb1–4 Clb1–6) are all B-type cyclins (19) S pombe cyclins include Puc1 (a G1 cyclin), three B-type cyclins (Cig1 and Cig2; S-phase cyclins), and Cdc13 ... inappropriately from G1 (35) Not only does Rum1 bind Cdc2/Cdc13, it also targets Cdc13 for destruction, probably via the proteolytic machinery (42) 5.3 Patterns of Cyclin Expression in Yeast Two ... Murray, A W., and Kirschner, M W (1991) Cyclin is degraded by the ubiquitin pathway Nature 349, 132 138 Amon, A., Irniger, S & Nasmyth, K (1994) Closing the cell cycle circle in yeast: G2 cyclin...
... Wang or polyethylene glycol-polystyrene (PEG-PS) and peptide amide linker-polystyrene (PAL-PS) or Rink Amide 4methylbenzhydrylamine (MBHA) for carboxyl-free and amide peptides, respectively (Merck ... Macromolecular Crystallography Wiley, New York 55 McRee D (1999) Practical Protein Crystallography Academic Press, New York 56 Rhodes G (1993) Crystallography Made Crystal Clear Academic Press, New York ... University Press, New York 52 Carter C (2003) Methods in Enzymology Parts C and D Elsevier, New York 53 Drenth J (1999) Principles of Protein X-ray Crystallography Springer-Verlag, New York 54...
... soyasaponins DDMP refers to 2,3-dihydro-2, 5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP), glc , β-D-glucopyranosyl; gal, β-D-galactopyranosyl; glcUA, β-D-glucuronopyranosyl; ara, α-L-arabinopyranosyl; ... layer chromatography Topo I: DNA topoisomerase I Tr: trace below 0.1 μg/mL TS: Total soyasaponins UK: Unknown compound XAD-2: Amberlite Polymeric Adsorbent Xyl: β-D-xylopyranosyl xi SUMMARY Soyasaponins ... al 2003) Alkaline hydrolysis on the other hand, tends to produce partial hydrolysis of soyasaponins Partial alkaline hydrolysis has been reported useful for preparing non-acetylated group A soyasaponins...
... Detection Reagents, Amersham Pharmacia Biotech) Deglycosylation with N-glycanase and endoglycosidase H Deglycosylation with PNGase F and endoglycosidase H (NEB) was performed in a volume of 50 ... the rVRL-1-containing cell lysates with PNGase F; only the 80 kDa band could be observed Our data indicate that both high mannose-type glycosylation and complex glycosylation of rVRL-1 occurs These ... the glycosylation sites of rVRL-1 and mVRL-1 we used the PROSITE prediction tool (http://www.exp asy.org/tools/scanprosite; Gattika et al [13] ) for the detection of Asn-X-Ser/Thr Asn-glycosylation...
... aligned sequences will be shown in the display window for you to apply any analysis on them Usually at this stage you should first save your file in one of your favourite formats What we have just ... ready for you to apply any data analysis to them For example, you may click Phylogenetics|Distance methods|Nucleotide sequences, and then click the Done button in the ensuing dialog box A phylogenetic ... (fig 5) appears for you to type in the IP address of your server, your login ID and password Your password will not be displayed in the Password input field Any character you type into this field...
... Epithelial anlgwin(s) Brain ankyrin (Ank2) Brain ankyrin (Ank2) Epithelial ankyrin(s) Epithelial ankyrin(s) Lymphocyte ankyrin Erythroid ankyrin (Ankl) Brain ankyrin (Ank2) (1) Although cPLA2 ... Ca2+-independent PLA2 (16) PAF acetylhydrolases are structurally diverse isoenzymes that catalyze hydrolysis of the sn-2 aeyl group of choline glycerolipids containing an sn-1 alkyl ether linkage and a short-chain ... often hydrolyzed to generate intraeellular and intercellular messengers Phospholipase A-2(PLA2) enzymes catalyze hydrolysis ofsn-2 fatty acid substituents from glycerophospholipid substrates to yield...
... dual of the classical first order obstruction Already in this simple case standard methods of singular (co)homology theory yield only a necessary condition for MCC( f1 , f2 ) to vanish (cf [7, 2.2]) ... isomorphism—into deep problems of homotopy theory (compare the discussion in the introduction of [11]) Fortunately powerful methods are available in homotopy theory which imply, for example, that MCC( f , ... known as (a kind of) homotopy equalizer of f1 and f2 , plays a crucial role In general it has a very rich topology involving both M and the loop space of N Already the set π0 (E( f1 , f2 )) of...
... produced by B-cell type primary malignant lymphoma of the kidney Ann Hematol 1998, 76:221-224 Amezyane T, Lecoules S, Bordier L, Blade JS, Desrame J, Bechade D, Coutant G, Algayres JP: Humoral hypercalcemia ... laboratory signs of disease Figure shows an abdominal CT scan performed one year after surgery Discussion Immunohistochemistry immunopositivity for PTHrP clearly demonstrates that hypersecretion ... the tumor had led to hypercalcemia in this case Contrary to Adult T-cell leukemia/lymphoma (ATLL) in which hypercalcemia is common and almost always secondary to PTHrP hypersecretion, high serum...
... have addressed the issue of named-entity recognition in biology in different ways The NLP community has typically tried to identify names by analyzing the syntactic structure of sentences, making ... Res 2003, 13: 1231-1243 Kinase Pathway database [http://kinasedb.ontology.ims.u-tokyo.ac.jp] Muller H, Kenny E, Sternberg P: Textpresso: an ontology-based information retrieval and extraction system ... 67:49-61 Yapex [http://ellis.sics.se:8080/cgi-bin/Yapex/yapex.cgi] Tanabe L, Wilbur W: Tagging gene and protein names in biomedical text Bioinformatics 2002, 18:1124- 1132 AbGene [ftp://ftp.ncbi.nlm.nih.gov/pub/tanabe]...
... significant distance away from their family, they may not have anyone that would be willing to pick up and deliver the necessities they may need for daily living This was confirmed by the interviews ... SARS pandemic in 2003 (Blendon, 2006; Cava, Fay, Beanlands, McCay, & Wignall, 2005; CDC, 2003, 2003b, 2003c; Lo et al., 2005; National Advisory Committee on SARS and Public Health, 2003; Ooi, Lim, ... prescriptions they would need; 40% said they were very worried they might not get paid for the time when they were not at work and that they might lose their job or business; 33% said they were very worried...
... PCR, Polymerase Chain Reaction PDI, Protein Disulfide Isomerase PEG, Polyethylene Glycol PSS, Polystyrene Sulfonate PVP360, Polyvinyl Pyrrolidone 360 kDa SEM, Standard Error of Mean SG I, SYBR Green ... Sensitivity of the RT-PCR was improved by ~10-fold at an enhanced specificity of amplification Thermal stability and processivity of DNA polymerase enzymes were enhanced by crowding demonstrated by synthesis ... intensity against hydrodynamic radius A B Rh (nm) Rh (nm) Figure 13 A typical DLS readout of a macromolecular solution of Fc400 dissolving in HBSS showing a mean hydrodynamic radius (Rh) of ~13. 6...
... life cycle They are approximately 3.5, 2.4, 2.1, and 0.7 kb in length These transcripts are then polyadenylated at an identical polyadenylation site and transported to the cytoplasm, where they are ... delivery of therapeutic shRNA genes The viral vector delivery may achieve very high efficiency and long-term effect However, because of several associated safety concerns, systemic delivery of ... by colony formation assay (miRNA-106b-25 cluster)……………………………… 138 Figure 5.10 Anchorage-independent growth examined by colony formation assay (miRNA-182, miR-221, miR-222 and miR-224)…………… 139 ...
... synthesis in testis (Robic et al., 2008) The synthesis of androstenone (5 α-androst-16-en-3-one) from pregnenolone in testis is catalyzed by the enzymes cytochrome P450C17 (CYP17A1) and enzymes ... methods 22 (ChIP-chip or ChIP-seq) (Park, 2009) and chromatin structure can be determined by DNase I Hypersensitivity Site technologies such as DHS-Seq or DNase-Seq and DHS-chip (Boyle et al., ... 2012; Pryce et al., 2012) According to Pryce and Daetwyler (2012), the reliabilities of upto 60% in genetic gain is achievable in dairy cattle using genomic selection (Pryce and Daetwyler, 2012)...
... may be possible to protect the internal site(s) from digestion by incorporating 5-methyl-dCTP in place of dCTP during PCR If the restriction enzyme is sensitive to methylation of C then only ... does not generally occur when a thermostable proofreading DNA polymerase is used as these enzymes would remove any unpaired nucleotide they erroneously added Several commercial systems are available ... efficiency after prolonged storage Also, TA cloning only works efficiently with thermostable DNA polymerases, such as Taq DNA polymerase, which have terminal transferase activity However, for many PCR...
... fit in the 4-byte boundary That is why we not show the regular 4-byte boundaries for these addresses 21.8 Figure 21.5 Example 21.1, an ARP request and reply 21.9 Figure 21.6 Proxy ARP 21.10 Figure ... ARP reply is unicast 21.7 Example 21.1 A host with IP address 130 .23.43.20 and physical address B2:34:55:10:22:10 has a packet to send to another host with IP address 130 .23.43.25 and physical ... address and vice versa This can be done by using either static or dynamic mapping Topics discussed in this section: Mapping Logical to Physical Address Mapping Physical to Logical Address 21.2 Figure...
... in Northern hybridization for normalization of gcGLUT expression Phylogenetic analysis Phylogenetic analysis was performed by maximum parsimony using the PROTPARS program of the PHYLIP package ... kidney, while hypoxic induction was only observed in gill at h, and eye at and 170 h In vivo expression of 3.1-kb gcGLUT transcript was seemingly unaffected by both short and long-term hypoxia ... described by Zhou et al [14] The levels of dissolved oxygen were monitored continuously using a YSI Model 580 dissolved oxygen meter After the exposure period, fish were anaesthetized by immersion...
... polluted by human or animal faeces or urine Diseases in this category include cholera (Cholera vibrio), shigellosis (dysentery caused by Shigella spp.), typhoid (Samonella typhi), paratyphoid (Samonella ... which may cause diseases that vary in severity from mild gastroenteritis to severe and sometime fatal diarrhoea, dysentery, hepatitis or typhoid fever (WHO 1996) Most of them are widely distributed ... threatening “blue baby” syndrome, in very young children, which is a significant problem in parts of rural Eastern Europe (Yasso et al 2001) Some pesticides are applied directly on soil to kill...