11 re lanford d chavez fv chisari and c sureau 1993 lack of detection of negative strand hepatitis c virus rna in peripheral blood mononuclear cells and other extrahepatic tissues by the highly strand specific rtth reverse transcriptase pcr
báo cáo hóa học: " Vascular endothelial growth factor-A and chemokine ligand (CCL2) genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients" pot
... Additional file 1: Real Time Polymerase Chain reaction (PCR) Methodology of Real Time PCR; PCR cycling conditions and amplicon size of VEGF-A and CCL2; sequences and references of primers used ... emphasized that mean survival duration of reported ALS patients could not be ascertained Since elevated CCL2 initiates inflammatory reaction by increasing production of nitric oxide and other inflammatory ... and CCL2 in peripheral blood mononuclear cells (PBMCs) of these patients Subjects and methods 50 patients, born in North India and diagnosed with ALS were included from a convenience sample of...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
... (D) Dtco of rabbits injected with the supernatant fluids obtained from the indicated concentrations of PBMC with ngÆmL)1 of SEC1 (E) Dtco of rabbits injected with the supernatant fluids obtained ... SEC1 induces pyrogenicity via NADPH oxidase Fig The pyrogenic response in rabbits induced by the supernatant fluids of SEC1-treated human PBMC (A, B) Changes in the colonic temperature (Dtco) of ... the NF-jB to bind to DNA The NF-jB subunits were characterized by using a speci c antibody for the p50 or p65 subunit, and the results indicate that the NF-jB band intensity reduced in the presence...
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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc
... inhibition of SEBinduced arachidonic acid and MAPK activation in human lymphoid cells by 5-LO inhibitors such as curcumin, NDGA and MK881 (C Mendis, R Das, D Yang and M Jett, unpublished results) whereas ... importance of 5-LO as a pathway inter-connector in SEB-induced human PBMCs Similar results were observed in vascular smooth muscle cells, in which JNK-1 and MAPK were induced by arachidonic acid in a ... annealing temperature of 60 C and were subjected to PCR for 35 cycles in a thermocycler (PerkinElmer, Waltham, MA, USA) All sequences are indicated in the 5¢ to 3¢ direction and are abbreviated...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... Expression of ten putative MDSC-inducing factors was measured in MDSC-inducing (bold) and non-inducing HNSCC cell lines by qRT -PCR Increased CD33+ MDSC-induction capacity was associated with greater ... cell line-induced CD33+ and CD11b+ MDSC Arginase activity of CD11b+ and CD33+ MDSC subsets as measured by arginine degradation to urea and compared with normal myeloid cells Mean shown + SEM; data ... induction and MCF7 or NCI-H60 for CD11b+ MDSC induction B, Human CD33+ and CD11b+ MDSC are distinct subsets with a common HLA-DRlow Lineage- phenotype Phenotype of HNSCC cell line-induced CD33+ and...
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Báo cáo y học: "Immune complexes from rheumatoid arthritis synovial fluid induce FcγRIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-α by peripheral blood mononuclear cells" potx
... factor phages [9] and monocyte-derived dendritic cells [10] in ICinduced cytokine production in RA joints Earlier studies conducted by Jarvis and coworkers [31,32] demonstrated cytokine-inducing ... [14] In the present study, blocking of FcγRIIa resulted in markedly reduced IC-induced TNF-α production, indicating that the ICinduced cytokine production is at least partly mediated via FcγRIIa ... cytokine production induced by PEG precipitates TNF-α production induced by the precipitates was reduced by 55% with blocking of FcγRIIa, but no effect of blocking FcγRIII was apparent (Figure...
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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc
... peripheral blood mononuclear cells (PBMC) subpopulations: CD3/CD19, CD14, and CD16 PBMC were incubated with an antibody cocktail of CD3-Cy5, CD19-APC, CD16-FITC, and CD14-PE, then placed in the cell sorter ... implications Accumulated evidence suggests that CTC detected in the blood and DTC detected in the bone marrow of breast cancer patients are independent prognostic factors of disease free and overall ... method to detect CTC by identifying the source of the background signals and reducing them by the proposed method of negative immunoselection Our method was successful in reducing background CK19...
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Báo cáo y học: " Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis" pdf
... Identification of hRasGRP4 mRNA and protein isoforms in CD14+ myeloid cells Circulating in vivo-differentiated, unfractionated human PBMCs and PBMC-derived CD3+ T cells, CD14+ myeloid cells, and ... expression in peripheral blood A, Evaluation of hRasGRP4 mRNA levels in unfractionated peripheral blood mononuclear cells (PBMCs), and PBMC-derived CD14+ cells, CD14-/CD3-/CD19- cells, and T cells ... and end of the protein’s coding domain, the ~1.5-kb cDNA that encodes the normal isoform of hRasGRP4 was found to be abundant in the circulating CD14+ cells present in the PBMCs of normal individuals...
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Safe Blood Transfusion- Screening for Hepatitis B and Hepatitis C Virus Infections in Potential Blood Donors in Rural Southeast Asia
... packaged by core proteins in the golgi and secreted via exocytosis into the blood stream, where it can contact other liver cells and continue replication [24] In some cases, all HBV DNA can accumulate ... life and HBV DNA can be detected by nucleic acid amplification The presence of HBsAg and the absence of IgM anti-HBc also indicate chronic HBV infection The presence of HBeAg indicates high HBV DNA ... HCV antibody detection was introduced in 1989 and have resulted in the progress in early detection of HCV infection and contributed to the reduction of transfusion-related infections The first...
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Re examining the leader follower relationship and supportive leadership the role of the leader as attachment figure
... more of their direct reports to participate in the study Most supervisors had only two or three subordinates reporting directly to them, and we tried to include all of them in the study Subordinates ... that consideration and individualized consideration are distinct Individualized consideration focuses on the individual development of the follower Consideration is based on relations-oriented behaviors ... attitudes and behaviors could be addressed by considering the interaction between attachment avoidance and attachment anxiety The interaction of these two attachment dimensions can be used to predict...
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Anchorage and resistance to uprooting forces of eelgrass (Zostera marina L.) shoots planted in slag substrates
... affect the anchorage of the aquatic plants planted on them, which in turn, dictates the resistance of the plants to uprooting due to hydraulic forces (Handley and Davy, 2002) In their study, Handley ... significantly different from that of the pure ACS and 10% mixture, this inference cannot be conclusive Differences in the ACS and GS data could be attributed to differences between their properties ACS ... compactness of the substrate brought about by the combined effects of the high density of ACS and closeness of packing in DS However, since ACS data for the 20% silt-clay mixture were not significantly...
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Quantification of vitamin e and ç oryzanol components in RiceGermandBran
... corresponding standard The compounds were quantified by calibration curves of their structurerelated standards when their corresponding standards were not available Results are means ( standard deviation ... standard were created from seven concentrations using Microsoft Excel software The concentrations of individual components in the rice samples were determined using calibration curves of their corresponding ... tocopherols were calculated on the basis of the standard curves of their corresponding standards The standard curve of γ-tocopherol was also used to calculate γ-tocotrienol The UV absorption of...
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báo cáo hóa học:" Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cells" pptx
... 5'-GGGTGGCTTCGCCTACTTG-3' and reverse- 5'GACGCCCGTTTTCTTCTCAG-3'; ABCG1, probe FAMTCACACATCGGGATCGGTCTC and primers, forward 5'GTACTGACACACCTGCGAATCAC-3' and reverse- 5'TCGTTCCCAATCCCAAGGTA-3' The rat GAPDH ... blood cells did not occur until plasma concentrations moderately exceeded the 0.23 uM EC50 for ABCA1 induction in J774 cells Similarly, induction of ABCA1 and ABCG1 in peripheral blood cells by ... single dose (Figure 2C) and then diminishing as plasma drug levels decreased with clearance Finally, to determine whether the in vivo elevation of ABCA1 and ABCG1 mRNAs reflected the potency of...
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báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot
... broad specificity Reticulocalbin (RCN) RCN is a member of the EF-hand Ca(2+)-binding protein family and may regulate calciumdependent activities in the endoplasmatic reticulum lumen or post-ER compartment ... β-Catenin to Cadherins, that are involved in the formation and maintenance of the histo-architecture Plasmonogen activator inhibitor-2 (PAI-2) Involved in the regulation and inhibition of binding ... observation corresponds to investigations in breast cancer and small cell lung carcinoma In breast cancer a hypermethylation of the CpG island of the σ gene was found that leads to gene silencing and absence...
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Báo cáo sinh học: " Roles of adjuvant and route of vaccination in antibody response and protection engendered by a synthetic matrix protein 2-based influenza A virus vaccine in the mouse" pdf
... shortcomings of current vaccines could be lessened by a vaccine or vaccine adjunct that engendered protective Abs against viral structures of low or no variability, and thereby provided a constant ... four independent repeat vaccination and challenge experiments are compiled in Fig Figure Fine specificity of the M2e-MAP induced Ab response Fine specificity of the M2e-MAP induced Ab response ... 3B) The groups differed, however, with regards to resistance against descending infection The least resistance was seen in mice vaccinated with M2e-MAP and ODN by the s .c route and in fact did...
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báo cáo hóa học:" Aflatoxin levels, plasma vitamins A and E concentrations, and their association with HIV and hepatitis B virus infections in Ghanaians: a cross-sectional study" pdf
... were recruited from the community and all (clinic and community recruits) had no record of HIV positivity or symptoms of HIV infection (either from clinic records or self-report) HIVnegative individuals ... paper revision AK, JE and JW reviewed the protocol, interpreted data, and participated in the revisions of the paper YJ conducted lab analyses, interpreted lab data, and revised the paper CP supervised ... HBV infection was also a strong predictor of vitamin A deficiency Aflatoxin and HBV infection could have impacted the hepatic cells, thereby affecting vitamin A metabolism and storage The association...
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Influence of temperature on expander stability and on the cycle life of negative plates docx
... 80% of the useful capacity thereafter The capacity of the cells cycled at 25 8C is constant and very close to 80% of the useful capacity for about 280 cycles and begins to decrease thereafter The ... Vanisperse and Indulin on the performance of the batteries when cycled according to the ECE-15 EV test procedure 3.4 Cycle life tests of negative plates prepared with a mixture of Vanisperse and Indulin ... had a cycle life of 90 cycles, whereas those tested at high temperature (60 8C) endured 110 cycles before reaching their end -of- life The cells with 30% compression had a cycle life of 240 cycles...
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Báo cáo khoa học: "Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex" docx
... the virulence of the virus is much less than that of them [6, 13] Along with severe disease of leukocyte and lymphocyte counts, each number of MHC class II, CD1+, CD2+, CD4+, CD8+, CD4+CD8+ and ... first vaccination of modified live hog cholera virus In addition, significant decreases were observed in the proportions of MHC-class II, CD2+ and CD8+ lymphocytes at 3-weeks after booster injection ... and surface IgM+ B cells was decreased severely two days after inoculating virulent ALD strain of HCV, and each count of subpopulations was not recovered during the experiment period until death...
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Báo cáo y học: "Plasmatic B-Type Natriuretic Peptide and C-Reactive Protein in Hyperacute Stroke as Markers of Ct-Evidence of Brain Edema." pptx
... hypertensive cause in 12 Among ischemic strokes were cardioembolic, were lacunar, and the remainder (16) were ischemic non-cardioembolic (11 atherosclerotic and of undetermined cause) The location of stroke ... of the patients included in the study had overt evidence of infection on admission on the basis of standard blood and urine tests, X-film of the chest and temperature controls In table are presented ... values in hyperacute stroke might predict the development of CT-based evidence of delayed brain edema, and that these proteins increase in response to edema The second objective of the study is...
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Tìm thêm: xác định các nguyên tắc biên soạn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25