Đang tải... (xem toàn văn)
The objectives of the thesis: Study on the chemical compositions of three species: Allophylus livescens, Chirita halongesis and Oldenlandia pinifolia growing in Viet Nam to find bioactive compounds as the basis for further research.
MINISTRY OF EDUCATION AND TRAINING VIETNAM ACADEMY OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY KHIEU THI TAM STUDY ON CHEMICAL CONSTITUENTS AND CYTOTOXIC ACTIVITY OF SPECIES ALLOPHYLUS LIVESCENS GAGNEP, CHIRITA HALONGENSIS KIEW& T.H.NGUYEN AND OLDENLANDIA PINIFOLIA (WALL EX G.DON) KUNTZE] IN VIET NAM Major: Organic chemistry Code: 62.44.01.14 SUMMARY OF DOCTORAL THESIS HA NOI - 2018 This thesis is completed at: Vietnam Academy of Science and Technology Scientific instructors: Assoc Dr NGUYEN THI HOANG ANH Assoc Dr TRAN VAN LOC Thesis reviewer 1: Thesis reviewer 2: Thesis reviewer 3: The thesis will be presented to the scientific council at the Vietnam Academy of Science and Technology at , date , month ., year INTRODUCTION The urgency of the thesis Today, the economic development, the need for care and protection of the health of the community becomes more urgent Thus, the demand for medicine in preventing and treatment of incurable diseases especially cancer is increasing The plant resources in Viet Nam are rich and multiple with over 12000 species of 309 plant families Among them, 3900 species have been used for medicine However, the relationship between the chemical structure and pharmacological activity in many cases has been not clarified Allophylus livescens and Chirita halongesis, two endemic species of Ha Long Bay, have not yet studied on chemical compositions and biological activities Oldenlandia pinifolia is used in traditional medicine for treatment of inflammation, pain and other diseases and there has been only one report on the phytochemistry of this species To contribute to understanding, research and systematic evaluation of biologically active compounds from coastal plants and mangroves, and to propose directions for the exploitation and conservation of genetic resources of the rare plant species in Vietnam, we conducted research on the chemical constituents and cytotoxic activity of species: Allophylus livescens Gagnep., Chirita halongesis Kiew & T H Nguyen and Oldenlandia pinifolia (Wall ex G Don) Kuntze growing in Viet Nam The objectives of the thesis Study on the chemical compositions of three species: Allophylus livescens, Chirita halongesis and Oldenlandia pinifolia growing in Viet Nam to find bioactive compounds as the basis for further research The main contents of the thesis - Isolation of compounds from three species Allophylus livescens, Chirita halongesis and Oldenlandia pinifolia collected in Viet Nam by column chromatography - Determination of chemical structure of isolated compounds based on IR, MS, 1D-NMR, 2D-NMR spectroscopic analysis - Evaluation of cytotoxic activity of extracts and some isolated compounds The contribution of the thesis This is the first time the chemical constituents and cytotoxic activities of Allophylus livescens and Chirita halongenis species have been studied in Vietnam as well as in the world Two compounds were first isolated from Allophylus genus: 1,6,10,14-phytatetraen-3-ol and catechin Oleanolic acid, decaffeoylacetoside and β-hydroxy acteoside were found for the first time in Chirita genus This is the first study on the cytotoxic activity of Oldenlandia pinofolia species in Vietnam and in the world From this species, 14 compounds have been isolated and structurally elucidated, consisting of a novel compound (1,4,6-trihydroxy-2-methyl-anthraquinone) and two compounds (lutein afzelin) isolated for first time from Oldenlandia genus The n-butanol extract and four isolated compounds inhibited the proliferation of chronic myelogenous leukemia cells, induced apoptosis, and activated caspase (p