sciencedirect.com Phagocytosis and respiratory burst activity of haemocytes from the ivory snail, Babylonia areolata 2-3 phút Highlights • At 30 min, phagocytosis to Vibrio parahaemolyticus was higher than Saccharomyces cerevisiae • Phagocytic rate of granulocytes was significantly higher than that of hyalinocytes • Phagocytosis activity increased with time, after 90 min, no longer increased • Respiratory burst increased with time, after 120 min, no longer increased Abstract Haemocytes from the ivory snail, Babylonia areolata phagocytized Saccharomyces cerevisiae and Vibrio parahaemolyticus after 30 Haemocytes phagocytized V parahaemolyticus at a greater rate than they phagocytized S cerevisiae The phagocytic rate (PP) of V parahaemolyticus by granulocytes to was a little higher than that of S cerevisiae The phagocytic index (PI) of V parahaemolyticus by granulocytes was significantly higher than that of S cerevisiae The same was true of hyalinocytes The PP of granulocytes was significantly higher than that of hyalinocytes for each pathogen No difference in PI was observed in granulocytes and hyalinocytes Two defense mechanisms of B areolata were quantified using flow cytometry Haemocyte phagocytosis was quantified using fluorescent microbeads and respiratory burst activity was measured using H2O2 increases detected by 2′, 7′dichlorofluorescein diacetate Both phagocytosis and respiratory burst activity of the haemocytes increased over time After 90 the phagocytic rate no longer increased In the case of respiratory burst, the greatest increase in fluorescence occurred between 30 and 120 min, no further increase was seen after 120 These results showed unequivocally that a native (unstimulated) haemocyte oxidative burst was active in B areolata The aim of this study was to further the knowledge of immunology in gastropods   Previous  Next Keywords Babylonia areolata Haemocytes Phagocytosis Respiratory burst View full text Copyright © 2013 Elsevier Ltd All rights reserved  ... 7′dichlorofluorescein diacetate Both phagocytosis and respiratory burst activity of the haemocytes increased over time After 90 the phagocytic rate no longer increased In the case of respiratory burst, ... hyalinocytes Two defense mechanisms of B areolata were quantified using flow cytometry Haemocyte phagocytosis was quantified using fluorescent microbeads and respiratory burst activity was measured using... (PP) of V parahaemolyticus by granulocytes to was a little higher than that of S cerevisiae The phagocytic index (PI) of V parahaemolyticus by granulocytes was significantly higher than that of