This article was downloaded by: [Heriot-Watt University] On: 06 October 2014, At: 09:32 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Journal of Essential Oil Research Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tjeo20 Chemical Composition of the Leaf Oil of Evodia calophylla Guill from Vietnam a b Nguyên Anh Dũng , Trân Đình Thăng & Nguyên Xuân Dũng c a Faculty of Biology , Vinh University , 182, Le Duan, Vinh, Vietnam b Faculty of Chemistry , Vinh University , 182, Le Duan, Vinh, Vietnam c Faculty of Chemistry , College of Natural Science, Hanoi National University , 19-Le Thanh Tong, Hanoi, Vietnam Published online: 09 Dec 2011 To cite this article: Nguyên Anh Dũng , Trân Đình Thăng & Nguyên Xuân Dũng (2009) Chemical Composition of the Leaf Oil of Evodia calophylla Guill from Vietnam, Journal of Essential Oil Research, 21:1, 3-4, DOI: 10.1080/10412905.2009.9700092 To link to this article: http://dx.doi.org/10.1080/10412905.2009.9700092 PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content This article may be used for research, teaching, and private study purposes Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions E calophylla Chemical Composition of the Leaf Oil of Evodia calophylla Guill from Vietnam ˜ Anh Dung, Nguyˆen ˜ Faculty of Biology, Vinh University, 182- Le Duan, Vinh, Vietnam Tran ˆ Ðình Thang, ˘´ Faculty of Chemistry, Vinh University, 182- Le Duan, Vinh, Vietnam ˜ Xuân Dung,* Nguyˆen ˜ Downloaded by [Heriot-Watt University] at 09:32 06 October 2014 Faculty of Chemistry, College of Natural Science, Hanoi National University, 19-Le Thanh Tong, Hanoi, Vietnam Abstract The volatile components of the leaf oil of Evodia calophylla Guill from the Nghean province, Vietnam were analyzed by GC and GC/MS Thirty-three compounds, comprising 95.2% of the GC profile of the oil were identified The three major constituents were a-pinene (9.2%), (Z)-b-ocimene (17.5%) and (E)-b-ocimene (46.6%) Key Word Index Evodia calophylla, Rutaceae, essential oil composition, (Z)-b-ocimene, (E)-b-ocimene Introduction The genus Evodia belongs to the family of Rutaceae which covers 150 genera and perhaps 1500 species found in the warmer climate In Vietnam 10 species are found: E bodinieri Dode, E calophylla Guill., E crassifolia Merr., E lepta (Spreng.) Merr., E meliaefolia (Hance) Benth., E oreophila Guill., E pasteuriana A Chev ex Guill., E poilanei Guill., E simplicifolia Ridl., and E sutchuenensis Dode Evodia calophylla Guill is distributed in many places of the Central Vietnam: Binh Dinh (Quy Nhon), Khanh Hoa (Nha Trang, Ninh Hoa), Kon Tum (Dac Glay, Kon Plong), Gia Lai (Mang Yang), Dac Lac (Dac Nong), Lam Dong (Da Lat, Bao Loc) It has been traditionally used for wound healing, blood cleansing, as a sudorific and against rheumatism (1) In the course of the systematic study of Evodia species from Indochina, mono-, sesquiterpenes and other components of the leaf oil of E calophylla from Nghean province, Vietnam have been investigated Experimental Source: Evodia callophylla (Rutaceae), is a shrub tree that can grow up to 8–20 m high in Vietnam Leaves were collected in Pu Mat National Park, Nghean province A voucher specimen (DD7) has been deposited in the Herbarium of the Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology Fresh leaves were shredded and their oil was obtained by steam distillation for h at normal pressure, according to the Vietnamese Pharmacopoeia (2) The yield of the fresh leaf oil was 0.42% GC: About 15 mg of oil, which was dried with anhydrous sodium sulfate, was dissolved in mL of CH3OH (for spectroscopy or for chromatography) GC analysis was performed on a HP 6890 Plus Gas chromatograph equipped with a FID and fitted with 25 m x 0.25 mm x 0.25 mm film thickness HP-5MS column The analytical conditions were: carrier gas H2 with flow rate 35 cm/s, injector temperature (PTV) 250°C, detector temperature 260°C, temperature programmed 60°C (2 hold) to 220°C (10 hold) at 4°C/min The relative percentage amounts of the separated compounds were computed from FID data without the use of correction factors GC/MS: A Hewlett Packard 6890 Plus Chromatograph was fitted with a similar fused silica HP-5MS capillary column The conditions of use were the same as described above with He as carrier gas, and interface with a mass spectrometer HP 5972 MSD (70 eV) The temperature was programmed as reported Received: February 2005 *Address for correspondence Revised: December 2006 1041-2905/09/0001-03$14.00/0­—© 2009 Allured Business Media Vol 21, January/February 2009 Accepted: December 2006 Journal of Essential Oil Research/3 Dung ˜ et al Table I Percentage composition of the leaf oil of Evodia calophylla from Vietnam Compound RI Downloaded by [Heriot-Watt University] at 09:32 06 October 2014 a-pinene 939 camphene 953 sabinene 976 b-pinene 980 myrcene 990 a-phellandrene 1006 limonene 1032 1,8-cineole 1034 1042 (Z)-b-ocimene 1053 (E)-b-ocimene 2-nonanone 1092 linalool 1100 allo-ocimene* trans-piperitol 1207 citronellyl acetate 1356 a-copaene 1378 b-bourbonene 1385 b-elemene 1391 b-caryophyllene 1419 (Z)-b-farnesene 1444 a-humulene 1454 allo-aromadendrene 1460 germacrene D 1480 bicyclogermacrene 1494 germacrene A 1503 (E,E)-a-farnesene 1509 d-cadinene 1525 nerolidol* caryophyllene oxide 1581 isospathulenol 1628 a-muurolol 1645 patchouli alcohol 1659 farnesol* trace < _ 0.1%; * correct isomer not identified 4/Journal of Essential Oil Research % FID 9.2 trace trace 0.2 3.4 0.1 4.1 trace 17.5 46.6 0.1 0.4 0.3 0.3 trace 0.1 0.2 3.6 3.4 trace trace 0.1 0.4 2.0 0.3 0.2 trace 0.8 0.5 0.23 0.38 0.12 0.69 above Component identification was carried out by comparing MS data with those reported in the Wiley Library on Chemstation HP, and in some cases substances identified from oils known composition and also with standard substances (3–7) Results and Discussion With our best knowledge there are no previous reports on the chemical composition of the oil of E calophylla The compounds detected in the leaf oil of E calophylla from Vietnam are listed in Table I It can be seen that there were nearly equal amount of monoterpenes and sesquiterpenes The three major compounds of the oil were a-pinene (9.2 %), (Z)-b-ocimene (17.5%) and (E)-b-ocimene (46.6%) Other less prominant compounds were myrcene (3.4%), limonene (4.1%), b-elemene (3.6%), b-caryophyllene (3.4%) and bicyclogermacrene (2.0%) The other usually encountered monoterpenes and sesquiterpenes were present in amounts of from 0.1% to 0.8% Acknowledgments The authors wish to thank Vu Xuan Phuong, Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology for confirmation of the plant identity and Pu Mat National Park for help to collect samples References N.T Ban (Ed.), Checklist of Plant Species of Vietnam Agricultural Publishing House, Hanoi, Vietnam (2003) Vietnamese Pharmacopoeia Medical Publishing House, Hanoi, Vietnam (1997) S.R Heller and G.W.A Milne, EPA/NIH Mass Spectral Data Base U.S Government Printing Office, Washington, DC (1978,1980,1983) E Stenhagen, S Abrahamsson and F.W McLafferty, Registry of Mass Spectral Data Wiley, New York, NY (1974) A Swigar and R.M Siverstein, Monoterpenens Aldrich, Milwaukee, WI (1981) R.P Adams, Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectrometry Allured Publ Corp., Carol Stream, IL (2001) D Joulain and W.A Koenig, The Atlas of Spectral Data of Sesquiterpene Hydrocarbons E.B Verlag, Hamburg, Germany (1998) N.X Dung, P.H Ngoc, D.D Rang, N.T Nhan, N.Klinkby and P.A Leclercq, Chemical Composition of the Volatile Concentrate from the Flowers of Vietnamese Alstonia scholaris (L.) R., Br., Apocynaceae J Essent Oil Res., 13, 424–426 (2001) Vol 21, January/February 2009 ... With our best knowledge there are no previous reports on the chemical composition of the oil of E calophylla The compounds detected in the leaf oil of E calophylla from Vietnam are listed in Table... Natural Science, Hanoi National University, 19-Le Thanh Tong, Hanoi, Vietnam Abstract The volatile components of the leaf oil of Evodia calophylla Guill from the Nghean province, Vietnam were analyzed... 2006 Journal of Essential Oil Research/3 Dung ˜ et al Table I Percentage composition of the leaf oil of Evodia calophylla from Vietnam Compound RI Downloaded by [Heriot-Watt University] at 09:32