Ministry of Agriculture & Rural Development Collaboration for Agriculture & Rural Development Project 033/05 VIE Field evaluation and advanced vegetative mass-propagation technology for scaling up high-value plantations of Pinus caribaea and related hybrids in Vietnam Milestone 4: Output 1.2: Genetic Evaluation Trials Deliverables: Means of verification for payment • At least 5 genetic evaluation trials sites established to compare performance of locally available and introduced pines • Trial protocol documented and responsibilities for long term management and research evaluation analysis assigned Report Title: Commencement report for trials established in 2006 to compare performance of local and introduced tropical pines in Vietnam INTRODUCTION CARD Project 033/VIE seeks to review existing experience with and performance of several tropical conifer species in Vietnam, particularly Caribbean Pine, and to establish further trials of improved genetic material, including hybrids. The project will also provide capacity building for Vietnamese forest research institutions relating to Pinus tree improvement and mass propagation systems via training in Queensland and Vietnam, development of small-scale hedge and nursery infrastructure, and a study tour to Australia, involving innovative Vietnamese forest managers and researchers. Finally, the project will build awareness among large and small-scale forest growers including ethnic minorities, through the establishment of collaborative demonstration plantings in priority regions for Pinus plantation expansion, supported by appropriate training. This report relates to Output 1.2 of the Project: “Establish genetic evaluation trials to compare performance of locally available and introduced pines (especially Pinus caribaea and hybrids) on multiple sites throughout North Central, Central Coast and Central Highlands regions of Vietnam. PROGRESS Description of Sites Table 1 describes key features of six sites where genetic evaluation trials have been established. A seventh site near Dong Ha on the Central Coast is proposed for planting however arrangements have not yet been finalised. Photos for some of the sites are included in Appendix 3. Genetic Material Treatments Table 2 lists the range of genetic material treatments used at each site. Generally speaking, plants for the trials were raised at the RCFTI Nursery in Cam Quy, Ba Vi for sites in northern Vietnam and at the Cam Ly Nursery, Lam Dong Forest Research Centre for the Central Highlands sites. Additional local treatments were sourced from RCFTI (local Pinus caribaea var. hondurensis) and from local nurseries (Pinus merkussii). Experimental design and layout Generally speaking, the trials were laid out as 6 row x 6 tree (36 tree) plots for each treatment, arranged as a Randomised Complete Block (RCB) design with 4 replications. Appendix 1 includes a site map for each site. Early survival, early growth and re-filling Following early losses, some sites were re-filled with plants within the first couple of months of planting. In addition, some sites have recently been assessed for survival and early age height growth. Results will be reported at a later stage. Protocols for trial management, data collection, analysis and reporting The agency responsible for overall co-ordination of the trials is the Research Centre for Forest Tree Improvement (RCFTI) based in Hanoi. The nominated contact person is: Name: Mr Phi Hong Hai Position title: Deputy-director of RCFTI Contact Details: Phone No. 844 913309746/ 844 8389813 Email: phi.hong.hai@fsiv.org.vn Responsibilities for this position include: • Subject to availability of funding, co-ordinate trial assessments, based broadly on the schedule listed below: (a) Year 1: Survival, Height (cm) (b) Year 3: Survival, Height (cm), Diameter at breast height over bark (DBHOB) (c) Year 5: Survival, Height (dm), DBHOB (cm), Tree form & health assessment (d) Year 10: Survival, Height (dm), DBHOB (cm), Tree form & health assessment • Enter data into an electronic database for secure storage • Co-ordinate data analysis and reporting using existing resources. Australian partners may be able to assist. • Distribute results and reports to site managers and Australian Team Leader Appendix 2 lists the names of local site managers responsible for management of the relevant trial site. Responsibilities include: • Protect trials from damaging or competing agents such as over-topping weeds, animal browsing, fire and firewood collection; • Monitor health of trials and report any issues of interest (eg pest or insect damage, wildfire, general decline in tree health, interference by other forest users); and • Provide local assistance to assess trials as requested by RCFTI Co-ordinator FUTURE DIRECTIONS Future work required for this series of trials includes: • Plant remaining site at Dong Ha when weather conditions are suitable • Update this report to include Dong Ha details when confirmed • Collate and report early age growth and survival data for the various trials • Continue to maintain trial sites free from competing weeds and other interference • Periodic assessment and reporting as per responsibilities outlined in this document Table 1: Description of Pinus genetic evaluation sites planted in 2006 Site 1 Site 2 Site 3 Site 4 Site 5 Site 6 Province Ha Tay Vinh Phuc Vinh Phuc Lam Dong Gia Lai Quang Ninh Region North North North Central Highlands Central Highlands North Locality Cam Quy, Ba Vi Lap Thach Dai Lai Lang Hanh Pleiku Hoanh Bo Agency RCFTI Lap Thach District Forest Enterprise, part of Vinapaco Phu Ninh FRC Lam Dong FRC Gia Lai Tropical Forestry Centre Non-timber Forest Research Centre Latitude 21° 07’N 21° 29’ 46’’N 21° 20’ 36’’N 11° 38’ 15’’N 13° 59’N 20 o 54’ Longitude 105° 26’E 105° 27’ 5’’E 105° 43’50’’E 108° 15’53’’E 108° 00’E 106 o 50’ Elevation (m) 50 75 37-95 933 800 200 Annual Rainfall 1,680 1,387 1,500 1,730 2,270 2,061 Soil Poor soil, heavy clay at 50cm Reasonable, yellow medium clay Yellow medium clay + quartz outcrops Deep, red, well- drained Deep, red, well- drained Ferralit, medium to deep soil layer, red – yellow Date planted August 2006 29 July 2006 31 August 2006 7 July 2006 July 2006 31 August 2006 Cultivation Ripping to 70cm + 40cmx40cmx40cm manual pit cultivation 40cmx40cmx40cm manual pit cultivation Fertiliser mixed in 40cmx40cmx40cm manual pit cultivation 30cmx30cmx30cm manual pit cultivation Fertiliser mixed in 30cmx30cmx30cm manual pit cultivation Fertiliser mixed in 40cmx40cmx40cm manual pit cultivation Fertiliser mixed in Fertiliser 200gm superphosphate/tree 200gm superphosphate/tree 200gm superphosphate/tree 200gm superphosphate/tree 200gm superphosphate/tree 200gm superphosphate/tree Tree spacing 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) 3mx3m (1,111/ha) Table 2: Genetic treatments included in 2006 plant Pinus genetic evaluation trials: Number = treatment number for particular trial Site 1 Site 2 Site 3 Site 4 Site 5 Site 6 Province Ha Tay Vinh Phuc Vinh Phuc Lam Dong Gia Lai Quang Ninh Locality Ba Vi Lap Thach Dai Lai Lang Hanh Pleiku Hoanh Bo Pinus massoniana (PMASS) – Tam Dao 1 1 1 N/A N/A 1 P. caribaea var. caribaea (PCC) - Thua Thien Hue 2 2 2 2 2 12 P. caribaea var. hondurensis (PCH) – non select – Dai Lai 3 3 3 8 8 9 P. caribaea var. hondurensis (PCH) –select – elite seed from 11 plus trees, Dai Lai SPA 444338 P. caribaea var. hondurensis (PCH) – non select – Queensland 555446 P. caribaea var. hondurensis (PCH) –select – Queensland 6 6 N/A 5 5 7 P. caribaea var. hondurensis (PCH) –Cam Quy – non- select 7 P. caribaea var. hondurensis (PCH) – FRC source N/A 7 N/A N/A N/A N/A P. elliottii var. elliottii x PCH = PEE x PCH F2 hybrid - Queensland 887775 PCH x P. caribaea var. bahamensis (PCH x PCB) - Queensland 9 9 N/A N/A N/A 3 PCH x P. caribaea var. caribaea (PCH x PCC) – Queensland 10 10 8 6 6 2 PEE x PCH Series II clones (a) clone c3640, bare root cuttings (b) clone c 3136, bare root cuttings (c) clone c 4124, bare root cuttings 11 11a 11c 11b 11 11a 11b 11c N/A N/A N/A 10 10a 10b (11) Pinus merkussii (PMERK) N/A N/A N/A N/A N/A N/A P. caribaea var. bahamensis – Cam Quy 1 N/A 6 N/A N/A N/A P. caribaea var. bahamensis – Qld 4 P. kesiya (PKES) – non select - Dalat N/A N/A N/A 1 1 N/A P. kesiya (PKES) – select - Dalat N/A N/A N/A 9 9 N/A N/A = Not Applicable to this site Appendix 1: Site Design maps for CARD Pinus genetic evaluation trials established in 2006 Site Number Location Province Design attached 1 Cam Quy, Ba Vi Ha Tay Yes 2 Lap Thach Vinh Phuc See Excel file 3 Dai Lai Vinh Phuc Yes 4 Lang Hanh Lam Dong Yes 5 Pleiku Gia Lai Yes 6 Hoanh Bo Quang Ninh Yes 7 Dong Ha Quang Tri Not yet SITE 1: Experimental design for Pinus caribaea & hybrid pine at Cam Quy, Ba Vi – Ha Tay (Vietnam) (Part of CARD 033/05 VIE Project) + Date of planting: August 2006 + Trial design: RCB with 6 rows and 6 columns + Treatments: 11 + Replications: 4 + Trees/ plot: 36 + Planted spacing: 3 x 3 m + Hole: 40 x 40 x 40 cm + Fertilizer: 200 gram Super Phosphate/ plot + Total area: 2 ha(experimental area - 1.5 ha) DT1 Dai Lai DT2 (no select) QLQ (non select) 4:mass 10.c 15.c 34.c 11 6 5 1 2 9 cab 10 8 3 7 4 11 8 7 3 5 4 1 9 2 6 10 b c a 11 3 2 4 10 8 6 7 bac 9 5 1 11 1 5 9 a b c 7 2 6 10 4 8 3 Outside border, 1 row, 4 P.massoniana Acacia hybrid Note: Code Species Provernance 1 P.massoniana PMASS TamDao 2 P.caribaea var caribaea PCC ThuaThienHue 3 P.caribaea var hondurensis PCH Dai Lai (Not selected) 4 P.caribaea var hondurensis PCH Dai Lai (Selected) 5 P.caribaea var hondurensis PCH Queensland (Not selected) 6 P.caribaea var hondurensis PCH Queensland (Selected) 7 P.caribaea var hondurensis PCH CamQuy (Not selected) 8 P.elliottii x PCH F2 PEE x PCH Queensland 9 P.caribaea var hondurensis x P.caribaea var bahamensis PCH x PCB Queensland 10 P.caribaea var hondurensis x P.caribaea var caribaea PCH x PCC Queensland 11 P.elliottii xPCH F1 clone cutting PEE x PCH cutting Queensland 11a= C4124, 11b= C3640, 11c= C3136 SITE 2: Experimental design for Pinus caribaea and hybrid pine at Lap Thach – Vinh Phuc (Vietnam) 5555555555555555555555 5 5555555555555555555555555555555555555555555555555 xxxxxxaabbccxxxxxxxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 33333 x xxxx xa abbc cx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 33333 x xxxx xa abbc cx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 33333 x xxxx xa abbc cx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3333 x xxxx xa abbc cx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3333 xxxxxxaabbccxxxxxxxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 333 xxxxxxxxxxxxxxxxxxxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxbbccaa 333 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xb bcca a 33 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xb bcca a 33 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xb bcca a 33 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xb bcca a 33 xxxxxxxxxxxxxxxxxxxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxbbccaa 33 xxxxxxxxxxxxaaccbbxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 33 x xxxx xx xxxx xa accb bx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 33 x xxxx xx xxxx xa accb bx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 33 x xxxx xx xxxx xa accb bx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 x xxxx xx xxxx xa accb bx xx x x xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 1 xxxxxxxxxxxxaaccbbxxx x xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 3 1 xxxxxxxxxxxxxxxxxxxxx x xxxxxxxxccbbaaxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 3 11 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xc cbba ax xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 11 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xc cbba ax xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 11 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xc cbba ax xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 111 x xxxx xx xxxx xx xxxx xx xx x x xx xxxx xc cbba ax xxxx xx xxxx xx xxxx xx xxxx xx xxxx x 3 1111 xxxxxxxxxxxxxxxxxxxxx x xxxxxxxxccbbaaxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx 3 11111111111111111111111111 1 111111112222222222222222222222222222222222223 11111111111111111 222222222222222222222223 11111111111111111 222222222222222223 111111111111111 222222222 111111111 1111 1 2 3 4 5 6 7 8 9 101112131415161718192021 22 23242526272829303132333435363738394041424344454647484950515253545556575859606162636465666768 1 Pinus massoniana - PMASS X3mXXXXX 2 Pinus caribaea var caribaea - PCC ( Thõa Thiªn Hu Õ ) 3m 3 PCH (no select) Dai Lai XXXXXX 4 PCH (select) Dai Lai 5 PCH (no select) Queensland XXXXXX 6 PCH (select) Queensland 15m 7 PCH - FRC source XXXXXX 8PEE x PCH F2 (Queensland) 9 PCH x PCB (Queensland) C3640 XXXXXX 10 PCH x PCC (Queensland) 11 PEE x PCH cutting (Queensland) C3136 XXXXXX C4124 Experimental design for P inus caribaea and hybrid pine a t Ng o c My - La p Tha c h Fo re s try En te rpris e - P hu Th o Date planted: 29 July 2006 (As a part of CARD 033/05 VIE Project) North dire ction (b) ( c ) Xuan Hoa vil. 12 km Highway 2C 4 km 15m Road (a) Rep. I Rep. II Rep. III Rep. IV 6 11 8 7 51 1024 3 9 111 79 63105 2 8 4 95 11 8 2 1 6310 725869310 1 7 4 411 Terrace Field of Cassava SITE 3: Experimental design for Pinus caribaea and hybrid pine at Dai Lai – Vinh Phuc (Vietnam) (Part of CARD 033/05 VIE Project) + Date of planted: 31 August 2006 + Design as randomized complete block with 2 rows and 4 columns + Treatments: 8 + Replications: 4 + Trees/ plot: 36 + Total area: 1.02 ha (with experimental area: 0.54 ha) 7 3 1 8 6 2 5 4 7 6 5 8 1 3 4 2 7 4 3 5 2 8 6 7 1 4 6 2 5 3 8 1 Rep. 4 ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ Rep. 2 Rep. 1 1 edge row P . mass TDao 5 rows (4) P C C -Hue 4 rows (3) P . mass- TDao 1 edge row P . mass TDao 1 edge row P . mass TDao 1 edge row P . mass TDao 1 edge row P . mass TDao 1 edge row P . mass TDao PCC-Hue ( 4 ) PCH (No select) Dai Lai ( 1 ) P. mass T D ao ( 3 ) PCH (no select ) Treatments: In genetic trial 1. P. massoniana Tam Dao 2. PCC – Hue 3. PCH (no select) Dai Lai 4. PCH (select) Dai Lai 5. PCH (no select) QLD 6. PCB Cam Quy (Ha Tay) 7. PEE x PCH (F2) QLD 8. PCH x PCC (F1) QLD (1 8 is tree code in layout) On buffer zone 1. PCH (no select) Dai Lai 2. PCH (no select) QLD 3. P.Mass Tam Dao 4. PCC Hue (Note: plot 4 & 5 of Rep.4 destroyed by illegal harvest by local people and re-planted with A. mangium) Rep. 3 SITE 4: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE AT LANG HANH – LAM DONG (Part of CARD 033/05 VIE Project) (Date planted: July 2006) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 36 trees/plot 1 2 3 5m 8 5 6 2 4 1 4 5 6 7 4 9 3 7 5 7 8 9 3 2 1 8 6 9 Rep. 1 Rep. 2 Rep. 3 Road among plots 5m 3 6 1 7 8 9 5 4 2 Rep. 4 Road 5m Treatments: 1. P.kesiya (no select) Da Lat (PKES) 2. P.caribaea var caribaea – Hue (PCC) 3. P.caribaea var hondurensis (select) Dai Lai 4. P.caribaea var hondurensis (no select) Queensland 5. P.caribaea var hondurensis (select) Queensland 6. P.caribaea var hondurensis x P.caribaea var caribaea – Queensland (PCH x PCC) 7. P.elliottii var. elliottii x PCH F 2 Queensland (PEE x PCH) 8. P.caribaea var hondurensis (no select) Dai Lai (PCH) 9. P.kesiya (select) Da Lat [...]... Dai Lai (No-select) 10 PEE x PCH (F1)-clonal cuttings includes C3640: 60 cuttings; C4124: 54 cuttings (1 10 is code that shown in above experimental trial) Appendix 2: Details of local Site Managers responsible for CARD Pinus genetic evaluation trials established in 2006 Site Number 1 Location Province Local Site Manager Cam Quy, Ba Vi Ha Tay Mr Nguyen Dinh Hai rcfti@vnn.vn 2 Lap Thach Vinh Phuc Mr... Experimental design for Pinus caribaea and hybrid pine at Hoanh Bo – Quang Ninh (Vietnam) (Part of CARD 033/05 VIE Project) + Date planted: 24 July 2006 + Design as randomized complete block with 2 rows and 5 columns + Treatments: 10 + Replications: 4 + Trees/ plot: 36 + Total area: 2 ha (with experimental area: 1.35 ha) + Planted spacing: 3x3m + Hole: 40 x 40 x 40 cm + Fertilizer: 200 gram Super Phosphate/... P.kesiya (no select) Da Lat (PKES) 2 P .caribaea var caribaea – Hue (PCC) 3 P .caribaea var hondurensis (select) Dai Lai 4 P .caribaea var hondurensis (no select) Queensland 5 P .caribaea var hondurensis (select) Queensland 6 P .caribaea var hondurensis x P .caribaea var caribaea – Queensland (PCH x PCC) 7 P.elliottii var elliottii x PCH F2 Queensland (PEE x PCH) 8 P .caribaea var hondurensis (no select) Dai... Tay Mr Nguyen Dinh Hai rcfti@vnn.vn 2 Lap Thach Vinh Phuc Mr Luc Nhu Trung Trung-frc@yahoo.com.vn 3 Dai Lai Vinh Phuc Mr Dinh Van Quang 4 Lang Hanh Lam Dong Mr Hua Vinh Tung 5 Pleiku Gia Lai Mr Nguyen Van Cuong 6 Hoanh Bo Quang Ninh Mr Nguyen Huy Son Appendix 3: Photos of CARD Pinus genetic evaluation trials established in 2006 (Top Left: Site 1, Cam Quy, Ba Vi; Top Right: Site 2, Lap Thach; Centre:...SITE 5: EXPERIMENTAL DESIGN FOR PINUS CARIBAEA AND HYBRID PINE AT GIA LAI - PLEIKU (Part of CARD 033/05 VIE Project) (Date of planted: July 2006) P.kesiya trial planted 1986 1 7 13 19 25 31 2 8 14 20 26 32 3 9 15 21 27 33 4 10 16 22 28 34 5 11 17 23 29 35 6 12 18 24 30 36 36... Macadamia 7 6 PCH DL No -select 9 5 PCH Qld No-select PCC Hue Rep 1 ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ ♣ 3 8 6 7 1 10 9 5 4 2 3 8 10 5 Rep 2 4 2 P mas TD Top of hill 6 1 9 7 Botanic Garden Road P massoniana Tam Dao Treatments: 1 P massoniana Tam Dao 2 PCH x PCC Queensland 3 PCH x PCB Queensland 4 PCB Queensland vµ PCC Hue 5 PEE x PCH (F2) 6 PCH Qld (No-select) 7 PCH Qld . technology for scaling up high-value plantations of Pinus caribaea and related hybrids in Vietnam Milestone 4: Output 1.2: Genetic Evaluation Trials Deliverables: Means of verification for. Ministry of Agriculture & Rural Development Collaboration for Agriculture & Rural Development Project 033/05 VIE Field evaluation and advanced vegetative mass-propagation technology. building for Vietnamese forest research institutions relating to Pinus tree improvement and mass propagation systems via training in Queensland and Vietnam, development of small-scale hedge and