1 2010 iWln!IIKl»IWIMWMU!W^ li|lV<*>ii||il)ji>M'^Wi;)My mnG BAI HOC: ¥ iHn^IBll 9 I' '%& ii SZS»»«i«4M,Wrf«SE5».llMa»ilS»Yit^ BOYTE BAG CAO KET QUA NGHIEN ClTU DE TAI CAP BQ Ten de tai: XAC DEVH MAM BENH KY SINH TRUNG GAY BENH CHO NGU'CM TRONG RAU VA THUY SAN DUOC NUOI TRONG TlT NGUON NUdC THAI TAI MQT SO THANH PHO VA NONG THON MIEN BAC HOI DONG NGHIEM THU CAP BO Chu tich Hoi dong: Uy vien nhan xet 1: TS. Tran Nhu Du(mg GS.TS. Phan Thi Kim Uy vien nhan xet 2: TS. Nguyen Mgnh Hiing Ha Noi-4/2010 BOYTE BAO CAO KET QUA NGHIEN CtTU DE TAX CAP BO rri A J. A . > • Ten de tai: XAC DINH MAM BENH KY SINH TRUNG GAY BENH CHO NGlTCXl TRONG RAU VA THUY SAN Dirac NUOI TRONG TlT NGUON NUdC THAI TAI MOT SO THANH PHO VA NONG THON MIEN BAC Chu nhiem de tai: PGS.TS. Nguyen Van De Ca quan chu tri de tai: Trncmg Dai hoc Y Ha Noi Cap <^uan ly: Bo Y te Ma so de tai (neu co): Thai gian thuc hien: tu thang 01 nam 2008 d%n thang 12 nam 2009 T6ng kinh phi thuc hien de tai 1.400 trieu dong Trong do: kinh phi S>iKH 1.400 trieu ddng Nguon khac (neu c6) trieu dong Ha Noi 4/2010 BOYTE CONG HOA XA HOI CHU NGHIA VIET NAM Dpc lap Tir do Hanh phiic So: /iHIJ /QD-BYT Hd Npi, ngdy o2^ thdng U ndm 2008 QUYET DINH Ve viec phe duyet nhiem vu bao ve moi trucrng trong nganh y te BO TRUONG BO Y TE Can cu Nghi dinh so 188/2007/ND-CP ngay 27/12/2007 cua CMnh phii quy dinh chuc nang, nhiem vu, quyen han va co ca'u t6 chiic Bo Y te; Can cii Bien ban hpp ngay 27/3/2008 cua Hoi d6ng tham dinh nhiem vu bao ve moi trudng duoc thanh lap theo Quyet dinh so 667/QD-BYT ngay 27/02/2008 cua Bo trudng Bo Y te; Theo de nghi cua Cue truang Cue Y te du phong va Moi truang, QUYET DINH: Dieu 1. Phe duyet nhiem vu "Xac dinh mam benh ky sinh triing gay benh cho ngucfi trong rau va thuy san duac nuoi trong tir nguon nude thai tai mot sd thanh phd va ndng thdn Mien Bac" - Dan vi chu tri thue hien: Trudng Dai hoe Y Ha Ndi. - Thdi gian thue hien: 2008-2009. - Kinh phi thuc hien: 1.400.000.000d (Mdt ty bdn tram trieu dong chan) trr nguon su nghiep mdi trudng. Dieu 2. Thu truang dan vi chu tri thuc hien nhiem vu cd trach nhiem: ehi dao, trien khai thuc hien cac ndi dung nhiem vu bao ve mdi trudng theo de cuang da duae Hpi dong tham dinh thdng qua (de cuang chi tiet kem theo); thuc hien diing eac quy dinh hien hanh cua nha nude ve hoat ddng bao ve mdi trudng va chi tieu tai ehinh; dinh ky bao cao ket qua thuc hien ve Cue Y te du phdng va Mdi trudng de tong hap bao cao Lanh dao Bd. Dieu 3. Quyet dinh nay cd hieu luc ke tii ngay ky ban hanh. Dieu 4. Cac Ong, Ba: Cue trudng Cue Y te du phdng va Mdi trudng, Vu trudng Vu Ke' hoach Tai chinh, Thu trudng dan vi chu tri thuc hien nhiem vu va cae dan vi lien quan chiu trach nhiem thi hanh Quye't dinh nay./. Noi nhan: ^„ ===^iyB6 TRU6NG Nhir Digu 4; J^-i ^p^'^.^ , ^^T.,^ BT. Nguyin Quoc Trieu (de b/c); / /."^^fi?^*^^^^^ Liru:VT,DPMT f /W^^ ""^Tf mh Quan Huan Phu luc 4 Danh sach tac gia cua de tai/nhiem vu KH&CN cap Bo (Danh sach nhung ca nhan da dong gdp sang tao chil ylu cho de tai dupe sap xep theo thii tu da thoa thuan) 1. Ten De tai/nhiem vu: Xdc dfnh mam b^nh ky sinh triing gdy b$nh cho ngu&i trong rau vd thuy sdn du^c nuoi trong tir nguon nu&c thdi tgi mpt sd thdnh pho va nong thon mien Bdc • Md so: 2. Thuoc ChuoTig trinh (neu c6): 3. Thdi gian th\rc hien: Tu thang 01/2008 den 12/2009 4. Ca quan chu tri: Trudng Dai hpc Y Ha Npi 5. Bp chu quan: Bp Y te/Cuc Y te Du phdng va Mdi trudng Viet Nam 6. Danh sach nhiing ngudi tham gia (tac gia): TT 8 10 11 HQC h^m, hgc vj, ho va ten PGS.TS Nguyen Van De ThS. Phan Thi Huang Lien PGS.TS Le Thanh Hda GS.TS. Phung Dac Cam CN. Le Thanh Phuang ThS. Truong Thi Kim Phuang KTVY. Do Duong Thang TS. Pham Ngpc Minh CN. Pham VSn Khiem CN. Nguyen Thj Hau CN. Cao Van Huyen Chitky ^Jj^u^j/lU/^fZ/t ~^S^ Thu truOTig c(y quan chu tri de tai/nhiem vu jfTOr^u' ky vaiddng d^u) BO Y TE CONG HOA XA HQI CHU NGHIA VIET NAM TRUONG DAI HOC Y HA NOI Dpc lap - Tu do - Hanh phiic Ha Npi, ngay 30 thang 12 nam 2009 GIAY XAC NHAN CHI TIEU TAI CHINH DE TAI NCKH Ten de tai: Xdc dinh mdm benh ky sinh triing gdy benh cho ngu&i trong rau vd thuy sdn duoc nudi trdng tit ngudn nu&c thdi tgi mpt so thdnh pho vd nong thdn miin Bac Chii nhiem dh tai: Nguyin Van Be Co quan chii tri dl tai: Trudng Dai hoc Y Ha Npi Co quan quan ly dh tai: Bh tai c4p bp Y tl Thdi gian thuc hien dl tai theo quylt dinh phe duyet ciia BYT: 01/2008-12/2009 Tdng kinh phi duac duyet: 1.400.000.000 ddng Tdng kinh phi da cSp: 1.400.000.000 ddng Tdng kinh phi da quylt toan: f 4o\). o rO . l^X) \ -y Cac hoat ddng chi tieu ciia de tai dam bao diing theo de cuang da duyet va dam bao qui dinh ve tai chinh ciia Nha nude. F •> \ F Sd kinh phi cdn lai: (chi de khoang 20 - 25 trieu ddng) phuc vu cho nghiem thu cap CO sd va nghiem thu c4p BYT: ^ XAC NHAN CUA PHONG TAI CHINH TRUONG DAI HOC Y HA NOI \£lS' \ Y^ CHU NHIEM DE TAI PGS.TS Nguyin Van De BAO CAO KET QUA NGHIEN ClfU BE TAI/NHIEM VU CAP BQ Chii nhiem dl tai: Nguyen Van De Co quan chii tri de tai: Trirotig Dai hoc Y Ha Noi Cap quan ly: Bo Yte F -y F Ma sd de tai (neu cd): 1. Ten de tai: Xdc dinh mdm benh ky sinh triing gdy b$nh cho ngu&i trong rau vd thuy sdn duoc nudi trdng tie ngudn nu&c thdi tgi mpt sd thdnh pho vd ndng thdn miin Bdc 2. Chii nhiem dl tai: PGS.TS. Nguyin Van DI 3. Co quan chii tri dl tai: Trudng Dai hpc Y Ha Ndi 4. Co quan quan ly dd tai: Bp Y te 5. Thu ky dl tai: ThS. Phan Thi Huong Lien 6. Phd chii nhiem de tai hoac ban chii nhiem de tai (neu cd): 7. Danh sach nhiJng ngudi thuc hien chinh: - Nguyin Van DI Trudng Dai hoc Y Ha Ndi - Phan Thi Huong Lidn Trudng Dai hpc Y Ha Ndi - Le Thanh Hda Vien Cdng nghe sinh hpc - Phung Die Cam Vien Ve sinh Dich te Trung uong Tmong Thi Kim Phuang Trudng Dai hpc Y Ha Ndi - Tap thi Bd mdn Ky sinh tning, Trudng Dai hpc Y Ha Ndi 8. Cac dl tai nhanh (dl muc) ciia de tai (neu cd) (a) DI tai nhanh 1 (de muc 1) Tdn de tai nhanh: - Chu nhiem de tai nhanh: (b) Dl tai nhanh 2 - Ten dl tai nhanh Chii nhiem dl tai nhanh 9. Thdi gian thuc hien dh tai tu thang 01 nam 2008 din thang 12 nam 2009 r e Nhung chCr viet tat ADN ATSLa BM cs C. sinensis D KST Hp Ht M NCKH L PCR S. erinacei SLRN SLGN TP T WHO Acide Deoxyribonucleic Au trung san la Bdmdn Cdng su (cdng tac vidn) Clonorchis sinensis Tning giun dGa Ky sinh tning Haplorchis pumilio Haplorchis taichui Trung/Su trung giun mdc Nghien cuu khoa hpc Au tning giun luan Angiostrongylus Polymerase Chain Reaction Spirometra erinacei San la mdt nhd San la gan nhd Thanh phd Tning giun tdc World Health Organization Muc luc Noi dung Trang Bao cao kdt qua nghien ciiu de tai cap Bd 3 Nhirng chu vidt tat 4 Cac san pham dat duac ciia de tai/du an 6 Ban tu danh gia 7 Chuang 1: Dat v4n de 10 Muc tieu 13 Chuang 2: Tdng quan tai lieu 14 F Chuong 3: Ddi tuang va phuang phap 22 Chuang 4: Kdt qua nghien cuu 28 Tai thanh phd 28 Tai ndng thdn 31 Chuang 5: Ban luan 37 Chuang 6: Kit luan 43 Khuyin nghi 46 Tai lieu tham khao 47 Phu luc: Mdt sd hinh anh sinh dia canh va Ky sinh triing trong nghien cuu 49 CAC SAN PHAM DAT DlTQC CUA DE TAI/NHIEM VU 1. Bang ket qua sd lieu nghien cuu (xem kit qua bao cao kem theo) 2. Bao cao ket qua xac dinh loai ciia mam benh ky sinh tning trong thiiy san va rau tuoi sdng sir dung nude thai (xem ket qua bdo cao kem theo) 3. Cac bao cao khoa hpc duac dang tai: Cd 3 bai bao khoa hpc dang tren cac tap chi nhu sau: - Nguyin Van DI, Phan Thi Huang lien, Truang Thi Kim Phuang, Pham Ngpc Minh va cs. O nhiem m4m benh tren ca nudi bSng nude thai tai thanh phd va ndng thdn tinh Nam Dinh. Tgp chi Thdng tin YDupc. Sd 8/ 2009. Tr. 19-21 Nguyin Van De, Phan Thi Huong lien, Truang Thi Kim Phuang, Pham Ngpc Minh, Le Thanh Phuang, Pham Van Khiem, Nguyen Thi Hau. Danh gia d nhidm mam benh giun san ciia mdt sd loai thuy san duac nudi trong ao bang nude thai sinh hoat. Tgp chi YDuac hoc Qudn su. Sd 9/ 2009. Tr.29-32 - Le Thanh Phuang, Nguyen Van Dd, Pham Ngpc Minh, Phan Thi Huong lien, Truang Thi Kim Phugng va cs. Mam benh ky sinh tning tren rau duac tudi bang nude thai tai thanh phd va ndng thdn tinh Nam Dinh. Tgp chi YDiepc hpc Qudn su. Sd 9/ 2009. Tr.29-32 4. Cac khda luan tdt nghiep Y khoa vd van de lien quan den nhiem vu. Hai khoa luan tdt nghiep Cii nhan Y hpc (Le Thi Thanh Loan va Dinh Cdng Tru) bao ve nam 2009 (cd ban phd td bia kem theo) va se cd 2 khda luan tdt nghiep bao ve nam 2010. 5. DI xuat cac giai phap phii hgp. r ^ F Giai phap cho ngudi san xuat: De san xuat thiiy san va rau dam bao an toan thuc phim la khdng su dung nude thai chua duac xu ly diing quy trinh d ca ndng thdn va thanh phd. - Giai phap cho ngudi tieu dung: Hien nay, viec sijr dung nude thai dk nudi trdng thiiy san va tudi rau cdn phd bidn nen ngudi tieu dung khdng nen an thuy san sdng va rau sdng (chua duac nau chin). [...]... tai BM Ky sinh tning, Dai hgc Y Ha ndi 7 \ Tham dinh lodi bang sinh hgc phan tii tai Vidn Cdng nghe sinh hgc 2 Doi tirong nghien cun: r \ - Cdc loai thuy sdn bao gdm cd chep, cd trdm, cd me, cd rd phi vd cd trdi, luon, F F tdm, cua, ech, dc •\ ^ •* 7 - Cdc lodi rau bao gdm rau mudng, rau can, rau ngd, rau cai soong, rau edi, rau r diep - Cdc mlu nude, bun tai ao/hd nghien ciiu - Cac mau ky sinh trung... Angiostrongylus spp F > ^ Nhan xet: Trdn tat cd cdc loai rau tai TP Nam Dinh va Tp Hd Ndi ddu cd mam benh giun sdn, rieng TP Hda Binh chi cd 3 loai rau cd mam benh giun san Sy khac nhau giua cdc dilm cd y nghia thdng ke vdi rau dilp vd rau mudng, p' sinh triing (giun, san va don bao) trong san pham phuc vv con ngudi (rau, thuy san) dupe nudi trong bang nude thai tai mpt sd thanh phd va ndng thdn mign Bic - Djnh loai cac mam benh k)'' sinh tning nay bang hinh thai hpc va sinh hoc phan tir - Dg xuat mdt sd giai phap nham bao vg ngudi sir diing mpt so thue pham cd nguy C nhigm ky sinh trimg O Kgt qua thvc hien la: a) Xgt nghigm cho 2.700... mdu cho vdo dung dich tidu co, thudng de trong lp 100ml chiia 50ml dung dich tidu co gdm: HCl 8 ml + 6 g pepsin + nude cdt vua dii 1.000 ml Trdn ky va dl tii Im 37°C trong 4 gid Cho thdm 50 ml nude vd lac ldn Lpc todn bd mdu bdng ludi cd kich thudc Id 1 x 1 mm vd nia bdng nude mudi sinh ly 0,85% F y F Lang can khodng 7-8 lan ddn khi can trong F Quan sat au trung dudi kinh liip khi soi can dyng trong. .. petri vd thu thap F y au trung bang pipet pasmer Thu au tning cho ien lam kinh de dinh loai bdng hinh thdi hpc F F Bao quan au tning trong nude mudi sinh ly 0,85% Bao quan lau dai trong formalin 10% dd dinh loai bang hinh thdi hpc \ 7 \ Bdo qudn lau ddi trong cdn 70% de dinh loai bdng sinh hpc phan tii 5.2 Ky thugt xet nghiim tim au triing sdn Id trong oc: - Md dc lay gan-tuy ddt ldn lam kinh, day lamen . ylu cho de tai dupe sap xep theo thii tu da thoa thuan) 1. Ten De tai/nhiem vu: Xdc dfnh mam b^nh ky sinh triing gdy b$nh cho ngu&i trong rau vd thuy sdn du^c nuoi trong . A J. A . > • Ten de tai: XAC DINH MAM BENH KY SINH TRUNG GAY BENH CHO NGlTCXl TRONG RAU VA THUY SAN Dirac NUOI TRONG TlT NGUON NUdC THAI TAI MOT SO THANH PHO VA NONG. QUYET DINH: Dieu 1. Phe duyet nhiem vu "Xac dinh mam benh ky sinh triing gay benh cho ngucfi trong rau va thuy san duac nuoi trong tir nguon nude thai tai mot sd thanh phd va ndng thdn