Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... S¯adhan¯ a Vol. 35, Part 4, August 2010, pp. 461–468. © Indian Academy of Sciences Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source SKJAIN∗,PANAIKandPRHANNURKARRaja ... field. the magnetic field can cause diffusion of the plasma particles to the wall of the plasma cham-ber. The measured magnetic field profiles along the axial distance for (a) mirror magnetic field, and ... than 5 G). Imperfections in466 S K Jain, P A Naik and P R HannurkarFigure 4. The measured magnetic field profiles along the axial distance for (a) mirror magnetic field, and (b) flat magnetic field. the...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... species was detected with g values at 2.031,1.994, and 1.951. The resonance started to develop atpotentials ‡ 0 mV and was stable at potentials up to +350 mV. The loss and formation of the resonance ... of AF499 was identified as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence()35 to )23, AAANNNTTATATA) ... with an apparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the polypeptides of the purified enzyme. N-Terminal sequen...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... bands from SDS ⁄PAGE was performed as described by Bamford et al. [22].MSMALDI-TOF MS was performed using an Ultraflex TOF ⁄TOF instrument (Bruker Daltonik, Bremen, Germany). Pro-teins and ... minimal med-ium [19] with 2% lactose as a carbon source, 1% potassiumphthalate as a buffering agent (pH 5.5), 0.1 mm CuSO4 and 1% tyrosine as an indicator substrate was used. The trans-formants ... CoomassieBlue staining.Kinetic parameters, pH optimum and stability and thermal inactivationThe Km and kcatvalues for T. reesei TYR2 using l-dopa,p-coumaric acid and p-tyrosol as substrates...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... concentrationsless than 25 mM. However, N-acetyl derivatives, namelyN-acetylglucosamine (GlcNAc), N-acetylgalactosamine(GalNAc) and N-acetyl mannosamine (ManNAc) werebetter inhibitors than their ... animal glycoconjugate. They actas an important component of the ligands recognized by thelectins. Recognition can be affected by specific structuralvariations and modifications of sialic acids and ... Horse and rabbit erythrocytes, which have a highcontentof4-O-Ac-NeuAc and 9-O-Ac-NeuAc, respectively,showed the highest titers with the lectin. Human A and O,sheep and goat that contain largely...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... intermediate of Fig. 5. Putative genomic organization of the human phosphohistidine phosphatase gene. AstandardBLASTsearch, with AF164795 as the query, wasperformed against the nonredundant databases ... PROCEDURESMaterialsPhosphoamidate was prepared according to the method of Wei and Matthews [4]. Suc-Ala-His-Pro-Phe-pNA waspurchased from Bachem AG, Switzerland. Malachite greenreagent was obtained ... tracedby its absorbance at 315 nm, appeared as a peak at 0.33MMDEA and was essentially stable in this buffer at +4 °C for at least 4 months. The phosphate content of thephosphopeptide was analysed...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... amount of Bip-Pro in the extracellular uptake mediumwas quantified according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and a ... inves-tigation because of their physiological importance and their pharmaceutical relevance as drug carriers [1–6].Both transporters catalyse the uptake of most dipep-tides and tripeptides and a variety ... (Fig. 1A) and the synthe-sis strategy (Fig. 1B) of Bip-Pro. The purity of thecompound was assessed by TLC, analytical RP-HPLC, MS and NMR, and was found to exceed98%. As expected for an Xaa-Pro...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... SmRXR1 can interact with mammalian coac-tivators of transcription, and S. mansoni coactivators of transcription may have a similar mechanism to SmNR1 ⁄ SmRXR1. Recently four NR coactivators,SmGCN5, ... 13 and 23 contain nonspecific competitor. Lanes 4, 14 and 24 contain DR0 ascompetitor. Lanes 5, 15 and 25 contain DR1 as competitor. Lanes 6, 16 and 26 contain DR2 as competitor. Lanes 7, 17 and ... (GenBankAF129816) were previously ligated into activation domainvector pACT2 to form pACT-SmRXR1 and pACT-SmRXR2, and ligated into DNA binding domain vectorpAS2 to form pAS-SmRXR1(CF) and pAS-SmRXR2...
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Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles doc
... treated with dithiothreitol (5 mm) to reducethe disulfides, and assayed as above. Thiols were quanti-fied by comparison of peak areas to standard curves of those standards. Coenzyme A was measured ... theratio of protein concentration to flavin concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a significant level of identity to putative NADH oxidases ... for the CoADR reaction.Thermostability and thermoactivity of the CoADRphCoADR is stable for months at both )80 °C and )20 °C, and has half-lives of > 100 and 39 h at 85° and 95 °C, respectively....
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc
... Storz, G. &Rhee, S.G. (1994) Cloning and sequencing of thiol-speci®cantioxidant from m ammalian brain: alkyl reductases and thiol-speci®c antioxidant de®ne a large family o f antioxidant ... membrane was exposed to X-ray ® lm at )20 °Cor,when using an intensifying s creen, at )80 °C.RNA for Northern analyses was isolated as describedabove and separated in a 1.3% agarose/formaldehyde ... and 72 °Cfor2min).Inaddition to the above degenerated reverse primer twodirect primers 5¢-AACCATGGCCTCCCACGCCGAGAAGCC(G/C)CTG-3¢ and 5¢-AACCATGGCCAGCCACÓ FEBS 2002 Peroxiredoxin from Chlamydomonas...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... microorga-nisms, plants and animals and their function and physio-logical roles have been investigated and identified [12,13].N-acyl-D-amidohydrolase (EC.3.5.1.81, N-D-AAase) is an enzyme capable ... SDS/PAGE and Western blotting of N-D-AAase. (A) SDS/PAGE (10% acrylamide). M, Molecular mass standards; lane 1, crudeextract; lane 2, heat treatment of crude extract; lane 3, protein afterDEAE-Toyopearl ... upstream of the N-D-AAase gene is:5¢…AGGACAGACGAATG…3¢ (The Shine-Dalgarnosequence and start codon are in bold). The recombinantplasmid was named pTrc 2A- damA3 without His-tag.Expression and...
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