... eliminate, or take advantage of partition effects that are sure to bepresent.HPLC: A Practical User’s Guide, Second Edition, by Marvin C. McMasterCopyright © 2007 by John Wiley & Sons, Inc.8.1 ... specific use separationsAFFINITY CHROMATOGRAPHY 101APPENDICES 201APPENDIX A Personal Separations Guide 203APPENDIX B FAQs for HPLC Systems and Columns 205APPENDIX C Tables of Solvents and Volatile ... gradient controller. Because of this, isocratic systems are simpler andHPLC: A Practical User’s Guide, Second Edition, by Marvin C. McMasterCopyright © 2007 by John Wiley & Sons, Inc.less...