... with O-benzotriazole-N,N,N¢,N¢-tetramethyluronium hexafluorophosphate inDMF, and a fourfold excess of the Fmoc-protected aminoacid was used to maximize yield of the N-hydroxybenzo-triazole-amino ... report the chemical synthesis, bio-chemical production, and solution structure of preCbnB2, and compare it with the structure of the maturebacteriocin, CbnB2.Materials and methodsChemical synthesis ... following condi-tions were used: elevated temperatures of up to 50 °C,O-7-azabenzotriazole-1-yl-N,N,N¢,N¢-tetramethyluroniumhexafluorophosphate as activation agent, and N-methyl-pyrrolidinone...