... Vuntil the protein samples were within the stacking gel, and then at a constant current of 18 mA (500 V) for 5–6 h. The proteins were transblotted onto a poly(vinylidene difluoride)membrane and probed ... forcytochrome c [4, 5]. The remaining 10 subunits of the mammalian enzyme, namely, IV, Vb, VIa, VIb, VIc, VIIa,VIIb and VIII are encoded by the nuclear genome,synthesized in the cytosol and imported ... cellular ATP (nmolÆmg protein)1) 10.3 ± 1.132 7. 04 ± 0.7 74 22 ± 1.986 11 ± 1 .43 1Respiration coupled ATP synthesis in isolatedmitochondria (nmolÆmg protein)1)36 ± 3. 24 28 ± 5.7 84 ± 9.52 37 ± 5.12Enzyme...