... Masasuke YoshidaChemical Resources Laboratory, Tokyo Institute of Technology, JapanF1-ATPase i s inactivated by entrapment of MgADP in catalytic sites and reactivated by MgATP or Pi. Here, using ... it was 30±40 Uámg)1,whichare in the normal range for thermophilic F1-ATPase activity.ATP hydrolysis assayATP hydrolysis was measured using an ATP-regeneratingsystem as a decrease in A 340 of ... ondrial F1-ATPase and inhibitscatalysis is bound at a catalytic site. Biochim. Biophys. Acta 1020,43±48.11. Muneyuki, E., Makino, M., Kamata, H., K agawa, Y ., Y oshida,M. & Hirata, H....