... vitro [66], and disassembly of active unitsfrom an oligomeric (storage) state of a-crystallin wasproposed, upon structural analysis of aA-crystallin by site-directed spin labelling, as a model ... containing clones were s elected by blue/whitescreening using the LacZ system, propagated in LB broth, and examined by restriction analysis for plasmids incorpo-rating inserts of t he appropriate size, ... thermotolerance in E. coli and thusmay be necessary for chaperoning in vitro.Oligomerization in the context of thermotolerance, as described in this s tudy, is not as thoroughly investigated as the association...