... 2MGdnHCl), showing increase in t he fluorescence intensity,indicating temporary exposure of the hydrophobic patches of the protein during unfolding (Fig. 3B). The p ossibility of the occurrence of the ... lM)andANS(50lM), in different concentra-tions of GdnHCl (1–5M).Determination of the lectin activitySugar binding activity of the samples was measured by theenhancement in the intrinsic flu orescence of ... highspecificity f or methyl a-D-galactopyranoside (Me a-gal).The folding pathways of oligomeric proteins involve bothintramolecular and intermolecular interactions. The dena-turation of pea and peanut...