... proteins detected by Westernblotting are shown in (A) and (B). Silverstainingisshownin(C)and(D)inbluesuperimposed with the spots of oxidized pro-teins shown in red. Protein labels: 1, a-tubulin;2, ... of the side chains of amino acids within a protein. In particular, the side chains of cysteine, histidine, methionine,tryptophan and tyrosine are susceptible to ROS-inducedmodifications [2,11,12]. ... silver-stained protein map, we inferthat a-tubulin and b-tubulin are targets of PDT-inducedprotein oxidation. H2O2treatment resulted in labeling ofendoplasmic reticulum proteins. Under...