... reportedpreviously for both rat and human enzymes [5,32–35].In conclusion, we analyzed the contribution of the N- and C-terminal regions of GCPII to its enzymatic properties and structure/folding. The ... sequences from human GCPII, human transferrin receptor, yeast aminopeptidase Y,S. griseus aminopeptidase, and Caenorhabditis elegansmGCP fragment, His377, Asp387, Glu425, Asp453 and His553 were ... 1. Schematic diagram of the human glutamate carboxypeptidase II(GCPII) domain structure and GCPII variants used in this study. Thefigure shows wild-type human GCPII and its truncated or taggedvariants....