... sustained activation of ERK1 ⁄ by NGF, but not the transient activation of ERK, could effectively induce miR221 and 222 in PC12 cells Finally we identified the BH3-only protein Bim, which is involved ... involved in NGF- dependent neuronal survival [17–19], as a potential target of miR-221 and 222 Results NGF stimulation induces miR-221 /222 in PC12 cells Treatment of PC12 cells with NGF for 48 h induced ... miR221 and 222 derive from the same pri-miRNA [21] Following NGF stimulation, the expression level of Fig NGF induces expression of the microRNAs miR-221 and 222 in PC12 cells (A) NGF- induced...