... normal- or reversed-phase high-performance liquid chromatography (HPLC) (8) or anion-exchange chromatography, e.g., high-performance anion-exchange chromatography (HPAEC) on two CarboPac PA-100 ... Dowex 50X-12 H+-form resin Sephadex GM25 2.4 Hydrazinolysis 0.5-mL screw-capped V-bottomed Reacti-vials™ (Pierce and Warriner, Chester, UK) Anhydrous hydrazine (Pierce and Warriner) Whatman CF-11 ... butan-2,3-diol (two times the molar excess over periodate) for 40 at 0°C in the dark 3.3 Release of Core Disaccharide (Galβ 1-3 GalNAcα–) from Mucin Using Endo-α-N-Acetylgalactosaminidase (O-Glycanase)...