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Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus

Báo cáo y học:

Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps

... stimulation by distinct inflammatory injuries [3,44] EPC have been shown to display a higher proliferative potential [45] and may migrate to regions of the circulatory system with injured endothelia, including ... hypothesis whether the homing of intravenously administered bone marrow- derived EPC occurred in damaged lung tissue after the setting of severe tissue injury, as previously shown in part in an abstract ... http://respiratory-research.com/content/8/1/50 Figure Rat bone marrow- derived EPC in culture Rat bone marrow- derived EPC in culture A highly purified population of EPC was isolated from hindlimb bone marrow...
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Proteomic analysis of airway inflammation in murine asthma models

Proteomic analysis of airway inflammation in murine asthma models

... protein components of the epithelial lining fluid of the airways and alveoli Distinct change in certain protein levels in BAL fluid has been correlated to allergic airway inflammation Many investigations ... staining for fibronectin 113 27 Representative 2-D gels in chronic asthma model 120 28 Protein grouping using Gene Ontology Annotation 124 29 Interactions between actin binding proteins and actin ... RT-PCR analysis in acute asthma model 96 20 Serum level of OVA-specific IgE in chronic asthma model 106 x 21 Airway responsiveness in chronic asthma model 107 22 H&E staining for airway inflammation...
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A multiplex comparative proteomic analysis of hypoxia influence in the presence and absence of p53 in HCT116 cells

A multiplex comparative proteomic analysis of hypoxia influence in the presence and absence of p53 in HCT116 cells

... Other kinases, such as casein kinase I and II, protein kinase A, 38 CDK7, DNA-activated protein kinase, and Jun-NH2 kinase (JNK), also mediate phosphorylation of p53 and play important roles in ... that in turn “protects” radiation-damaged DNA from restoring to an undamaged state Stable DNA damage accumulates and leads to an increased lethality from a given dose of radiation in cells, inducing ... criteria 4.3 Top funcitons and diseases identified by IPA 76 4.4 Tabulation of common proteins regulated in cells under 81 hypoxia in the presence and absence of p53 as well as in the absence of p53...
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Báo cáo y học:

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

... intravenously injected into nude mice and the distribution and migration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous implantation of allogeneic MSCs in the ... transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis in necrotic femoral heads, ... intravenously implanted allogeneic MSCs could directionally migrate to femoral heads, and survive especially in the necrotic femoral heads, the mechanisms underlying the directional migration of MSCs...
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Báo cáo y học:

Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"

... molecules in the course of hypoxia-induced apoptotic pathways Such analysis of trophoblastic cells will, in the near future, yield insights into the mechanisms of preeclampsia and other hypoxia-related ... and by modulating phosphorylation of JNK In conclusion, various pro- and antiapoptosis-related proteins were expressed in the process of hypoxia-induced apoptosis in the trophoblastic cell line ... gene, but by modulating phosphorylation of JNKs Thus, Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad Bag-1 may also enhance apoptosis by inhibiting the expression of Bcl-2...
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Báo cáo y học:

Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"

... immunoglobulin chains were also found to be significantly increased in aged thymocytes Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte fate ... the increased levels of many of these genes identified in the array analysis Moreover, Western blot analysis of thymocyte lysates also confirmed that IgM increases with age (Figure 3) Flow cytometry ... antibody-producing cells in the thymus, even with advancing age Western blot analysis of IgM quantities demonstrated levels of IgM heavy chain in the thymocytes of 24-month-old mice as high as in...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... overexpressing a- synuclein A proteomic analysis is expected to identify cellular alterations that are associated with dopamine treatment and modulated by a- synuclein overexpression, without any a priori ... eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative differences, regardless of whether or not a- synuclein ... above (Tables S2 and S3) In both cases, bioinformatic analysis revealed that the NF-jB pathway could be involved in determining the effects of dopamine treatment and a- synuclein overexpression Accordingly,...
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báo cáo hóa học:

báo cáo hóa học:" High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia" pot

... represented in the AML samples Intra-individual analysis of the spot patterns showed a high correlation between the sample from peripheral blood and bone marrow (Table 2) On/off-phenomena of the identified ... Expression of the plasminogen activator inhibitor-2 (PAI2) was only found in patients E and F with the subtyp FAB M0 and M4, respectively This finding is inline with data from the PAI-2 serum levels of ... investigations of the proteome using 2D-electrophoresis Furthermore, protein expression profiling is likely to further impact the analysis of mechanisms involved in acute leukemia by examining routinely...
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báo cáo hóa học:

báo cáo hóa học:" A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice" docx

... of the sterility testing in compliance with eu Pharmacopoeia 2.6.1 (sterility) and the validation of the potency assay in an ATMP that is constituted of bone- marrow mononucleated cells used in ... using cell systems and in vivo assays using animal models As concerning the use of bone marrow mononucleated cells in cardiac repair, the importance of characterizing the functionality of injected ... is the validation of a commercial system (Charles River Endosafe PTS) for the determination of bacterial endotoxins in compliance with Eu Pharmacopoeia 2.6.14 (bacterial endotoxins), the validation...
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Báo cáo y học:

Báo cáo y học: "Dietary fatty acid intake affects the risk of developing bone marrow lesions in healthy middle-aged adults without clinical knee osteoarthritis: a prospective cohort study" docx

... an increased risk of developing BMLs after adjusting for energy intake (Table 2, model 1) For each SD increase in dietary intake of saturated fatty acids, the risk of developing BMLs over years ... important in maintaining joint integrity and the development of OA, this study suggests that dietary modification of fatty acid intake may be one strategy in the prevention of knee OA which warrants ... the dietary intake of fatty acids was measured in a valid fashion [22], this was based on a single measure of nutrient intakes 10 years earlier Although significant underreporting of fat intake...
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s Membrane proteomic analysis of pancreatic cancer cells docx

s Membrane proteomic analysis of pancreatic cancer cells docx

... overexpressed in BxPC-3 cells whereas phosphoglycerate kinase was over-expressed in AsPC-1 cells, which agrees to the results obtained by the proteomic approach Discussion Metastasis is a highly ... lymphangiogenesis and lymphatic metastasis in PDAC cells [29] The described proteomic approach is reproducible for analysis of membrane proteins in cultured pancreatic cancer cells We observed consistent SDS-PAGE ... chemiluminescence detection (Applied Biological Materials) Results The purpose of this study was to demonstrate a membrane proteomic analysis of PDAC cells and to identify differentially expressed membrane...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx

Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx

... Dou KF, Yang GS, Willerson JT, Geng YJ: Combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells increases benefits in infarcted swine hearts Arterioscler Thromb Vasc Biol ... neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation Journal of Biomedical Science 2010 17:75 Submit ... decreased muscle cell apoptosis and improved blood reperfusion of ischemic muscle following combined therapy of highdose simvastatin and bone marrow-derived MSCs Bone marrow-derived MSCs have been...
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Báo cáo y học:

Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx

... enrichment of Lin- PCs in the pneumonia lungs Few of these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the lung) , therefore approximately 14.6% of the initially ... shown in the BM homing study by Szilvassy and colleagues [38]), increasing the number of infused progenitor cells will increase the number of these cells that home to injured lung tissues and become ... hours) in the lung while the Lin- PCs were preferentially retained (24 hours) there during pneumococcal pneumonia Furthermore, these progenitor cells also remained in the lung tissues and rarely migrated...
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Báo cáo y học:

Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt

... Expression of genes in the BMP2+ cells associated with plasticity, and mesodermal and NCSC phenotypes BMP2+ cells are still in a state of plasticity BMP2+ cells significantly upregulate Oct4 and Nanog ... 50 µm 20 µm Analysis neural crest stem cell associated transcripts in BMP2+ cells Figure of Analysis of neural crest stem cell associated transcripts in BMP2+ cells (a) RT-PCR analysis for the ... 20 µm Analysis adipocyte and macrophage associated markers in BMP2+ cells Figure of Analysis of adipocyte and macrophage associated markers in BMP2+ cells (a) RT-PCR analysis of adipocyte specific...
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Báo cáo y học:

Báo cáo y học: "Large-scale and high-confidence proteomic analysis of human seminal plasma" pptx

... study and two proteomics datasets published recently [10,11] Catalytic 305 Figure GoMiner analysis of the molecular function of identified proteins GoMiner analysis of the molecular function of ... can be estimated very roughly by the number of peptides identifying each protein (or more accurately by the number of peptides observed divided by the number of theoretically observable peptides ... proteins High-confidence and high-coverage analysis of the seminal fluid proteome Genome Biology 2006, 7:R40 information Our analysis found the proteins classically known to be present in seminal...
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