Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain
Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain
... studies of the structures and functions of the EEN and its three domains Native PAGE, Circular Dichroism and NMR were used to exploit the interaction of Ca2+ with EEN and its domains 2-D NMR and ITC ... acids sequence of ΔBAR domain of EEN Green letters stands for the SH3 domain; Black letters stands for the random coil domain The ΔBar domain which consists of the SH3 domain and the random coil ... cell and purified with affinity and gel filtration columns The interactions of Ca2+ and a peptide carrying the proline rich domain (PRD) with EEN and its three domains were investigated with...
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The mechanism of action of sprouty2 characterization of the interaction between sprouty2 and PKCdelta
... the mechanism of action by Spry2 121 3.3.7 Increasing levels of PKD1 enhances the interaction between PKCδ and Spry2 122 3.3.8 Verification that PKD1 increases the interaction between Spry2 and ... 89 3.1.5 3.2 Characterizing the interaction between Spry2 and its interacting partners Discussion and Conclusions 89 Characterization of interaction between Spry2 and PKCδ 91 3.2.1 Spry2 interacts ... PKCδ-KD prevents the interaction between Spry2 and endogenous PKCδ 130 3.3.13 Increasing PKCδ-KD levels decrease the endogenous PKCδ-Spry2 interaction 132 3.3.14 Interaction between PKCδ and PKD1 is...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
... months time [10 2] Acute cortical damage to auditory processing structures might be assessed objectively in a complementary manner, via studies of the MMN [10 3] 15 2 10 11 12 13 14 15 16 17 18 Closing ... Shappell SA, Brandt ME Psychophysiology of N200/ N400: A review and classification scheme Advances in Psychophysiology 19 91; 4:43 -10 6 Hoffman JE Event-related potentials and automatic and controlled ... stimulus, and omitted-stimulus paradigms Brain Topography 19 98; 11 :14 1 -15 1 67 Polich J, Hoffman D P300 and handedness Psychophysiology 19 88; 35:497-507 68 Hopper KD, Patel S, Cann TS, et al The relationship...
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Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... knowledge, the work described here and in the related publications [1,7] is the first kinetic analysis of the in vitro interactions Cyt f plastocyanin and plastocyanin Fig Comparison of ionic strength ... primitive cyanobacteria at the time of chloroplast origin had poorly defined interactions between Cyt f and plastocyanin and/ or plastocyanin and photosystem I, and subsequently the nature of the ... plastocyanin reacts with Cyt f, involving a relatively large interface [3], it is positive [41] We can conclude that the transition state for binding in the reaction between plastocyanin and photosystem...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
... for recombinant and native GAPDH with a multit using a common value of Km and different values of kcat The estimated parameters had a value of 25 lM for the Km, and the kcat for recombinant and ... those of native GAPDH The decrease of the catalytic constant is a fast process compared to the decrease of the K0.5 for BPGA These changes are most likely linked to conformational changes in the ... (Eqn in the main text) The sigmoid shape of the curve is detailed in the inset ant)] A multit was also performed The common value of Km was 143 15 lM and the kcat for recombinant and native GAPDH...
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The interaction between climate and the processing of coated papers in printing and finishing pot
... 18 Climate and Paper The interaction between climate and the processing of coated papers in printing and finishing Table of contents V Circumstances within the printer’s power to control I Introduction ... The situation in paper production and printing industry ˿ The situation in printing and finishing industry ˿ Circumstances in the workshop ˿ Paper handling 11 ˿ ˿ ˿ ˿ Blistering Breaking in the ... situation in printing and finishing industry Normally speaking, there are few climate related problems in printing and finishing As a result, climate only becomes an issue when printing problems,...
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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx
... this minireview is to summarize what is currently known about the molecular mechanisms of osmotic stress resistance, osmosensing and signal transduction in C elegans Osmosensing and signaling in ... process of interest, allows genes to be ordered into pathways, and can provide important and novel mechanistic insights into the molecular structure and function of proteins In addition to forward genetic ... factor signaling in the hypodermis regulates whole animal fluid balance [10] The intestine of adult C elegans is comprised of 20 epithelial cells that function in digestion and nutrient absorption In...
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Mechanical characterization of biopolymer and the effects of mechanical properties of extracellular matrix on cell behaviours
... MECHANICAL CHARACTERIZATION OF BIOPOLYMER AND THE EFFECTS OF MECHANICAL PROPERTIES OF EXTRACELLULAR MATRIX ON CELL BEHAVIORS WONG LONG HUI (B.Eng.(Hons.), NTU) A THESIS SUBMITTED FOR THE ... in cell and extracellular matrix interactions The first part of the thesis involved the use of conventional macrorheological technique to uncover the rich mechanical properties of collagen, the ... the cancer cells and the physical microenvironment, through cell- cell and cell- ECM interactions, determines the progression of the invasion process, as shown in Figure 1.4 [39] The interactions...
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Functional characterization of HGF and its receptor c met in zebrafish development
... paracrine signaling in liver development The coexpression of hgfa and c- met in pectoral fin, hgfb and c- met in proneprhic duct also indicate their paracrine signaling in the development of these ... Characterizing HGF and its receptor c- met s role in zebrafish development (Manuscript in preparation) v LIST OF FIGURES Fig.1.1 Schematic representation of proHGF/SF, HGF/ SF and the c- Met receptor ... X CHAPTER INTRODUCTION .1 1.1 Discovery of HGF and its receptor c- met 1.1.1 Discovery of HGF 1.1.2 Discovery of c- met and identification of c- met as the receptor...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... impaired phosphorylation of Tyr394 did not appear to influence the binding of tau to Fyn- SH2, as there was no significant difference in the amount of Y394F 2930 Fig Tau interactions with Fyn- SH3 are not ... phosphorylation mediates the association of tau with Fyn- SH2, but not with Fyn- SH3 This demonstrates that different molecular mechanisms exist for these two distinct interactions of Fyn and tau, with probably ... status of tau also affects the binding of tau to Fyn- SH3, CHO cells were cotransfected with Fyn together with either wild-type or the YF mutant forms of tau Cell lysates containing equal amounts of...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
... desolvation of the phosphate group Nevertheless, although the binding enthalpy of the inhibitors is negative, the binding enthalpy of 6PG is Table Binding parameters of substrate and substrate ... A binding isotherm and the fitted data for the binding of aPyADP to the enzyme are shown in Fig The binding stoichiometry was close to two Table Binding parameters of coenzymes to 6PGDH from Trypanosoma ... these analogues have high affinity and to help in rational drug design, we undertook a thermodynamic characterization of substrate and analogue binding to T brucei 6PGDH, in both FEBS Journal...
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Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf
... Divergences of rat alkaline phosphatase isozymes T Harada et al A B Fig Stereoviews of the residues near metal-binding site in (A) rat intestinal alkaline phosphatase- I (rIAP-I) and (B) rIAP-II ... Residues indirectly associating with the metal via water molecules Fig Immunological detection of rat intestinal alkaline phosphatase (rIAP) isozymes in rat small intestine Western blotting was ... alkaline phosphatases Evidence that intestinal alkaline phosphatase is a late evolutionary development Gastroenterology 91, 277–286 11 Xie Q & Alpers DH (2000) The two isozymes of rat intestinal alkaline...
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Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx
... the identification and characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans We show that these enzymes are heterotetramers, like the decaprenyl diphosphate synthase ... synthases in mice and humans need two proteins (i.e both mSPS1 and mDLP1 or both hDPS1 and hDLP1, respectively) to be active The success of reconstitution of solanesyl and decaprenyl diphosphate synthases ... whereas the side chain in mice and C elegans has nine units and that in S pombe and humans has ten isoprene units [17] One type of ubiquinone is dominant in each organism but a minor type(s) is also...
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hydrothermal synthesis and characterization of - feooh and fe2o3 uniform nanocrystallines
... agglomerate and form fusiform nanocrystallines Finally, the ␣ -FeOOH Fig The EDS spectra of the as-prepared porous fusiform ␣-Fe2 O3 nanocrystallines 220 X Liu et al / Journal of Alloys and Compounds ... pattern of the rodlike ␣ -FeOOH nanocrystallines taken on a mass of ␣ -FeOOH nanorods reveals the satisfactory crystallinity of the sample, which can be indexed to the pure Fig TEM image of the ... electron microscope images of a typical sample of fusiform ␣-Fe2 O3 nanocrystallines obtained at 140 ◦ C for 24 h and indicate the large quantity and good uniformity ␣-Fe2 O3 nanocrystallines were...
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preperation and characterization of sno2 and wox - sno2
... 40 and 90 nm (see Fig 2b and c) SN, SN-650 and SN-850 powders showed speci®c surface areas of 31, 31 and 17 m2 gÀ1, respectively W 1-6 50 and W 5-6 50 pellets showed speci®c surface area of 25 and ... representing the line of absorbance zero in Fig 5a, a broad, weak, and negative 93 Fig Time resolved effects of mbar of NO2 at RT on FT-IR spectra of (a) SN-650, (b) W 1-6 50 and (c) W 5-6 50 pellets Spectra ... Effects of mbar of the NO2/O2 mixture at 1508C on FT-IR spectra of SN-650 and W 5-6 50 pellets (curves and 2, respectively) The dotted line represents the zero of absorbance 3.3 Electrical characterization...
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