Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro
Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro
... ISOLATION AND MOLECULAR CHARACTERIZATION OF GENES ASSOCIATED WITH SHOOT REGENERATION OF MUSTARD (BRASSICA JUNCEA) IN VITRO GONG HAIBIAO (M.Sc SJTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... of genes associated with shoot regeneration from leaf disc explants of mustard (Brassica juncea) in vitro Discussion 84 The cloning of a phi class glutathione S-transferase gene and effects of ... identify and characterize genes associated with shoot regeneration of mustard in vitro These were achieved by the isolation of 87 cDNAs, with 56 homologous to known genes or ESTs, using mRNA...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... precipitate fraction; sup, supernatant fraction The molecular weight standard (lane M) is indicated on the right A novel high-activity D-hydantoinase from Jannaschia sp CCS1 Fig Purification of HYDBp ... high-activity D-hydantoinase from Jannaschia sp CCS1 Y Cai et al Experimental procedures Genome mining and identification of putative D-hydantoinase genes Using the amino acid sequence of HYDBp (AAL37185)...
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báo cáo khoa học: " Isolation and functional characterization of cold-regulated promoters, by digitally identifying peach fruit cold-induced genes from a large EST dataset" pptx
... gAgTTggATgggTCCTCTgC CCAAACCAAAgCCAgTTTCATTCA CCAggTTTTgTATgAgTgCCgTA ACCTTggCCATCCTCTTCTT AgAAATCTTgACCCCCgTTC AAggAgCTCTTgACgTTggA TgCTAACAggTgggAAAACC CCTTCCAgCAgATgTggATT AgATTAggCAAggCgAggAT BEC87-GSP1 ... TgATTTTAgCTgCATgTgCACCTgAgAA CgTCATggAAATgTCTTAATTggCTTgCTg gAAgAAAACAAATTgggAggAggAgAA gCgTgTTCCAAAgAACACAATTCAgTgCCTT BEC-32BamHI DX24BamHI ggATCCTgATCTgTggATTgggTTTCgTgg ggATCCgggTgTTgAACCAAAATgCgCCATT Method RT-PCR ... THA82-GSP1 THA-1-GSP2 LOX101-GSP1 LOX63-GSP2 TgCATTTCCAgCTTgCCTCCCACATTg CTgAgATCCCTAACAgCAAAgCTAgggATA ACCggTTCCggTggTggTgTgATgAACC ACTCATCAgTCTTAgTAggCTCgggTgTT TgATTTTAgCTgCATgTgCACCTgAgAA...
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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... stages of seed development, and which is the only LOX detected in pea leaves [25] Considering the high level of expression of CaLOX2 in hazelnut seeds, it would be of interest to isolate and characterize ... characterize hazelnut lipoxygenases at the biochemical and molecular level Materials and methods Plant material Hazelnut (Corylus avellana L cv Mortarella) leaves and seeds were harvested at different...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 2003 Laccase gene from Volvariella ... min; then a final extension at 72 °C for 10 The primers for lac1 PLAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢) and PLAC1R (5¢-AACGAG CTCAAGTACAAATGACT-3¢) were designed according to our cloned cDNA (GenBank...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... protein synthesis in the cell-free system of rabbit reticulocyte The protein synthesis decreased gradually with the increment of lamjapin in the reaction mixture The IC50 (the concentration of ... problem, the phenyl-Sepharose CL-4B column chromatography was chosen as the first step and it separated efficiently the target proteins from the inhibitory substances (Table 1) Proteins of peak from the ... in the pH range 3–10 and the proteins were stained with Coomassie Brilliant Blue by the method of Neuhoff et al [20] The pI was determined by calculating the linear regression of the marker proteins...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Purification of beta glucosidases from Talaromyces emersonii ... classification of cellulases Eur J Biochem 258, 200–206 68 Takashima, S., Iikura, H., Nakamura, A. , Hidaka, M., Masaki, H & Uozumi, T (1998) Isolation of the gene and characterization of the enzymatic...
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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx
... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... kDa), NdhE (12 kDa), NdhF (83 kDa), NdhG (18 kDa), NdhH (45 kDa), NdhI (21 kDa), NdhJ (18 kDa), NdhK (29 kDa) The 75, 51 and 23 kDa subunits are assigned as the soluble subcomplex of the Ndh complex...
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Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 docx
... alignment of the N-terminal sequence of ChPLA2 with those of TPLA2 [14], OPLA2 [15], DrPLA2 [13], PPLA2 [20], bovine PLA2 [20], and human PLA2 [21] The N-terminal sequences of bird PLA2s exhibit ... TPLA2, OPLA2 DrPLA2, bovine PLA2 and human PLA2 Identical amino acids are in bold, and homologous amino acids are in italic ChPLA2 PPLA2 TPLA2 OPLA2 DrPLA2 Bovine PLA2 Human PLA2 :ALWEF :ALWQF ... that of known mammal PLA2s Molecular modeling was also proposed to explain some biochemical differences between ChPLA2 and other pancreatic PLA2 Results and discussion Purification of ChPLA2 Thirty...
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Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx
... were eluted in two phases with 300 mL of linear gradient of 0–0.6 M NaCl and 50 mL of linear gradient of 0.6–1 M NaCl at a flow rate of 0.8 mLÆmin)1 Fractions of mL were collected and those displaying ... results of studies leading to the conclusion that at least two distinct soluble enzymes of P denitrificans exhibit an activity of Fe(III) reductase These enzymes were purified and their molecular and ... In this study, we have demonstrated a coexistence of two types of soluble Fe(III) reductases, FerA and FerB, in the soil bacterium Paracoccus denitrificans Both enzymes are flavin-dependent, but...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene sequences in transgenic plants Results Use of FTA for sampling, retrieval and PCR-based ... Effective methods for sampling, storage and retrieval of viral pathogens from infected plant tissues allows not only identification of the viral pathogens but also detailed molecular study of their genomes, ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx
... of infectious bronchitis virus isolated in the UK Vet Rec 2008, 162, 99-100 11 Gough RE, Randall CJ, Dagless M, Alexander DJ, Cox WJ, Pearson D A ‘new’ strain of infectious bronchitis virus infecting ... region of the S1 gene of infectious bronchitis virus Arch Vrol 2000, 145, 291-300 Yu L, Wang Z, Jiang Y, Low S, Kwang J Molecular epidemiology of infectious bronchitis virus isolates from China and ... FJ156080 Bird Age Organs used for type (days) virus isolation Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler Broiler 28 30 26 28 20 20 20 26 16 22 28...
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báo cáo khoa học: " Isolation and functional characterization of CE1 binding proteins" pptx
... proteins CEBFs (CE1 binding factors) DNA -binding and transcriptional activities of CEBFs Binding of a number of CEBFs, which were isolated as multiple isolates (Table 1), to the CE1 element was ... Lee et al.: Isolation and functional characterization of CE1 binding proteins BMC Plant Biology 2010 10:277 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... 1000 1200 -galactosidase activity Figure Binding and transcriptional activities of CEBFs (A) Binding of CEBFs to the CE1 element The binding activity of six CEBFs was confirmed in yeast Reporter...
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Báo cáo y học: "Serological and molecular expression of Hepatitis B infection in patients with chronic Hepatitis C from Tunisia, North Africa" pdf
... resolutive HBV infection, HBV/HCV co-infected with overt HBV infection (HBsAg positives) and < /b> HBV/HCV co-infected with occult HBV infection (HBsAg negatives) The 30 patients with uncertain HBV status ... E, Coppola N, Scolastico C, Filippini P, Santantonio T, Stroffolini T, Piccinino F: Virologic and < /b> clinical expressions of < /b> reciprocal inhibitory effect of < /b> hepatitis < /b> B, C, and < /b> delta viruses in patients ... 22.5% of < /b> occult HBV infection in 71 patients with chronic HCV infection and < /b> HBcAb positives These results are similar to the rates found herein if we consider only patients with HBcAb positivity...
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