Generation of hepatocyte like cells from mouse embryonic stem (ES) and bone marrow (BM) cells
Generation of hepatocyte like cells from mouse embryonic stem (ES) and bone marrow (BM) cells
... Generation of Hepatocyte- like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF ... ENTITLED Generation of Hepatocyte- like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells The paucity of hepatocytes available for hepatocyte replacement therapy requires consideration of ... type of stem cell is the adult stem cells Like ES and EG cells, adult stem cells can self-renew, but unlike ES and EG cells, they give rise to specific sets of mature cell types Adult stem cells...
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Báo cáo y học: " Expression of Toll-like Receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis" ppsx
... events bone marrow TLR9-FITC Figure of TLR9 in leukocytes from bone marrow, peripheral blood and nasal lavage fluid Expression Expression of TLR9 in leukocytes from bone marrow, peripheral blood and ... plots of intracellular staining of TLR9 in neutrophils, eosinophils, basophils, monocytes, lymphocytes and immature granulocytes Expression of TLR9 in leukocytes was analyzed by flow cytometry using ... http://respiratory-research.com/content/8/1/17 A B C Figure Expression of TLR9 in leukocytes during allergic rhinitis Expression of TLR9 in leukocytes during allergic rhinitis Intracellular expression of TLR9,...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... transplanted vascular cells derived from human ES cells and the vascular density in the infarct area after the transplantation In the saline- and hMNCs-injected groups, the vascular density of host ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... The findings reported here demonstrate that the transplantation of vascular cells, ECs and MCs derived from human ES cells, to the ischemic brain significantly promoted vascular regeneration in...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... phosporylation of MAPKs proteins may result in the prevention of downstream events such as activation of NF-κB [44] Therefore, we suggest that the Effect of dihydrotestosterone on mouse embryonic stem cells ... sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress ... Therefore, the purpose of this study was to determine the effect of DHT on mouse ES cells under conditions of H2O2-induced oxidative stress Materials and Methods Materials The mouse ES cells were obtained...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... of data, data analysis and interpretation, manuscript writing and final approval of the manuscript AK was involved in manuscript writing and data analysis SL was involved in the conception and ... of study materials, collection and assembly of data, data analysis and interpretation, manuscript writing and final approval of the manuscript XH Participated in the collection and assembly of ... support, administrative support, provision of study materials, collection and assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript as well as serves as...
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Báo cáo y học: "A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology" ppt
... adherent monocytes using the Pittsburgh Protocol in roller bottle cultures in Generation Generation of phenotypically mature mDC from adherent monocytes using the Pittsburgh Protocol in roller bottle ... flasks Roller bottles Roller bottles Figure static flask of phenotypically mature mDC from adherent monocytes using ITIP in roller bottle cultures in comparison to Generationcultures Generation of ... flasks Roller bottles Figure Phenotypic analysis of DC purity in non-matured DC cultures from roller bottle and static flask cultures Phenotypic analysis of DC purity in non-matured DC cultures from...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic applications ... Daley GQ: Therapeutic potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embryonic stem cells: A system to study early human ... colony-forming cells derived from humanembryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Vodyanik MA, Bork JA, Thomson JA, Slukvin II: Human embryonic stem cell-derived CD34+ cells: ...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... analyze the impact of different chromatin modifiers on the replication timing profile of mouse ES cells We show that, while early replication in ES cells correlates with peaks of increased histone ... staining and cell cycle profiles of the mutant ES cell lines analysed Supplementary Figure contains replication timing profiles of all loci analysed in each of the mutant ES cell lines analysed ... Collectively, these data suggest that only a minority of loci (5/23; supplementary Figure in Additional data file 2) change their replication timing in response to severe reduction of DNA methylation...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... plating of hESC-derived chondrogenic cells Fig 17 Chondrogenic differentiation capability of hESC-derived chondrogenic cells Fig 18 Analysis of pluripotency and lineage- restriction of hESC-derived chondrogenic ... explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hESCs) are stem cell lines of embryonic origin, isolated from ... biomaterial and biophysical stimulations have been employed to harness the chondrogenic potential of hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Optimisation of the Oct4 and Sox2 ChIP assays 57 3.2.2 Oct4 and Sox2 bind to the distal enhancer of Oct4 in mESCs 58 3.2.3 Oct4 and Sox2 bind to the SRR2 of Sox2 in mESCs 59 3.2.4 Oct4 and Sox2 bind to ... Specificity of Oct4 and Sox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 binding reduces after retinoic acid differentiation of mESCs 3.4 Oct4 and Sox2 ... Characterization of the Sox2 -Oct4 DNA binding motif 119 6.2.4.1 Interactions of Sox2 and Oct4 with the Sox2 -Oct4 joint motifs 6.2.4.1.1 Sox2 and Oct4 bind to the Sox2 -Oct4 DNA motif in vitro 119...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... 35 04 - 36 02 139 - 22 6 20 7 - 31 3 45 0 - 559 11 13 - 130 8 1 637 - 1 8 32 1559 - 1665 39 9 - 5 92 3. 60 3. 62 3. 99 4 . 34 4. 78 3. 68 3. 26 3. 4 3. 4 32 .8 29 .25 30 .7 30 .8 35 .8 27 .2 29.1 28 .3 30.8 Bold designates ... Sox- 2 297 – 37 8 5 73 – 677 1056 - 11 53 71 - 23 0 11 82 - 1 34 5 4. 55 2. 98 3. 68 3. 49 4 .36 19 .4 33 .9 28 .2 36 .7 34 .3 Fgf -4 Utf-1 Utf-1 Fbx-15 Fbx-15 Opn Opn 5 52 - 633 38 2 - 644 865 - 10 63 2 14 - 30 5 866 ... 6 82 880 - 969 3. 3 Poor Amplification 4. 07 3. 20 3. 40 3. 70 3. 76 N/A 22 .9 28 .1 33 .8 25 .3 33. 5 29 .2 Nestin Nestin Ptx -3 Ptx -3 Ptx -3 Brachyury Brachyury Gata -4 Gata -4 11 62 - 130 5 35 04 - 36 02 139 - 22 6...
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Báo cáo hóa học: "Generation of Embedded Hardware/Software from SystemC" docx
... synthesizable HDL from highlevel C/SystemC specifications The Agility Compiler from Celoxica can help the generation of synthesizable VHDL from SystemC The final product of the design flow is a set of binary ... with the idea of unifying the use of SystemC to implement both hardware and embedded software We propose an automated HW/SW codesign methodology which reduces the design time of embedded systems ... Proceedings of 22nd Norchip Conference, pp 218–221, Oslo, Norway, November 2004 [30] S Ouadjaout and D Houzet, Embedded hardware/software generation from high level design languages,” in Proceedings of...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
... established a cell bank consisting of mouse embry- Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes ... long-term overexpression of the transgene in a dose- and time-dependent manner It is therefore suitable for systematic expression of HSA21 cDNAs Cell bank: the HSA21 gene collection in mES cells HSA21 ... transcriptional profile obtained on the 'transchromosomic' Tc1 mouse line [26] The Tc1 ES cells carry an extra copy of HSA21 and they represent a reference model of trisomy 21 for which publicly accessible...
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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt
... Billon et al.: Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development Genome Biology 2010 11:R80 Submit your next manuscript ... Maintenance of pluripotential embryonic stem cells by stem cell selection Reprod Fertil Dev 1998, 10:527-533 53 Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of ... pathways and intermediates between the embryonic stem cell and the mature adipocyte In particular, mouse embryonic stem cells (mESCs) have provided an invaluable tool to model the earliest steps of...
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Báo cáo y học: "Rapid generation of an anthrax immunotherapeutic from goats using a novel non-toxic muramyl dipeptide adjuvant." docx
... goat anti-sera Cell viability determined by an MTT-based assay A Anti-PA83 IgG Data shown are the average ± SEM of five assays each with four replicates EC50 is 2.57 × 10-7 M B Anti-PA83 F(ab')2 ... volumes of antisera Additionally, the goats can be plasmapheresed up to four times per year for several years making for a nearly endless source of antisera Plasmapheresis of three goats generated ... complement-mediated lysis of HIV-1 by PEHRG214, a novel caprine anti-HIV-1 polyclonal antibody AIDS 2006, 20:505-515 Ahuja N, Kumar P, Bhatnagar R: Rapid purification of recombinant anthrax- protective antigen...
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Từ khóa: karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell linescollection and processing of peripheral blood stem cells and bone marrow pdfisolation of high quality rna from embryonic kidney and cellsgeneration of functional islets from human umbilical cord and placenta derived mesenchymal stem cellsautomatic generation of domain models for call centers from noisy transcriptionsindirect immunostaining on mouse embryonic heart for the detection of proliferated cardiomyocyteNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI