Molecular analyses of gonad differentiation and function in zebrafish

... help in the study of gonad differentiation in zebrafish Studying gene function utilizing widely used molecular tools, such as gene knock-out, morpholinos, and cell culture, is also difficult in zebrafish ... during gonad differentiation: foxl2, cyp19a1a, cyp11a, hsd3b and cyp17a1 in ovarian differentiation and star, nr5a1a and cyp11b2 in testicular differentiation In order to broaden the understanding ... examine the gonad development 17 To explore the dynamic process of gonad differentiation, three transgenic zebrafish lines expressing EGFP in their gonads have been generated In one of these lines,...

Báo cáo y học: " Molecular analysis of HBV genotypes and subgenotypes in the Central-East region of Tunisia" potx

... al.: Molecular analysis of HBV genotypes and subgenotypes in the Central-East region of Tunisia Virology Journal 2010 7:302 Submit your next manuscript to BioMed Central and take full advantage of: ... NH and OB conceived of the study, participated in its design, and in drafting the manuscript HT and JB participated in the study design and coordination and in discussing manuscript NH, NBF and ... suggested the predominance of this new subgenotype in the region [23,28] Subgenotype D1 is Page of predominant in the Eastern part of Africa; Saudy et al described it in Egypt [29] Our region geographically...

Báo cáo y học: "Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications" ppsx

... development and application of non invasive in vivo methodologies to the study of biological structure, function and xenobiotic response in STII medaka The development of this in vivo investigatory "system" ... study of piscine biliary transport Collectively these findings demonstrate the ability to study, with high resolution, normalcy and disease/toxicity in vivo in the hepatobiliary system of living ... hepatobiliary architecture Non invasive in vivo imaging in STII medaka allowed the generation of 3D models of the hepatobiliary system (Movies – 9), under conditions of normalcy and toxicity Using LSCM,...

Molecular simulation of gas permeation and separation in polymer membranes

... understanding of gas permeation and separation in the two PIMs is useful for the new development of polymer membranes with high permeability and selectivity vi Permeation and separation of CO2 and ... elucidate gas permeation and separation in two classes of newly synthesized polymer membranes, namely polymers of intrinsic microporosity (PIMs) and polymerized ionic liquids (PILs) These polymer membranes ... polymer membranes have been increasingly used for gas separation in industry The business of polymer membrane-based gas separation increased from Chapter Introduction 120 M$ in 1996 to 250 M$ in 2000...

Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

... examined for its gonadinhibiting function by using a RNA interference (RNAi) technique RNAi, a post-transcription gene-silencing process in which dsRNA triggers sequence-speciﬁc suppression of its cognate ... that of Mee-GIH expression in mature female M ensis [17] Interestingly, expression of Pem-GIH in 972 these tissues was found in both male and female of adult and adolescent P monodon (Fig 4) dsRNA-induced ... automated DNA sequencing The recombinant plasmid of hairpin -RNA of Pem-GIH was subsequently transformed into an RNaseIII-deﬁcient E coli HT115 strain Expression of hairpin -RNA was induced with 0.4...

báo cáo khoa học: " ''''Who''''s who'''' in two different flower types of Calluna vulgaris (Ericaceae): morphological and molecular analyses of flower organ identity" pdf

... understanding of mutations in flower morphology in this crop, an initial cloning of two MADS-box genes was realized in addition to preliminary expression analyses The genome size was determined in ... uncertain, whether stamen development is been initiated or whether the initiation of primordia is inhibited The determination of the flower organ identity and the understanding of the development of ... microscopic and molecular examinations to clarify the identity of flower organs and of existent differences between wild-type and 'budflowering' phenotypes Results In order to elucidate the unknown organ...

Analysis of gonad differentiation in zebrafish by histology and transgenics

... ANALYSIS OF GONAD DIFFERENTIATION IN ZEBRAFISH BY HISTOLOGY AND TRANSGENICS WANG XINGANG (B Sc., Ocean University of Qingdao, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... be on discussing the mode of sex determination and candidate genes involved in gonad differentiation of zebrafish 1.2 Sex determination mechanism of some invertebrates (fruit fly and worm) The ... absence of a sex linked marker zebrafish cannot be sexed, which hinders the studies of molecular mechanism of gonadal differentiation Traditional method using Hematoxylin and Eosin staining requires...

Báo cáo y học: "Advances in Molecular Diagnosis of HBV Infection and Drug Resistance"

... in the HBV genomic sequence in the course of treatment In addition, indirect measures of lamivudine resistance including determination of ALT values, and especially HBV DNA (viral load) assays ... within a broadening and evolving clinical context Moreover, it is increasingly apparent that clinically relevant prediction and monitoring of viral resistance may not only require the use of existing ... generally defined clinically by a rise in serum HBV DNA levels during antiviral therapy Although HBV DNA assays will eventually detect changes in viral load as a consequence of emerging resistance...

Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threeﬁnger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx

... proteomics and variations of Bf venom, we studied individual venom of two specimens of Bf from Kolkata, India (designated as KBf) by a comparative proteomic and genomic approach The venom PLA and 3FTx ... structure of an inactive mutant of phospholipase A2 in the venom of Bungarus fasciatus (banded krait) J Biochem (Tokyo) 112, 707–713 Venom proteins of Bungarus fasciatus Liu CS, Chang CS, Leu ... high Ca2+ binding afﬁnity Finally, phylogenetic analyses of the elapid PLAs and the krait 3FTx were carried out to Venom proteins of Bungarus fasciatus better understand the intrageneric and intergeneric...

Báo cáo hóa học: " Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies" doc

... Cite this article as: Bonanno et al.: Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies Journal of Translational Medicine 2010 8:114 Submit ... implication of our findings pertains to HLA-matched stem cell transplantation, a clinical context where pegfilgrastim administration may modulate the number of immune cells and/ or levels of immune ... (Th2) cytokine production [3] In humans, G-CSF increases interleukin (IL)-4 release and decreases interferon (IFN)-g production [4], induces immune modulatory genes in T cells, including the Th2...

Báo cáo y học: "Impact of cytokines and T lymphocytes upon osteoclast differentiation and function" pot

... addition to cytokines that are expressed by T lymphocytes to inhibit osteoclast formation, several cytokines have been reported to act upon T lymphocytes, affecting their action upon bone These ... several cytokines and growth factors that are secreted by, or modulate the activities of, T lymphocytes also affecting osteoclast formation and activity Of interest now and for future studies is the ... the essential contribution of T cells to bone loss In addition to the ability of T lymphocytes to directly support osteoclastogenesis, T lymphocytes also secrete proresorptive cytokines, including...

Báo cáo y học: "Leukocyte numbers and function in subjects eating n-3 enriched foods: selective depression of natural killer cell levels" potx

... processed with Lysis II software (BD Biosciences) To determine leukocyte and lymphocyte numbers, 130 μl of whole blood was aspirated and analyzed using a hematology analyzer CELL- DYN 3500SL (Abbott ... LCPUFA levels in erythrocyte membranes and LT production by lymphocytes The discrepancy in the pattern of regression – that is, linear in the case of LT or U-shaped in that of PHA-induced proliferation ... 8.04% The reduced NKcell numbers can only be contributing in a very small way to the reduced levels of total leukocytes and lymphocytes seen over this range of n-3 LCPUFA, EPA and DHA levels While...

Báo cáo y học: " Effects of cyclophosphamide on pulmonary function in patients with scleroderma and interstitial lung disease: a systematic review and meta-analysis of randomized controlled trials and observational prospective cohort studies" ppt

... examined the benefit of CYC on lung function as measured by pulmonary function tests by conducting a systematic review and meta-analysis of randomized clinical trials and prospective observational ... conceived the study and participated in its design, coordination, data acquisition and analysis, and in manuscript preparation CPW and ELM participated in the study design, data acquisition and ... and analysis, and in manuscript preparation PJE participated in data acquisition and in manuscript preparation All authors read and approved the final manuscript Additional files The following Additional...

Báo cáo khoa học: "Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx

... characterization and analysis of isolation of Sin Nombre virus Journal of Virology 1995, 69:8132-8136 Huang C, Campbell WP, Means R, Ackman DM: Hantavirus S RNA sequence from a fatal case of HPS in New York ... test of7 neutrality (Fu and Li, 1993) for the M and S segments F test of neutrality (Fu and Li, 1993) for the M and S segments Only sequences from clade of the M tree (Figure 3) and clades – of ... locations of trapping sites at which rodents with Sin Nombre virus RNA were obtained Map of Map of western United States showing locations of trapping sites at which rodents with Sin Nombre virus RNA...

Báo cáo khoa học: " Temporal and geographic evidence for evolution of Sin Nombre virus using molecular analyses of viral RNA from Colorado, New Mexico and Montana" docx

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