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Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

Quantification of biomolecule dynamics and interactions in living zebrafish embryos by fluorescence correlation spectroscopy

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion  model and study the molecular crowdedness of cells in living embryos.   Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with  ... use  in zebrafish embryos and demonstrate  several  applications of FCS in living zebrafish embryos,  showing that single molecule‐based  studies in living organism are possible:  The  autofluorescence  ... foreign  genes  in zebrafish embryos are  discussed  and compared  with that in cell cultures. Secondly, the autofluorescence in living zebrafish embryos,   in particular  the  autofluorescence ...
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Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy

Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy

... for studying rafts is interrupting rafts by changing the amount of cholesterol in cells, which includes increasing its amount by adding in extra cholesterol and extracting it by drug treatments ... thereby enabling the exposure of the ligand-binding pocket of domain L1 and L2 The dimerization arm in domain CR1 is known to facilitate the formation of dimers, which leads to the kinase domain of ... functions of EGFR such as binding with ligands and subsequent activation Interestingly, again, opposing effects were observed in different settings of experiments In artificial 20 membranes, the binding...
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Characterization of diffusion behavior of a novel extra cellular sphingolipid associated peptide probe by fluorescence correlation spectroscopy and imaging total internal reflection fluorescence correlation spectroscopy

Characterization of diffusion behavior of a novel extra cellular sphingolipid associated peptide probe by fluorescence correlation spectroscopy and imaging total internal reflection fluorescence correlation spectroscopy

... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectroscopy (ITIR-FCS) and Imaging Total Internal Reflection -Fluorescence ... Thorsten Wohland, and Rachel Kraut Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative vs recycling pathways Journal of Cell Science...
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Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)

Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term first introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identified points of interest within a sample and ... determine protein dynamics and molecular interactions The use of two SPCMs in combination with a 50:50 non-polarizing beamsplitter allows the cross correlation measurement of SPCM signals eliminating...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY

INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our approach promises to shed light on the biophysical features of cellular ... this is the first time confocal FCS is being used to study membrane fluidity in situ in the central nervous system of Drosophila melanogaster embryos A study by Sergent et al indicated that increased...
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Investigation of peptide lipid interaction by fluorescence correlation spectroscopy

Investigation of peptide lipid interaction by fluorescence correlation spectroscopy

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9.          I    Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy   List of Figures  Fig 1.1 Schematic representation of the Gram‐negative bacteria cell wall.  ... Phase of clinical trial     Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy   Table 1.1 Clinical developments of cationic antimicrobial peptides      14      Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ...
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Quantification of vitamin e and ç oryzanol components in RiceGermandBran

Quantification of vitamin e and ç oryzanol components in RiceGermandBran

... The profiles of free vitamin E and γ -oryzanol in RG and FFRB differ significantly The levels of free vitamin E and γ -oryzanol are the same as in RG, but the level of free vitamin E is 27 times ... analyses Calibration curves of each standard were created from seven concentrations using Microsoft Excel software The concentrations of individual components in the rice samples were determined ... Figure Structures of vitamin E and γ -oryzanol components examined in this study However, commercial rice bran is a mixture of rice bran and rice germ (20% of rice bran), and the difference in the...
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báo cáo hóa học:

báo cáo hóa học: " Quantification of functional weakness and abnormal synergy patterns in the lower limb of individuals with chronic stroke" pdf

... for the first time is a quantitative analysis of lower limb weakness and synergy patterns of chronic stroke subjects in a functionally relevant standing weightbearing position In a standing position ... flexion of the hip, flexion of the knee, and flexion and eversion of the ankle) [16,17] with varying levels of completeness [18] and dominance [19] Much of the literature attempting to quantify these ... extension synergy (internal rotation, adduction, and extension of the hip, extension of the knee and extension and inversion of the ankle) and the flexion synergy (external rotation, abduction, and...
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Báo cáo y học:

Báo cáo y học: "The trauma of ongoing conflict and displacement in Chechnya: quantitative assessment of living conditions, and psychosocial and general health status among war displaced in Chechnya and Ingushetia" pptx

... of the general and psychosocial health status of Chechnen's internally displaced The self-reported health conditions and the general health questionnaire showed high levels of medical and psychosocial ... returned internally displaced within Chechnya As a consequence of Methods Two surveys were executed: one in Ingushetia (January 2004) and one in Chechnya (February 2004) A systematic sampling method ... developing health (including mental health) problems [8-10] Generally speaking the proximity to the event [11-13], the severity of the incident [14], and the extent of the physical injury increases...
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investigation and quantification of codon usage bias trends in prokaryotes

investigation and quantification of codon usage bias trends in prokaryotes

... usage bias 12 2.2.1 Evolutionary causes of codon usage bias 13 2.2.2 Types of codon usage bias 14 2.2.3 Quantifying codon usage bias 17 Exploration of codon usage bias ... the lagging strand This type of bias is known as GC-skew 2.2.3 Quantifying codon usage bias The goal of methods for quantifying and representing biased codon usage is to indicate which codons ... School of Graduate Studies ABSTRACT Hanes, Amanda L M.S., Department of Computer Science and Engineering, Wright State University, 2009 Investigation and quantification of codon usage bias trends in...
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Studies on structures, dynamics and interactions with small molecules of CNS regeneration inhibitory components associated with nogo a and epha4

Studies on structures, dynamics and interactions with small molecules of CNS regeneration inhibitory components associated with nogo a and epha4

... study structures, dynamics and protein-protein interactions, as well as protein -small molecule interactions for two CNS regeneration inhibitors: Nogo- A and EphA4 receptor Intracellular Nogo- A protein ... solution structure of EphA4 LBD 132 3.5.5 Comparison of NMR solution structure and crystal structure 137 3.6.6 Dynamics study of free EphA4 and analysis of relaxation data 139 3.7.7 Modelfree analysis ... ligand binding domain with compound 1and compound Figure 3.25 Characterization of the interactions with two small molecule antagonists Figure 3.26 Models of EphA4 (chainA) in complex with small...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy

The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy

... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing proteins Adaptor proteins with a SH2-binding domain ... ligands bind to the extracellular domains of two receptor subunits and form a 2:2 complex (42), resulting in activation of the intracellular kinases of the receptors Upon ligand binding, the...
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The dynamics of literary representation and interpretation in a multilingual environment  a study of selected malaysian and singaporean novels in english

The dynamics of literary representation and interpretation in a multilingual environment a study of selected malaysian and singaporean novels in english

... THE DYNAMICS OF LITERARY REPRESENTATION AND INTERPRETATION IN A MULTILINGUAL ENVIRONMENT: A STUDY OF SELECTED MALAYSIAN AND SINGAPORE NOVELS IN ENGLISH ROSALY JOSEPH PUTHUCHEARY (M .A, in English ... language and mother tongues as the second The National Language (Bahasa Kebangsaan) which is standard Malay became gradually the medium of instruction in all schools in Malaysia after 1976 Although English ... was slow and somewhat erratic, it is not surprising that the main lingua franca among the non -English educated remained Bazaar Malay, while among the English educated, a new lingua franca, Singapore-Malayan...
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In vivo aspects of potential stereospecific drug interactions of oral warfarin and rutin in rats

In vivo aspects of potential stereospecific drug interactions of oral warfarin and rutin in rats

... activity of the drug But metabolite estimation helps in understanding the source of variation and mechanism of interaction of drugs with warfarin A better understanding of the warfarin drug interactions ... Mean S- warfarin curve fitting for (a) control and (b) rutin treated rats using WinNonlin 71 4.3 Mean R- warfarin curve fitting for (a) control and (b) rutin treated rats using WinNonlin 72 4.4 ... rutin treated rats in single dose study using WinNonlin 49 3.3 R- warfarin (mean) curve fitting for (a) control and (b) rutin treated rats in single dose study using WinNonlin 50 3.4 Changes in...
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