Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems

... illustrating prevention of Blastocystis- induced ZO-1 and actin rearrangement in Caco-2 monolayers by ROCK and HMG CoA reductase inhibition 151 Fig 5.6 Quantification of ZO-1 staining in Blatocystis-infected ... kinase inhibition 5.3.3 Blastoycstis induces Rho kinase-mediated phosphorylation of Myosin Light Chain at ser-19 position 5.3.4 HMG CoA-reductase inhibition in Caco-2 prevents Blastocystis- induced ... Role of Blastocystis cysteine proteases in ST-7 (B)-induced increase in Caco-2 permeability 128 Fig 4.7 Representative confocal micrographs of alterations in ZO-1 and F-actin organization in Caco-2...

Blastocystis investigations on host pathogen interactions using in vitro model systems

... preparation 4.2.3 Experimental planning and inoculation protocol 4.2.4 DAPI staining for nuclear fragmentation and condensation 4.2.5 Annexin V binding assay for expression of phosphatidylserine molecules ... and concentrations Table 3.1 Percentage of secretory IgA intact heavy chain remaining 75 after incubation with the lysates and conditioned medium of Blastocystis Table 3.2 Degrees of inhibition ... downregulate host immune responses in order to improve survival in the gut Other isolated clinical reports described intestinal inflammation and edema in patients infected with Blastocystis (Kain et...

Báo cáo y học: " Cysteinyl-leukotrienes in the regulation of β2-adrenoceptor function: an in vitro model of asthma" ppsx

... production, and certainly involve the activation of other components downstream of the receptor, while the β2-AR may perform functions other than adenylyl cyclase activation [28], yet equally involved in ... adenylyl cyclase was not directly affected by LTD4 Relaxant responses to salbutamol in human bronchial rings The mean weight of the 24 bronchial rings was 91 ± mg The mean resting force and the ... the HASMC themselves [9] upon exposure to allergen Among these mediators, cysteinyl-leukotrienes (cysteinyl-LTs) are long known to play an important role in asthma [10,11] Cysteinyl-LTs originate...

Báo cáo sinh học: " In-vitro model systems to study Hepatitis C Virus" ppt

... models currently used to study future HCV research as well as drug design HCV Infection System In the past, research into HCV has been hampered due to lack of a reliable cell culture system Conventional ... month and HCV core protein and HCV negative-strand RNA also detected in infective cells Viral replication had some cytotoxic effects on the cells due to production of interferon as a component ... http://www.gvt-journal.com/content/9/1/7 permissive to JFH-1 virus infection [31] JFH-1 infectious particle is an ideal tool to study all aspects of the HCV life cycle including viral attachment, entry, trafficking,...

Báo cáo vật lý: "Electrochemical Studies of Mild Steel Corrosion Inhibition in Aqueous Solution by Uncaria gambir Extract" doc

... Electrochemical Studies of Mild Steel Corrosion Inhibition Table 4: Corrosion parameters for mild steel in aqueous solution with absence and presence of various pH of 150 ppm ethyl acetate extracts of U gambir ... for mild steel in pH aqueous solutions with absence and presence of various concentration of ethyl acetate extract of U gambir Table 3: Corrosion parameters for mild steel in pH aqueous solution ... layer being formed to stop further corrosion. 10,11 Table 1: Inhibition efficiencies for various concentration of ethyl acetate extract of U gambir for the corrosion of mild steel in pH aqueous solution...

Báo cáo y học: "Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model" pdf

... very difficult to start giving repeated doses of BCG in India without any experimental studies The aim of the present study is to investigate the immune responses to repeated stimulation of PBMC' s ... were again washed and resuspended in ml saline and subjected to flowcytometry Cells collected using flow cytometry on a FACS were analyzed using FlowJo software by gating on the lymphocyte population ... Kashyap et al., Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model Journal of Immune Based Therapies and Vaccines 2010, 8:3 Page of ...

Topological studies of circular and elliptic jets in a cross flow

... the characteristics of non -circular jet in a cross flow In all cases, the cross flow was maintained in a laminar condition and qualitative flow visualization and quantitative particle image velocimetry ... et al (2000), Hasselbrink and Mungal (200 1a, 2001b), Rivero et al (2001), Cortelezzi and Karagozian (2001) and Gollahalli and Pardiwalla (2002) One of the main reasons why analysis of this particular ... for Circular Jet in a Cross Flow 73 CHAPTER : Flow Visualization of Elliptic Jets in Cross Flow 6.1 Introduction 85 6.2 Low Aspect Ratio Elliptic Jets in Cross Flow 88 6.3 High Aspect Ratio Elliptic...

Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt

... support DNA replication of a plasmid carrying the JCV origin Columns and 3, SV40 Tag with human and murine DNA polymerase a-primase, respectively; columns and 5, JCV Tag with human and murine DNA polymerase ... slightly inhibited by the murine enzyme complex Fig Autoradiogram of an in vitro JCV DNA replication initiation assay with 0.2 and 0.4 units of primase of either human (H4) or murine (M4) DNA polymerase ... containing the SV40 origin of DNA replication (B) The JCV system uses JCV TAg and pJC389 DNA with the JCV origin The cell extracts were supplemented with 0.5 and 1.0 DNA polymerase units of the indicated...

Báo cáo khoa học: RNA reprogramming of a-mannosidase mRNA sequences in vitro by myxomycete group IC1 and IE ribozymes pptx

... RNA reprogramming of a-mannosidase mRNA sequences RNA reprogramming in cell cultures based on the Tetrahymena ribozyme remains inefficient in spite of significant efforts to increase the ... ribozymes, were identified and included in site-directed RNA reprogramming Results and Discussion Structural features of the myxomycete group IC1 and group IE ribozymes The intron secondary-structure ... Group IC1 and group IE ribozymes select the same accessible sites in an a-mannosidase mRNA sequence Deficiency of lysosomal activity of human a-mannosidase, an exoglycosidase enzyme involved in...

Báo cáo khoa học: Identiﬁcation of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf

... Here, we report the partial characterization and the identiﬁcation of three L amazonensis proteins that bind in vitro to the telomeric G -strand (L amazonensis G -strand telomeric proteins; LaGT1, ... pretreated with 10 lg of proteinase K, indicating that they are indeed formed by the interaction of proteins and DNA (data not shown) UV cross-linking assays were carried out to estimate the size of the ... represent the percentage of the binding activity of a shifted complex (the ratio of the density area in arbitrary scanning units, and the sum of the density areas of all shifted complexes, including...

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... by a nearest-neighbour thermodynamics calculation, suggesting that it can be useful for evaluating the reopening temperature for a DNA hairpin in a local region of DNA, and for comparing the reopening ... measuring the reopening temperature for a single type of hairpin structure in DNA has been established The reopening temperature obtained experimentally was fairly close to the Tm values obtained ... either slow (Fig 2B, lane 3) or fast (Fig 2B, lanes and 4) annealing These data taken together indicate that the hairpin- forming region (as indicated in Fig 1A) can indeed affect primer DNA primer...

Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot

... that pseudotyped virus comprising a lentivirus, which does not require replicating host cells for integration of its genome, together with an envelope containing vesicular stomatitis virus glycoprotein ... chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell genomes and infect a broad range of cell-types The caveat to the preceding statement ... histocompatibility molecules thereby preventing recognition by cytotoxic T cells Antibodies on the other hand retain their ability to recognize native antigens but in case of tumors their potential...

báo cáo hóa học: " Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve " doc

... al.: Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair Journal of NeuroEngineering and Rehabilitation 2010 ... environment during axonal regeneration In the present study, we aimed at investigating the effects of enwrapping the site of end-to-end rat sciatic nerve repair with equine type III collagen nerve membranes ... either alone or enriched with N1E-115 pre-differentiated into neural cells in the presence of 1.5% of DMSO The rationale for the utilization of the N1E-115 cells was to take advantages of the properties...

báo cáo hóa học: " A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices" potx

... well to pro and anti -inflammatory stimuli during the culture Next, we wanted to see if either proinflammatory histone deacetylase inhibitor TSA or anti -inflammatory NF-κB inhibitor helenalin could ... Tanaka J, Toku K, Matsuda S, Sudo S, Fujita H, Sakanaka M, Maeda N: Induction of resting microglia in culture medium devoid of glycine and serine Glia 1998, 24:198-215 Nakamura Y, Si QS, Kataoka ... Potentiation and downregulation of LPS-induced inflammatory response by TSA and helenalin Exposure time was 24 h at DIV LPS concentration was µg/ml, TSA concentration was 20 nM and that of helenalin 0.5...

báo cáo hóa học:" Assessment of the primary rotational stability of uncemented hip stems using an analytical model: Comparison with finite element analyses" potx

... designs, the resistance against axial rotation was assessed using FEM on the one hand and the analytical model on the other With respect to the FE models, the analytical model predicts a very stiff stability ... loads The run time of an FE analysis ranged from 45 minutes up to 60 minutes The run times of the MATLAB implementation of the analytical model were less than 10 seconds Results The progress of the ... assumptions of the analytical model as good as possible The stems were chosen in such a way that they span a wide range of antirotation values, based on the analytical model STL-files of the stem...

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