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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 7

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 7

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 7

... amino acids in their core Being anionic, SDS has a negative charge over a wide pH range Therefore, mixing the proteins in a biological sample with SDS will result in proteins having the form of ... the stacking gel, causing the proteins to be compressed and ‘stacked’ into a tight band, less than mm thick, at the running front of glycine before it reaches the resolving gel Once the running ... silver ions bind to the proteins in the gel, and are reduced to metallic silver, causing the protein bands in the gel to be visualized [21] This method forms the basis of the Silver Stain Plus kit...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 1

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 1

... directly into the environment [13 , 14 ] The major routes by which the aforementioned environmental toxicants enter the body are through the skin, the lungs, and the gastrointestinal tract and they ... occurs in toxic environment 11 Chapter containing one or more carcinogens [ 31, 32] Hence, the information on the body burden of carcinogens provides evidence for malignant transformation 1. 7 Importance ... understanding of the origin and progress of a disease For detection and quantification of xenobiotics in complex biological samples, many sophisticated laboratory techniques, developed during the...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 2

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 2

... [5-8] Therefore, determining the level of estrogens in these body fluids would be very important for the study of their roles in the carcinogenesis of ovarian and breast cancer The role of estrogen ... 300◦C and the interface temperature kept at 28 0◦C The GC temperature program used was as follows: initial temperature 90◦C held for min, then increased by 30◦Cmin-1 to 28 0◦C, and held for The standard ... width of ~2 cm The edge of the fold-over flap was then heat sealed using an electrical sealer to the main sheet The fold-over section was then trimmed off from the main membrane sheet The former...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 3

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 3

... present in those samples In a biplot, the length of the lines approximates the variances of the variables The longer the line, the higher is the variance Inferring from Figure 3. 7, 3- nitroaniline ... depends on the axes and the points As for the points, the `quality' of the PCA approximation is found from the R program In this case, the quality 0.627 implies that 62.7% of the variation in the samples ... was maintained at a constant flow of 0.5mL min−1 Injection volumes of 3mL were used The injection port was held at 230 ˚C and used in the splitless mode, applying a pressure pulse of 40 psi The...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 4

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 4

... 310˚C min-1 and 5˚C min-1 to 320˚C (hold for 10 min) µL of the sample was injected into the GC-MS in splitless mode and the total GC-MS analysis time was 55.00 SIM mode employed for the set of target ... present in 89% of both samples Of the 87 congeners detected congeners (CBs 18, 31, 44 , 66, 149 , 1 74, 180, 1 94 and 203) were present in more than 20% of the samples The results indicated that there ... Measurements of the PCBs or their metabolites in body tissues and fluids (often called biological monitoring) have been carried out as useful approach for assessing the exposure risk in the epidemiological...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 5

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 5

... research for the determination of trace metal status of individuals and groups [9-11] Nonetheless, in the case of ovarian tumor, both benign and malignant ovarian cyst fluids are good source of information ... has increased in the recent decade, not only the trace metals but the heavy metals as well The study of the role of the trace elements has been emphasized in the search for the possible causes of ... determined in body fluids and tissues for the diagnosis and monitoring of various disorders, nutritional deficiencies, and occupational or environmental exposure [1-3] The number of elements of interest...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 6

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 6

... withdrawn into the GC syringe and the needle of the syringe was inserted into hollow fibre to about 0.2 cm of the tip of the syringe needle was covered by it The hollow fibre was then dipped into the ... Apopka, FL, USA) in the morning and in the evening Fish were maintained on a photoperiod of 16 h light:8 h dark The pH ranged from 7.0 to 7 .6 throughout the duration of the experiment, and ammonia ... discarded) Using the same syringe, µL of BSTFA was withdrawn, making the total volume in the GC syringe µL The entire µL was injected into the GC-MS for injection port derivatiztion and analysis...
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Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 8

Bioanalytical strategies for the quantification of xenobiotics in biological fluids and tissues 8

... plasma and serum, ovarian cyst fluids are more relevant to the objective of the study Further, the cyst fluids were studied for their and inorganic trace element levels by applying a combination of ... temperature) and derivatization of analytes were optimized for better identification Since the aim of the study was to explore the biologically important tumor fluids and tissues, the choice of analytes ... Considering other xenobiotics, the levels of each compound was quantified in benign and malignant of cyst fluids The results showed that, although, most of the compounds were present in both groups of...
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Báo cáo y học:

Báo cáo y học: " A multi-center, randomized, double-blind, parallel, placebo-controlled trial to evaluate the efficacy, safety, and pharmacokinetics of intravenous ibuprofen for the treatment of fever in critically ill and non-critically ill adults" pdf

... article as: Morris et al., A multi-center, randomized, double-blind, parallel, placebo-controlled trial to evaluate the efficacy, safety, and pharmacokinetics of intravenous ibuprofen for the treatment ... demonstrates baseline data There were no differences among the four arms of the study in baseline demographics Also, analyses of baseline data compared critically ill and non -critically ill populations ... Materials and methods This multicenter, randomized, double-blind, parallel, placebo-controlled clinical study was designed to assess the efficacy, safety, and pharmacokinetics of IV ibuprofen in adult...
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Báo cáo y học:

Báo cáo y học: " Updating the evidence for the role of corticosteroids in severe sepsis and septic shock: a Bayesian meta-analytic perspective" pot

... Yes Yes Yes (44%) NA Yes (93%) NA NA NA Vasopressors atenrollment Yes Yes NA Yes NA NA NA NA NA NA NA NA NA NA Etomidate used Bolus-day1 Steroid-days before tapering Bolus-day1 Hydrocortisone Hydrocortisone ... colleagues Annane and Table Trial patient data by outcome (Continued) #, mortality statistics for Chawla and colleagues [47] were abstracted from the Annane and colleagues meta-analysis[6] ##, data ... Glucocorticoid action on inflammation [57], vascular reactivity [58] and interactions between corticosteroids and ‘signalling pathways’ [59] may explain the salutary effects in sepsis [60]; anti-inflammatory...
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Báo cáo y học:

Báo cáo y học: "Evaluation of an ambulatory system for the quantification of cough frequency in patients with chronic obstructive pulmonary disease" ppsx

... decubitis; Cough = cough output from algorithm The shaded bar contains the cough event Cough is indicated by a single solid line at the end of the breath that contains the cough Note the change in the ... breathing frequency (median Fb = 21 br·min-1) The system was highly accurate in identifying cough as a respiratory event during night or day Accuracy during the night was 99.4%, while accuracy during ... evaluation of antitussive therapies The availability of a valid, robust ambulatory tool for quantifying cough will enable the determination of the minimal required reduction in cough to maximally improve...
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Strategies for the conservation of two critically endangered, endemic primates in panama

Strategies for the conservation of two critically endangered, endemic primates in panama

... with information on the three subspecies of Azuero primates, indicating how they can help to minimize the risk of their extinction The initiative also gives away t-shirts that have images of the ... and the presence of primates and other mammals situation of the monkeys in the area and one regarding the vocalization behavior of the Azuero howlers Future plans include engaging the Panama Ministry ... obtain information about the history of the fauna, native plant knowledge, and the presence and problems related to the primates Training students from the Biology School of the University of Panama...
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Báo cáo y học:

Báo cáo y học: "Thioglycosides as inhibitors of hSGLT1 and hSGLT2: Potential therapeutic agents for the control of hyperglycemia in diabetes"

... orally [27] Therefore, the aim of the present study was to evaluate the inhibitory effect of some thioglycosides synthesized in our laboratory on human hSGLT1 and hSGLT2 as a potential therapeutic ... O-glucosides involved in the biosynthesis of chrysomelidial and salicin These substances are synthesized and secreted as part of a defense mechanism used by larvae of beetles (Chrysomelidae) Their synthesis ... synthesis has been previously described [31-33] For the purpose of the present study the thioglycosides used were selected and grouped based on their differences in the aglycone binding site or in the...
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Tài liệu OUTLINES OF DAIRY BACTERIOLOGY A CONCISE MANUAL FOR THE USE OF STUDENTS IN DAIRYING docx

Tài liệu OUTLINES OF DAIRY BACTERIOLOGY A CONCISE MANUAL FOR THE USE OF STUDENTS IN DAIRYING docx

... faculty of growing either as parasites or saprophytes, in which case they are known as facultative parasites or saprophytes The great majority of bacteria of interest in dairying belong to the ... together with a limited amount of mineral matter The nitrogen and carbon are most available in the form of organic compounds, such as albuminous material Carbon in the form of carbohydrates, as ... differentiated, and that is, that practically all of them are capable of producing their characteristic chemical transformations under anaesthetic conditions, as in a saturated ether or chloroform atmosphere...
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Tài liệu Guideline on Best Practice for the Audit of Risk in Public/Private Partnership (PPP) docx

Tài liệu Guideline on Best Practice for the Audit of Risk in Public/Private Partnership (PPP) docx

... aspects of the privatisation process: • guidelines on best practice for the audit of privatisations (adopted by XVI INCOSAI in 1998) • guidelines on best practice for the audit of economic regulation ... XVII INCOSAI in 2001); and • guidelines on best practice for the audit of public/private finance and concessions (also adopted by XVII INCOSAI in 2001) The Working Group’s previous guidelines ... Identifying worthwhile lessons, continued Risk Managing the risk 34) The SAI may build a model of good • practice in procuring and monitoring partnerships In applying this to the deals it is examining,...
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