Application of recombinant antibody technology for the development of anti lipid antibodies for tuberculosis diagnosis

... APPLICATION OF RECOMBINANT ANTIBODY TECHNOLOGY FOR THE DEVELOPMENT OF ANTI- LIPID ANTIBODIES FOR TUBERCULOSIS DIAGNOSIS CONRAD CHAN EN ZUO BSc (Hons.), MRes Imperial College London A THESIS ... development of monoclonal hybridomas, it required refolding of the antibody from the insoluble protein fraction (121) Instrumental for the expression of soluble antibody fragments was the development of ... display allows for the selection of such antibodies in vitro without a requirement for an immune response (10,11) We have therefore explored antibody phage display for the generation of high affinity,...

Báo cáo hóa học: "Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation" pot

... Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation Journal of Translational Medicine 2010 8:137 Submit your next manuscript to BioMed Central and take full advantage of: • ... within samples For example, goat pAb reacted weakly with only one form of gelsolin in the CSF whereas rabbit pAb recognized strongly two forms of gelsolin in that same sample None of these antibodies ... in plasma/CSF Therefore, neither of these two peptides will reflect accurately levels of pGSN; additionally, use of these peptides in MRM may not validate pGSN as potential biomarker explained,...

Báo cáo y học: "α The role of anti-α-actinin antibodies in the pathogenesis and monitoring of lupus nephritis" pot

... strongly to α-actinin [12,13] Moreover, an increase in serum anti-α-actinin antibodies in lupus patients was associated with a 2.5-fold increase in the prevalence of LN [14] Finally, levels of anti-α-actinin ... followed by Manson and colleagues [1] were being treated, and anti-α-actinin antibody titers may decrease with therapy Finally, perhaps anti-dsDNA antibodies not measured by the assay used in this ... candidate in murine lupus Furthermore, high titers of anti-α-actinin antibodies were present in the serum and kidney eluates of LN mice [8] Our results confirmed and extended those reported by Mostoslavsky...

Báo cáo y học: "Immediate determination of ACPA and rheumatoid factor - a novel point of care test for detection of anti-MCV antibodies and rheumatoid factor using a lateral-flow immunoassay" pdf

... in both standardized anti-MCV and RF-ELISA (Orgentec, Mainz, Germany) The ratio of applied antigens and serum anti-MCV antibodies and/ or RF was such that monodentate binding of autoantibodies ... the anti-CCP2 ELISA Scand J Rheumatol 2008, 37:15 1-1 54 doi: 10.1186/ar3057 Cite this article as: Renger et al., Immediate determination of ACPA and rheumatoid factor - a novel point of care test ... simultaneous detection of anti-MCV antibodies and RF-IgG using POCT As a major technical difference to established immunoassays, this POCT is based on the ability to detect anti-MCV antibodies and...

IMMOBILIZATIONS OF ANTI JEV ANTIBODIES IN HUMAN SERUM FOR DIRECT IMMUNOSENSORS

... CLEANING PROCEDURES sensors 3-aminopropyl-triethoxy-silance (APTES) Experimetal immobilizations of anti JEV serum APTES – Antibody - Immobilization with mg/ml anti- JEV antibodies contained human serum ... solution for 30 - Immobilization with mg/ml anti- JEV antibodies contained human serum for hour -Blocked with 2% BSA solution in PBS for 30 minutes - Washing with phosphate buffered saline (PBS) ... electrodes area for 30 - Immobilization with mg/ml anti- JEV antibodies contained human serum for hour - Blocked with 2% BSA solution in PBS for 30 minutes Washing with phosphate buffered saline (PBS)...

DNA chip platform a high throughput genotyping technology for genetic diagnosis and pharmacogenetic profiling

... relapse In conclusion, DNA chip platform is a high- throughput and reliable technology for genetic diagnosis and pharmacogenetic profiling ix LIST OF TABLES Page Table 1.1 Features of traditional ... severe anaemia, in between that of thalassaemia minor and major, afflicts the patient This is termed thalassaemia intermedia Southeast Asia lies in the malaria belt where for thousands of years, malaria ... DNA chip- based genotyping platform to obtain pharmacogenetic profiles of children with acute lymphoblastic leukaemia 1.2 Pharmacogenetic analyses in childhood acute lymphoblastic leukaemia (ALL)...

Báo cáo y học: "Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies" ppsx

... (Rheumaklinik Aachen, Aachen, Germany), Prof Dr MJ Fritzler (University of Calgary, Calgary, Canada) and by Labor Limbach (Heidelberg, Germany) To assess further the assay specificity, we analyzed ... studies are necessary to screen known autoantigens containing dimethylated arginine residues for epitopes assay Assay performance characteristics of the anti -SmD3 peptide (SMP) assay (a) Intra-assay ... Intra-assay and interassay variability, (b) linearity, and (c) receiver operating characteristic analysis The intra-assay and interassay variability, expressed as coefficient of variation in percentage...

Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx

... biomarkers, and they participated in the data analyses AAH developed the customized Spotfire tool used for data analyses and reviewed statistical analyses YG participated in the assessment and ... assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood Journal of Translational Medicine 2010, 8:51 Page 13 of 13 ... this basic approach (ex vivo stimulation of whole blood) to develop pharmacodynamic biomarker assays for a candidate therapeutic antibody, Ab-01 Ab-01, a human antibody generated by phage display,...

Báo cáo y học: " Development and evaluation of an immunochromatographic strip test based on the recombinant UL51 protein for detecting antibody against duck enteritis virus" ppsx

... faster than the time required for the ELISA and NT assays, and the results can be read directly by the naked eye Therefore, the ICS test is a high specific and sensitive assay for the rapid and reproducible ... require any equipment or skilled technicians and can be conveniently performed on the duck farm by a duck farmer Importantly, the detection of DEV-specific antibodies by the ICS test only takes ... with the gold standard assay, NT, the sensitivity of the ICS test was significantly higher than the NT Unlike these commonly used assays, the ICS test for the detection of DEV-specific antibodies...

DEVELOPMENT OF THE OZONIZER AND OZONATION TECHNOLOGY FOR WATERWORKS IN JAPAN

... In Raw Water On The Ozonation Of Geosmin And 2-MIB OZONE SCIENCE & ENGINEERING 15, 1-18 Morioka, T (2001) The Study on the Application of Ozonation for the Removal of the Taste and Odor-Causing ... concentration and decomposition RESEARCH AND DEVELOPMENT IN OZONATION TECHNOLOGY Objectives of ozonation The main objectives of ozonation are to reduce trihalomethane and to remove taste and odor These ... mg.min/L , and in the range of 10 to 12 mg.min/L, is required These values can be classed as practical Technique for suppressing bromate formation The ozonation of raw water containing bromide...

Tài liệu Báo cáo khoa học: Application of a ﬂuorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf

... Expression and purication of human recombinant IF and TC The recombinant Cbl binding proteins and their fragments were isolated from plants and yeast as described earlier [9,17] Preparation of the unsaturated ... Fedosova NU, Nexo E & Petersen TE (2002) Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, transcobalamin, and haptocorrin J Biol Chem 277, 99899996 Cannon ... uorophore, as was expected from the crystallographic data for TCCbl complex [16], and the binding- dissociation curves of CBC and Cbl were identical (Figs 2B,4B Application of a uorescent Cbl analogue and...

the development and application of an antibody based biosensor

... spectrum of BT5AA 56 Synthesis and mass spectrum of 2TAA 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of ... from the ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proportions of ... Schematic of a generalized biosensor The bioreceptor and the transducer are linked such that the transducer quantifies the biorecognition event occurring between the sample and the bioreceptor The...

Báo cáo hóa học: " Combination of Polymer Technology and Carbon Nanotube Array for the Development of an Effective Drug Delivery System at Cellular Leve" pptx

... concentration on the surface of the film and finally ks is the desorption rate constant Substituting known values and by fitting the experimental data with the mathematical model of Eq 1, the h value ... bulk, C10 is the concentration inside the gel, S and V1 are, respectively, the surface and the volume of film, V2 is the volume of the bulk, h is the massive exchange coefficient, Cs0 is the protein ... illustration of the proposed CNT-based system Nanoscale Res Lett (2009) 4:668–673 671 Fig Bare (a), and SiO2coated (b) CNT array (samples dipped in water and dried in air before the imaging) 120 % of...

báo cáo khoa học:" Study on the clinical application of pulsed DC magnetic technology for tracking of intraoperative head motion during frameless stereotaxy" pdf

... 2:10 http://www .head- face-med.com/content/2/1/10 Figure1 Type B fixation of the head Type B1 fixation of the head (A) Patient positioned on the right side for resection of a left frontal metastasis ... chosen on the basis of the image data Accuracy of frameless stereotaxy with the DRF in the clinical application The overall precision of the DRF in clinical use is affected by various factors These ... all other procedures, continuous tracking of head motion ensures automatic correction of spatial distortion with mechanical alteration of the head position With a special dental cast for the oral...

Báo cáo y học: " Risk factors for the development of nosocomial pneumonia and mortality on intensive care units: application of competing risks models" ppt

... waived the need for informed consent Further details on the setting of the study are described elsewhere [9,10] Analysis of risk factors for the acquisition of NP (model 1) In model 1, we studied risk ... the ICU Overall, in 214 of the 1,876 admissions (11.4%) the patient died in the ICU More details of risk factors and outcomes are shown in Table Analysis of risk factors for the acquisition of ... of risk factors for mortality (model 2) Detailed information on the CSHRs of baseline and timedependent risk factors of model are shown in Table The baseline variables of age, SAPS II and other...

Xem thêm

Từ khóa: xw gu zm wang bx hattori m namba t 1991 quot comparison of anti lipid peroxidative effects of the underground parts of notopterygium incisum and n forbesii in mice quot planta med 57 5 pp 399 402 fine specificity of anti cd4 antibodies in hiv 1 exposed individuals with different susceptibility to hiv infection detection of anti lactobacillus antibodies along with the development and upgrade of hybrid rice technology the market for hybrid rice seed increased 111 fold from 0 14 million ha in 1976 to 15 4 million ha in 2000 hybrid rice would not have been succes science and technology for development the new paradigm of ict the application of transgenic mice for therapeutic antibody discovery stimuli sensitive polymer gels for dermal and transdermal drug delivery and their application in the development of smart textile materials rationale for application of current technology the role of neural plasticity review of experience and evidence for the application of specific technology the discovery that micrornas mirnas are synthesized as hairpincontaining precursors and share many features has stimulated the development of several computational approaches for identifying new mirna genes in various animal species and there is an immediate need for the development of novel and more effective therapeutic modalities against this deadly disease architecture for the development of contextsensitive mobile applications the development of the internal combustion engine was responsible for the role of information and communication technology in agricultural development the evolution of screw design technology for the injection molding process Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt nam Nghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấp Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTP Biện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọ Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit ĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN ĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN Quản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninh Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùng Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chế Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tin Thơ nôm tứ tuyệt trào phúng hồ xuân hương Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIX Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2 Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015 Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam