Structural and binding characterization of the antiviral host proteins, VIPERIN and VAPC

... RESULTS AND DISCUSSION 2.3 Structure details of Viperin 2.3.1 Structural characterization of the human viperin fragments 42-45 2.3.2 Reconstitution of the [4Fe–4S] cluster in Viperin (45–361) and viperin ... 3.3.4.1 Structural characterization of VAPC4 3, VAPC3 1 and VAPC1 4 90-93 3.3.4.2 Interaction of VAPC4 3 with HCV NS5B 94-98 3.3.3.3 Interaction of VAPC1 4 with HCV NS5B 98-99 3.3.4 Determination of dissociation ... The signals of the amides which get perturbed by ligand binding will change position, on the addition of ligand The perturbed residues can be mapped upon the protein structure to reveal the binding...

Báo cáo khoa học: Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms docx

... Mukaiyama et al B BSA holo-NPAS2 ∆ F (Hz) holo-NPAS2 holo-NPAS2 holo-NPAS2 holo-NPAS2 a p o- N PAS apo-NPAS2 apo-NPAS2 apo-NPAS2 BSA apo-NPAS2 ∆ F (Hz) A Characterization of bHLH -PAS- A of NPAS2 T ... (bHLH) -PAS- A domain FEBS Journal 27 3 (20 06) 25 28 25 39 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Mukaiyama et al Characterization of bHLH -PAS- A of NPAS2 Table Resonance Raman spectra of the ... of NPAS2 Y Mukaiyama et al Table Resonance Raman spectra of Fe(II)–CO complexes of the basic helix–loop–helix (bHLH) -PAS- A and PAS- A domains of neuronal PAS domain protein bHLH -PAS- A PAS- A mC–O...

Báo cáo khoa học: Characterization of the 16S rRNA- and membrane-binding domains of Streptococcus pneumoniae Era GTPase Structural and functional implications docx

... Effects of deletion of the GTPase domain on the 16S rRNA-binding activity of Era Results Effects of the deletion of the RNA- and membranebinding domains of S pneumoniae Era on the GTPase activity of ... growth Analysis of the association of the truncated Era proteins of S pneumoniae with the cytoplasmic membrane To examine the association of Era, Era- N, and Era- C of S pneumoniae with the cytoplasmic ... not clear whether the binding of Era to 16S rRNA requires the GTPase activity of the protein and whether the RNA-binding domain can bind RNA in the absence of the GTPase domain Structurally,...

Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx

... 1169–1178 ª 2005 FEBS Characterization of Drosophila Met gene product of pBluescript II (Stratagene), and the identity of the cDNA clone was conﬁrmed by sequencing Binding assay The DCC assay was ... sequence Therefore, it is reasonable to consider that the GAL4–DBD fusion of MET sits on the UAS of the reporter construct even in the absence of ligand, and that the MET moiety is responsible for the ... of Drosophila Met gene product exhibited speciﬁc ligand-dependent activation of a reporter gene placed downstream of the UAS The MET fusion responded only to JH or the JHA methoprene while compounds...

Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... for the Ndh membrane subcomplex The Ndh antibodies recognized the 46, 28, 18 and 12 kDa polypeptides, corresponding to the NdhH, -K, -J and -E subunits of the Ndh complex The intact Ndh complex, ... et al of the ndh genes is much higher in BS chloroplasts, and elevated amounts of the Ndh complex have been found in these plastids [18] The function of the Ndh complex is still a matter of debate ... kDa), NdhE (12 kDa), NdhF (83 kDa), NdhG (18 kDa), NdhH (45 kDa), NdhI (21 kDa), NdhJ (18 kDa), NdhK (29 kDa) The 75, 51 and 23 kDa subunits are assigned as the soluble subcomplex of the Ndh complex...

Báo cáo khoa học: "Structural and functional characterization of the 5’ upstream region of a glutamine synthetase gene from Scots pine" pdf

... in all compared organisms We have also analyzed the presence of putative elements in the 5’ region of the gene There is a canonical TATA box at –35 bp from the transcription start site and a putative ... were used as controls 2.4 Gel retardation analysis A DNA fragment used for gel retardation analysis containing a sequence from the 5’- untranslated region of GS 1a was obtained by cleavage with ... with the reporter gene uidA and the presence of DNA-protein interactions in the 5’flanking region of GS 1a gene from Scots pine MATERIALS AND METHODS 2.1 Isolation of a genomic clone containing...

Báo cáo y học: "Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase" ppt

... chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase Virology Journal 2010 7:68 Submit your next manuscript to BioMed Central and take full ... sciences, Maryland, USA) following the manufacturer’s instruction The reverse transcription activity of HIV-1 in the infected cells was analyzed by quantifying the total HIV cDNA by using the qPCR ... antibody B) The intensity of both the chromatin-bound and non-chromatin-bound YFP-IN was densitometrically determined The data are presented as the percentage of chromatin-bound YFP-IN to total...

Characterization of the Carbon Stable Isotope Ratio and Fatty Acid Structure of Zostera japonica in Coastal Areas

... acid structure in Z japonica We found about 30 kinds of fatty acids in the leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid ... based on the quantity of lipid, the content ratios of all the fatty acids, and the ratio of each fatty acid RESULTS AND DISCUSSION Characteristics of carbon stable isotope in Z japonica The mean ... dominant fatty acids and Fig shows the dominant fatty acids in the leaves and stems of Z japonica in each sampling site The term “other” in the x-axis designates the sum of all the fatty acids with...

Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells

... PVDF-SPS composite membrane Table shows the water uptake of the pristine PVDF membrane, the PVDF-PS membrane and the PVDF-SPS composite membrane The blending of PS polymer reduces the water uptake of ... of the PVDF-SPS membrane: (a) C1s, (b) O1s, (c) S2p 3.4 Morphology of the PVDF-SPS composite membrane The morphologies of the pristine PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane ... polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the PVDF membrane, the PVDF-PS membrane...

$Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx$

Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx

... Organic Process Research & Development 2007, 11, 846–860 Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique David ... sensors of the DSC cell Thermal isolation was maintained by using beryllium metal foil to seal the X-ray optical path identiﬁcation and understanding of the thermal behavior of the various polymorphic ... melt and creates an uncertain baseline Second, the varying quantities of water in the starting material (Form I) result Table Summary of DSC data, suggested results, and additional characterizations...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin ﬁsh [28] have shown similar widespread expression In the case of STAT6, highest...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... number of Arabidopsis transcripts are potential targets for regulation by the PUF family of proteins The results obtained reveal a molecular conservation of PUF proteins in Arabidopsis thaliana and ... regarding the binding speciﬁcity of the subset of group I APUM proteins, showing that A thaliana has at least six PUF proteins with conserved RNA -binding and similar speciﬁcity The group I APUM proteins ... analysis of plant proteins possessing PUF repeats Using computational analyses and yeast three-hybrid assays, we found that at least six Arabidopsis thaliana proteins PUF proteins in Arabidopsis...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the ﬁndings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III proteins The ... strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure protruding from...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... (2002) A novel interaction partner for the C-terminus of Arabidopsis thaliana plasma membrane H+-ATPase (AHA1 isoform): site and mechanism of action on H+-ATPase activity differ from those of 14-3-3 ... Rasi-Caldogno F (1998) Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H+-ATPase IV Fusicoccin induces the association between the plasma membrane H+-ATPase ... representative of three giving similar results 5865 Interaction between plasma membrane H+-ATPase and PPI1 Fig Stimulation of A thaliana PM H+-ATPase activity as a function of the concentration of...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with a His-tag and Snz1p ... SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clariﬁed Expression and puriﬁcation of Sno1p and Snz1p In light of the similarity in the amino acid ... Sequencing of the mutated genes revealed that there was indeed a mutation on both of the genes In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift...

Xem thêm