Structural and epitope characterization of major allergens from dust mite, BLO t 21 and DER f 7

... www.pdffactory.com List of Tables IgE binding rates of the cloned dust mite allergens and their biological properties 22 Overview of the productions of recombinant mite allergens: from the aspects of the production ... mutants of the mites FABP homologues 70 Overview of the characteristics and statistical data of the dust mites EST collections 80 Summary and comparisons of gene expression levels in D farinae and ... House dust mites as an important cause of allergy 1.6.1 General aspects of mites Mites are normal inhabitants in our environment and are abundant in house dust as well as barns and grain stores Mites...

... STRUCTURAL AND EPITOPE CHARACTERIZATION OF DUST MITES ALLERGENS, DER F 13 AND BLO T CHAN SIEW LEONG (B Sc., USM) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... 3.1 Expression and purification of His-tag Der f 13 79 Figure 3.2 Expression and purification of GST-tag Der f 13 79 Figure 3.3 Gel filtration profile and CD spectrum of Der f 13 80 Figure 3.4 One ... of Der f 13 83 Figure 3 .5 84 Figure 3.6 Sequential assignment of backbone chemical shifts of Der f 13 85 Figure 3.7 Side chain 13C chemical shifts 86 Figure 3.8 Splitting pattern of different...

... mite allergens 2.3 Domestic mites Domestic mites consist of various free-living mites that are found living in houses This includes house dust mites (HDM) (family Pyroglyphidae) and storage mites ... 56 v Cloning of a unique allergen from Blomia tropicalis and monoclonal antibody production 58 4.1 Objectives and significance of the study 58 4.2 Materials and methods ... Sensitization profiles of rhinitis, non-rhinitis healthy subjects and asthmatic subjects (from Singapore and Malaysia respectively) against three major mite allergens Der p 1, Der p and Blo t were...

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 2003 Laccase gene from Volvariella ... min; then a final extension at 72 °C for 10 The primers for lac1 PLAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢) and PLAC1R (5¢-AACGAG CTCAAGTACAAATGACT-3¢) were designed according to our cloned cDNA (GenBank...

... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O-glycan pool was analysed by MALDI-TOF ... N-glycans Ó FEBS 2002 Novel kininogens from cod and wolffish (Eur J Biochem 269) 2643 Fig Alignment of peptides from cod kininogen (COD KIN) and wolf fish kininogen (WF KIN) with heavy chain of human...

... complex from carbonatewashed membranes of SY5Y and 5-day-old mouse brain were examined using 10% Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex ... assembly and stabilization of the complex [50–52] High levels of PS complex were detected in mouse embryonic brain consistent with high expression of PS1 in embryogenesis and supporting an important ... analysis from mouse brain by BN/PAGE indicated that solubilization of membranes with 0.5% DDM Ó FEBS 2003 Analysis of mouse and human brain presenilin complex (Eur J Biochem 271) 377 Fig BN/PAGE of...

... understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion Cloning and expression of Lc lactis prolidase The prolidase ... protein engineering of this enzyme In the present study, the prolidase- coding gene, pepQ, was isolated from Lactococcus lactis NRRL B-1821 and cloned Characterization of the recombinant protein revealed ... Tanaka Recombinant Lactococcus lactis prolidase Under these conditions, 40 h cultures produced 2 0– 40% of soluble proteins as recombinant prolidase, as determined by densitometry of the SDS–PAGE...

... similar to that of curdlan, indicating that it is a b-linked polysaccharide (Fig 5) Fig Isolation and purification of polysaccharides extracted from cultured mycelia of Cordyceps militaris (A) Ion ... methylated alditol acetates of the purified polysaccharide, CPMN Fr III, isolated from cultured mycelia of Cordyceps militaris Polysaccharide Alditol acetate derivative Type of linkage Relative molar ... composition and monosaccharide composition of purified polysaccharide, CPMN Fr III, from cultured mycelia of Cordyceps militaris (%, dry basis) Polysaccharide Protein Hexosamine Uronic acid Total...

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

... al.: In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs Radiation Oncology 2011 6:116 Page of Submit ... subpopulation of U-CH1, chromosome and p53 analysis U-CH1- N cells were verified for their characteristics on karyotyping compared with their original U-CH1 cells Page of U-CH1- N cells were cultured with ... sacral chordoma cell line Methods Cell lines and culture conditions The chordoma cell line U-CH1 was kindly supplied by the Chordoma Foundation in Greensboro, NC, USA U87-MG and HeLa cell lines...

... flavonol-O-glucosyltransferases from Fragaria × ananassa (AAU09444) and (ABB92748); NtGT 1a and NtGT1b, broad substrate specificity glucosyltransferases from Nicotiana tabacum (BAB60720) and (BAB60721); ... Ubukata T, Hayashida N, Yamamoto H, Okazaki M: Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco ... 3-O-glucosyltransferases from Arabidopsis thaliana (AAD17392), AtUGT73B4 and (At5G17050), At GT; Zea mays (X13502), ZmF3GT; from Vitis vinifera (AAB81682), VvF3GT; from Fragaria × ananassa (BAA12737),...

... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Figure 4.1: Synthetic gene sequences of Fasxiator Figure 4.2: Identification of novel anticoagulants from Bungarus fasciatus venom Figure 4.3: Effects of BF01 and BF02 on various procoagulant proteases ... ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure 3.3: Structural characterization of BF-AC1 and BF-AC2 XI Figure 3.4: N-terminal sequence alignment of Chain A and Chain B of BF-AC1 and BF-AC2...