Identification and characterization of novel anticoagulants from bungarus fasciatus venom

... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Figure 4.1: Synthetic gene sequences of Fasxiator Figure 4.2: Identification of novel anticoagulants from Bungarus fasciatus venom Figure 4.3: Effects of BF01 and BF02 on various procoagulant proteases ... ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure 3.3: Structural characterization of BF-AC1 and BF-AC2 XI Figure 3.4: N-terminal sequence alignment of Chain A and Chain B of BF-AC1 and BF-AC2...

Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides

... Bibliography 195 Appendix 224 Publications 238 vi Summary Identification and characterization of novel proteins from a rare Australian elapid snake Drysdalia coronoides Partial transcriptome from ... venomous terrestrial snakes of the Elapidae family have undergone an extensive radiation in Australia [12] Elapid snake genus Drysdalia from southern Australia is a group of rare snakes comprising ... islands of Ireland, Iceland and New Zealand [5, 6] They have successfully colonized various habitats and feed on small animals including lizards, snakes, rodents, small mammals, birds, eggs and...

Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5

... thermal treatment on IgE- binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE- binding of Blo t and Blo t 21 to acid, ... 21 75 and Blo t 3.2.10 Quantitative end point cross-inhibition of Blo t 21 and 77 Blo t 3.2.11 Localization of Blo t 21 and Blo t on sections of mite 79 body 3.2.12 3.3 Concentration of Blo t ... important allergen of B tropicalis The IgE- binding activities of both Blo t and Blo t 21 are stable Blo t 21 and Blo t are resistant to heat treatment (up to 90 °C), extreme pH conditions (pH and...

Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

... N-Terminal sequencing of the kininogens was only successful from the 42-kDa band of wolffish kininogen (XLVQPGVLI…, Table 1) The major bands of both kininogens and also the 60-kDa band of cod kininogen ... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O-glycan pool was analysed by MALDI-TOF ... N-glycans Ó FEBS 2002 Novel kininogens from cod and wolffish (Eur J Biochem 269) 2643 Fig Alignment of peptides from cod kininogen (COD KIN) and wolf fish kininogen (WF KIN) with heavy chain of human...

Characterization of novel anticoagulants from hematophagous arthropods

... pillar of support iv TABLE OF CONTENTS ACKNOWLEDGMENTS i TABLE OF CONTENTS v Summary viii List of Tables x List of Figures xii List of Abbreviations ... classification of extracted coding sequences (CDS) from the putative housekeeping class from the sialotranscriptome of R pulchellus 66 Table 9: Functional classification of proteins from the ... housekeeping class from the proteome of R pulchellus 67 Table 10 Functional classification of extracted coding sequences (CDS) from the putative secreted class from the sialotranscriptome of adult Rhipicephalus...

Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... Effect of KCl on the activity of salt-active acharan sulfate lyase d, salt-active acharan sulfate lyase II purified from Fr-s in Fig 1; m, heparin lyase II-1 (acharan sulfate lyase 1) purified from ... specificity of purified salt-active acharan sulfate lyase The purified salt-active acharan sulfate lyase degraded heparin and heparan sulfate as well as acharan sulfate (Table 5) The salt-active acharan sulfate ... Results Purification of salt-active acharan sulfate lyase Bacteroides stercoris HJ-15, which degrades a variety of GAGs including heparin, heparan sulfate, acharan sulfate and chondroitin sulfates...

Báo cáo khoa học: Identiﬁcation and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc

... activation of factors V and VIII by thrombin and then prevent these cofactors from forming prothrombinase and tenase complexes On the other hand, as shown in Fig 7, madanin and suppressed thrombin- induced ... salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged both APTT and PT, suggesting the presence of an inhibitor of either factor Xa or thrombin ... measured using the coagulometer Assay of effects of madanin and on activation of factor V and factor VIII by thrombin The effect of the madanins on the activation of factor V by thrombin was determined...

Báo cáo Y học: Puriﬁcation and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx

... puriﬁcation and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from an anaerobic bacterium (B stercoris) in human intestine The substrate speciﬁcity and other ... suggest that chondroitin ABC and AC lyases have exolytic and endolytic action patterns These chondroitin ABC and AC lyases showed optimal activity at pH 7.0 and 5.7–6.0 respectively, like most of previously ... activity (%) F heparinuma B stercoris B thetaiotaomicrona P vulgarisa Substrate ABC AC ABC AC ABC ABC Chondroitin sulfate A Chondroitin sulfate C Dermatan sulfate Hyaluronic acid Heparin Heparan...

Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot

... doi:10.1186/2191-0855-1-26 Cite this article as: Phan et al.: Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus AMB Express 2011 1:26 ... such as KM, Vmax, and Kcat Applicability of P excavatus proteases Serine proteases The inhibitory effect of PMSF towards all fractions revealed that P excavatus proteases are serine proteases, ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx

... al.: Identification and characterization of alkaline serine protease from goat skin surface metagenome AMB Express 2011 1:3 Submit your manuscript to a journal and beneﬁt from: Convenient online ... and Gly-Thr-SerMet-Ala-X-Pro, which is characteristic of serine subfamily S8A Results from the sequence analysis of this protease suggested it to be serine protease subfamily S8A Expression of ... stearothermophilus protease promoter Protease promoter represents the predicted alkaline serine protease promoter region E coli protease promoter represents, E.coli lon protease promoter Effect of pH and temperature...

Báo cáo y học: "Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet''''s disease" docx

... the protein Sip1 Sip1 is a nuclear splicing factor containing an arginine/serine-rich domain and a RNAbinding motif that may play a role in linking the processes of transcription and pre-mRNA ... participated in the design and revision of the study and performed the statistical analysis RP participated in the design and revision of the study AS participated in the analysis and interpretation ... of data and helped to draft the manuscript RR participated in the design of the study and in the revision of the manuscript EP participated in analysis of data GV participated in the design of...

Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx

... staining (g) Intense staining of a hyperplastic RA synovial lining cell layer This staining was typical of most areas of RA synovium where the lining was hyperplastic (h) An area of an RA synovial ... stained positively (arrow) (e) Intense staining of individual cells in the lining layer of a typical OA synovium (f) An area of an OA synovium demonstrates a lining layer completely devoid of ... synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular regions of OA (paraffin section) tissue (c) An area demonstrating...

Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction

... overall incidence was found in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique ... IDENTIFICATION AND CHARACTERIZATION OF A NOVEL HEART- REACTIVE AUTOANTIBODY IN SYSTEMIC LUPUS ERYTHEMATOSUS: POSSIBLE SEROLOGICAL MARKER FOR EARLY MYOCARDIAL DYSFUNCTION XU QIAN (M Med., Shanghai ... LIST OF ABBREVIATIONS ACA anticardiolipin antibodies ACR American College of Rheumatology ACHA anticholesterol antibody aCL anticardiolipin antibodies ANA antinuclear autoantibody ANP atrial natriuretic...

Identification and characterization of iron homeostasis related genes and HCC down regulated mitochondrial carrier protein (HDMCP), a novel liver specific uncoupling protein in human hepatocellular carcinoma (HCC

... IDENTIFICATION AND CHARACTERIZATION OF IRON HOMEOSTASIS- RELATED GENES AND HCCDOWN -REGULATED MITOCHONDRIAL CARRIER PROTEIN (HDMCP), A NOVEL LIVER- SPECIFIC UNCOUPLING PROTEIN IN HUMAN HEPATOCELLULAR ... 5’-RACE β2m mitochondrial membrane potential 5'-rapid amplification of cDNA ends β2-microglobulin aa AAH AFP ALAS ANOVA ATP amino acid atypical adenomatous hyperplasia α-fetoprotein δ-aminolevulinate ... value of protein induced by vitamin K absence (PIVKAII) and hepatoma -specific band of serum gamma-glutamyl transferase (GGTII) as hepatocellular carcinoma markers complementary to alphafetoprotein...

Identification and characterization of protein kinase CK2 as a novel interacting protein of neuronal CDK5 kinase and its functional role in microtubule dynamics

... acidic proteins (Hathaway and Traugh, 1982) Two distinct casein kinases have been found in many different cell types They have been designated casein kinase (CK1) and casein kinase (CK2) according ... Ca2+/calmodulin-dependent protein kinase II cAMP cyclic adenosine monophosphate Cdk cyclin-dependent kinase Cdk5 cyclin-dependent kinase cDNA complementary deoxyribonucleic acid CK1 casein kinase CK2 casein kinase ... interacting proteins Using the former approach, the catalytic α subunit of protein kinase CK2 (formerly known as casein kinase 2) was isolated from rat brain extracts The direct associations of CK2 with...

Xem thêm