Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 6
Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 1
... et al., 19 99) 10 0! Table 13 : Changes to ID2 protocol for expression and purification of ID2 and ID3 mutants 10 3! Table 14 : ID1 & ID2 constructs and their theoretical biochemical ... al., 19 90, Biggs, et al., 19 92, Christy, et al., 19 91, Riechmann, et al., 19 94, Sun, et al., 19 91) The mammalian family consisted of four members, namely ID1, ID2, ID3 and ID4 (Norton, 2000) and ... al., 19 99) 10 4! Table 15 : Changes to ID2 protocol for expression and purification of ID1 and ID3 HLH domains 10 4! ! vi! LIST OF FIGURES Figure 1: Hydrophobic core packing of...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 2
... cDNA (bp) AA start AA end #AA pI MW (kDa) 4 02 177 24 6 339 21 9 28 8 Construct 24 1 24 134 82 82 113 82 82 134 59 82 113 73 96 7.8 6.1 8.8 9 .2 6.1 8.8 14.9 6.8 9.3 12. 7 8.3 10.8 Full Length HLH24- 82 ... structure of ID2 3) Analyze the structure of ID2 and look at similarities and differences to other HLH-containing proteins including ID3 ! 20 ! 4) Determine differences in binding between ID1, ID2 and ... (lane S) at 17°C, HLH24- 82- L insoluble (lane P) at 30°C, HLH24- 82- L soluble (lane S) at 30°C, HLH24- 82- L insoluble (lane P) at 17°C, HLH24- 82- L soluble (lane S) at 17°C, N-HLH113 insoluble (lane...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 3
... marker (lane M, kDa) N-HLH82-L (gel A, lane 1), HLH24-82-L (gel B, lane 2), HLH24-82-L-Se-Met (gel C, lane 3) ! 38 ! 3. 3 Protein Identification The purified samples were excised from the gel and analyzed ... SILSLQASEF PSELMSNDSK ALCG Match to: Q53H99_HUMAN Score: 17248 Inhibitor of DNA binding variant (Fragment).- Homo sapiens (Human) Found in search of C:\Documents and Settings\Administrator\Desktop\Marie\1D2_BHLH.RAW ... expression and purification (A) Elution profile of nickel-bead affinity and desalting chromatography (B) SDS-PAGE: marker (lane 1), sample before induction (lane 2), pooled desalted fractions (lane 3) ,...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 6
... solution and was abandoned in favour of the higher resolution dataset of the longer form of ID2, N-HLH82-L The strategy for running MR was to use 1-2 ensembles with the Se-Met dimer and monomer ... was composed of a 4-helix bundle refined to 2.1Å with an Rfree value of 25% and no Ramachandran outlier (Table 10, Figure 13) PyMol (DeLano, 2002) was used for generating all the structural figures ... omit maps Random assignment of 10% of the reflections to the Rfree set was used for cross-validation Further model building and refinement was done manually by iterative X,Y,Z coordinate and isotropic...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 7
... mutagenesis was done on Y71 and Q76 Single mutations to change the size and charge of the residues were Y71A, Y71F, Q76A and Q76D (Table 5) Since these interactions were not found in any of the non-ID homodimers ... consisting of residues M39, L46, L49, M62, I69, I72, L75 (Figure 16A), all of which had equivalent buried residues in the structures of E 47 (Ellenberger, et al., 1994), MYOD1 (Ma, et al., 1994) and E 47- NeuroD1 ... contact An equivalent contact for Q76.cA-Y71.cB was found in the E 47- NeuroD1 structure at E 47. E596NeuroD1.Y149 (Longo, et al., 2008) Although Q76 in ID2 formed a shared hydrogen bond between L49 and...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 8
... red arrow) and ID3-Q66K did not produce any soluble protein, thus suggesting the importance of Q66 specifically to ID3’s stability and solubility Figure 17: Loop region mutants of ID2 and ID3 SDS-PAGE: ... ID2 and ID3 and its mutation in ID1 led to a complete loss of binding to MYOD1 and E47 (Pesce, et al., 1993) Since prolines were known to disrupt secondary structures, such as alpha helices, and ... could explain the rigidity of the loop in previous studies where interchanging residues ID1.L76 (ID2.I53) and ID1.Q 78 (ID2.Q55) led to total loss and partial loss of binding, respectively, to...
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Structural characterization and biochemical analysis of ID2, an inhibitor of DNA binding 9
... Edlund, T and Walker, M D ( 199 1) "Distribution and characterization of helix-loop-helix enhancer -binding proteins from pancreatic beta cells and lymphocytes", Nucleic Acids Res, 19, 3 893 -9 Atchley, ... Hernandez, M C., Kuo, W L and Israel, M A ( 199 8) "The mouse Id2 and Id4 genes: structural organization and chromosomal localization", Gene, 222, 2 29- 35 80 Martinsen, B J and Bronner-Fraser, M ( 199 8) ... A and Kealey, T (2000) "Id proteins are dynamically expressed in normal epidermis and dysregulated in squamous cell carcinoma", Cancer Res, 60, 592 9-33 63 Langlands, K., Yin, X., Anand, G and...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The ... conclusion, the IL-10 gene from carp has been isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of ... Fig Hydropathy plot of putative IL-10 proteins from carp, torafugu and human The x-axis denotes the residue position and the y-axis represents hydrophobicity The hydrophobicity analysis was carried...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
... comprehensive proteomics analysis of 14-3-3-binding proteins under physiological conditions as compared with apoptosis stimulation, with the aim of increasing our knowledge of the role of 14-3-3 proteins ... changes in 14-3-3-binding patterns in HeLa cells during C2-ceramideinduced apoptosis With the aim of further analyzing the role of 14-3-3 proteins in apoptosis, an evaluation of the ability of proteins ... role of 14-3-3 proteins in C2-ceramide-induced apoptosis, probably through regulation of already known apoptosis- related 14-3-3-binding proteins, some of them most likely still to be identified 14-3-3-binding...
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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx
... 5¢-CCTGATCGACGCACGAGT-3¢ and GT1-R, 5¢-TTTTGCAAGTCATAGTAATCAGTTT-3¢ for GTcDNA1 (2150 bp); and GT2-F, 5¢-CACCAGCAACTAC CTGATCGA-3¢ and GT2-R, 5¢-CACAAAATATGCTT CCAAGTGC-3¢ for GT-cDNA2 (3043 bp) RNA isolation and ... revealed two putative polyadenylation (ATTAAA) signals: one is located 18 bp upstream from the poly (A) of GT-cDNA1 and another is located 11 bp upstream from the poly (A) of GT-cDNA2 (data not ... 5¢-RACE and the two alternate 3¢-ends of exon 12 were deduced by 3¢-RACE, and are delineated by the full-length cDNA clones, GT-cDNA1 and GT-cDNA The two putative polyadenylation sites (ATTAAA) are...
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Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
... pattern of expression of a gene [34–39] To evaluate the relevance of the leader intron in cardosin expression, we deleted it from the 5¢-flanking region of the genes (Fig 8) The deletion of the cardosin ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... that the F-11 cells not only express the rat VRL-1, but also the mouse VRL-1 and that the mouse variant is derived from the F-11 parental cell line N18TG2 This finding is of interest when VRL-1 ... A, B and D of F-11 cells again separated into two bands The slower migrating band always corresponded to the rat brain control, and the faster migrating band to the N18TG2 control (Fig 5) The ... characteristics of the capsaicin sensitive Vanilloid receptor VR1 transiently transfected in the rat dorsal root ganglia derived cell line F-11, a hybridoma of mouse neuroblastoma and rat dorsal root ganglion...
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Báo cáo y học: " Complete coding sequence characterization and comparative analysis of the putati" docx
... drafted the manuscript SP and KS participated in the sequence alignment PL and YP participated in the design of the study and performed the data statistical analysis YP conceived of the study in ... only 64% sequence identity with the other HRV-Cs HRV-CU072 coding sequence analysis To investigate the molecular characteristics of the putative new HRV-C strain, we performed comparative analysis ... compatibility matrix (PCM) analysis is a computational method used to investigate the phylogenetic relationship of the sequences to be analyzed The PCM plot of nucleotide sequence alignment in intraand...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI