... transgenic rice genome. The results of PCRs would confirm the appearance of the AtAOS2 gene in the transgenic rice genome. 2. MATERIALS AND METHODS 2.1. Extraction of rice DNA The leaves of one-month-old ... Kim) In this study, a set of PCRs was carried out to detect the hygromycin resistance gene, CaMV35S promoter (the promoter located in front of the AtAOS2 gene), and the AtAOS2 gene in the transgenic ... (CP), microbody (MB), mitochondria (MC). In order to increase the level of endogenous JA in rice, we try to over express AOS genes by transforming AtAOS2 gene to the rice genome ...