... Ca21-binding to p26olf is EF-B, EF-A, EF-C and EF-D. In these EF-hands, EF-B and -D are the p26olf-specificEF-hands and probably show high affinity for Ca21-binding,while EF-A and EF-C are S100-specific ... helix contentwas attained by binding of two Ca21. Interestingly, in themutants which have only three Ca21-binding sites, thebinding of two Ca21was sufficient to induce more than 70%of ... Ca21-binding to each EF-hand (named EF-A,-B, -C and -D from the N-terminus of p26olf), we examinedCa21-binding in wild-type p26olf and also in its mutants in which a glutamate at the –z coordinate...